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Nucleotide Sequence Assessment of Four ORFs of Citrus Tristeza Virus: Evidence of Recombination

Phyton-International Journal of Experimental Botany 2023年第3期92卷 691-705页

作者： Adel A.Rezk Hala A.Amin Department of Agricultural Biotechnology College of Agricultural and Food ScienceKing Faisal UniversityAl-Ahsa31982Saudi Arabia Virus and Phytoplasma Research Department Plant Pathology Research InstituteAgricultural Research Center(ARC)Giza12619Egypt

Citrus Tristeza Virus(CTV),usually occurs in nature as a mixture of *** naturally infected citrus(Citrus sinensis)trees grafted on sour orange rootstock were collected from three citrus growing governorates in Egypt(Sharqia,Qalyubia and Garbia).In this study,RT-PCR,Single-Strand Conformation Polymorphism(SSCP)and nucleotide sequence analysis were used for four independent CTV genomic regions(p65,p18,p20,and p23)to detect and assess the sequence and genetic variabilities among CTV Egyptian *** products(650 bp)for the CTV p23 gene obtained from the selected isolates were used for the SSCP analysis and DNA *** patterns of p23 gene for individual isolates yielded different complex haplotype *** sequence analysis of p23 region amplified from six isolates under study revealed that p23 shared high nucleotide identity 98.7%with T36 isolate from USA,*** analysis of p23 gene indicated a close evolutionary relationship between all examined isolates and Qaha isolate(T36 isolate group),suggesting that they may have originated from closely related *** sequence analysis of the three genes located on CTV 3′-coterminal overhang,p18,p20 and p65,amplified from isolate A3,Sharqia governorate,revealed that the p18,p65,and p20 genes were related to the T3-KB isolate from South Africa with 99%–100%sequence *** relationship analysis for p65,p18 and p20 ORFs clustered the current A3 isolate with T3 genotype *** recombination analysis identified three of six isolates from Sharqia,and Garbia as potential recombinant for p23 *** isolates T36 and T3 were identified as major donors for recombination events in isolate *** results concluded that p23 ORF likely to be as a hotspot region for recombination and originated through recombination *** current study indicated that recombination is an important factor for the origin of CTV strains in Egypt.

关键词： Citrus Tristeza Virus hotspot region phylogenetic relationship analysis sequence comparison SSCP analysis

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Detecting Community Structure of Urban hotspot regions

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第十一届中国卫星导航年会

作者： Rui Chen Mingjian Chen Wanli Li Naikun Guo Information Engineering University

The travel behavior of residents is influenced by environment factor such as urban transportation system and administrative division. In turn, users equipped with navigation devices act as sensors det

关键词： navigation big data hotspot region clustering community detection

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