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A culture-dependent survey of thermophilic bacteria from hot springs in Xiamen area in China

Acta Oceanologica Sinica 2009年第1期28卷 62-71页

作者： YANG Bo OUYANG Jianping AO Jingqun CHEN Xinhua Key Laboratory of Marine Biogenetic Resources Third Institute of Oceanography State Oeeanie Administration Xiamen 361005 China

Microbes are believed to play important roles in ecosystem function in many *** hot springs of Xiamen Island are close to the Xiamen Sea,and may have some characteristics different from those of inland hot *** living in the hot springs of Xiamen may have new ***,little is known about microbial communities of hot springs close to the Xiamen Sea.A culture-dependent survey of microbial population in the Xiamen hot springs was performed by using an approach combining total cellular protein profile identification and 16S rRNA gene sequencing.A total of 328 isolates of bacteria were obtained from liquid and sediment samples from the Xiamen hot springs,including neutrophilic thermophilic bacteria and moderately thermophilic *** thermophilic bacteria,which grow at a temperature range of 55-90℃ including Rhodothermus marinus（Strain 1）,Thermus thermophilus（Strain 2）,Thermus thiopara（Strain 3）,Geobacillus stearothermophilus（Strain 4）,Geobacillus thermoleovorans（Strain 5）,and Pseudomonas pseudoalcaligenes（Strain 6）,were recovered by 2216E *** thermophilic acidophiles,which can grow at temperatures above 50℃ and a pH range of 1.8-3.5 such as Alicyclobacillus acidoterrestris（Strain 8）,Sulfobacillus acidophilus（Strain 9）,and Sulfobacillus thermosulfidooxidans（Strain 10）,were isolated on selective solid medium containing sulfur and Fe2＋.Among these strains,Rhodothermus marinus,Thermus thermophilus and Geobacillus stearothermophilus are not only thermophiles,but also *** bacterium strain（Strain 6） shared 99% nucleotide sequence homology with Pseudomonas pseudoalcaligenes on the 16S rRNA gene sequence,but was quite different from Pseudomonas pseudoalcaligenes in biological characteristics,suggesting that it may represent a novel thermophilic *** indicated that various species of neutrophilic thermophiles and moderately thermophilic acidophiles were widely distributed in the Xiamen

关键词： Xiamen sea hot springs thermophilic bacleria moderately thermophilic acidophiles cuhivable microbial community

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Distribution of glycerol dialkyl glycerol tetraethers in Tibetan hot springs

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Geoscience Frontiers 2012年第3期3卷 289-300页

作者： Liu He Chuanlun L.Zhang Hailiang Dong Bin Fang Genhou Wang State Key Laboratory of Marine Geology Tongji UniversityShanghai 200092China Department of Marine Sciences the University of GeorgiaAthensGA 30602USA Department of Geology Miami UniversityOxfordOHUSA Geomicrobiology Laboratory the Geological Lab CenterChina University of GeosciencesBeijing 100083China School of Water Resources and Environment China University of GeosciencesBeijing 100083China

Isoprenoidal glycerol dialkyl glycerol tetraethers （iGDGTs） from the Gulu hot springs （23--83.6 ℃, pH 〉 7） and Yangbajing hot springs （80-128 ℃, pH 〉 7） were analyzed in order to investigate the distribution of archaeal lipids among different hot springs in Tibet. A soil sample from Gulu was incubated at different temperatures and analyzed for changes in iGDGTs to help evaluate whether surrounding soil may contribute to the iGDGTs in hot springs. The sources of bacterial GDGTs （bGDGTs） in these hot springs were also investigated. The results revealed different profiles ofiGDGTs between Gulu and Yangbajing hot springs. Core iGDGTs and polar iGDGTs also presented different patterns in each hot spring. The PCA analysis showed that the structure of polar iGDGTs can be explained by three factors and suggested multiple sources of these compounds. Bivariate correlation analysis showed significant positive correlations between polar and core bGDGTs, suggesting the in situ production of bGDGTs in the hot springs. Furthermore, in the soil incubation experiment, temperature had the most significant influence on concentration of bGDGTs rather than iGDGTs, and polar bGDGTs had greater variability than core bGDGTs with changing temperature. Our results indicated that soil input had little influence on the composition of GDGTs in Tibetan hot springs.

关键词： Glycerol dialkyl glyceroltetraethers (GDGTs) Tibet hot springs Branched and isoprenoidtetraethers (BITs) TEX86

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Geochemical behavior of lithium isotopes in a small mountainous river of the Tibetan Plateau:A case study from Niyang River

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Science China Earth Sciences 2023年第8期66卷 1853-1864页

作者： Junwen ZHANG Yani YAN Zhiqi ZHAO Congqiang LIU School of Earth Science and Resources Chang’an UniversityXi’an 710054China Institute of Surface-Earth System Science Tianjin UniversityTianjin 300072China

Lithium(L_(i))and its isotopes are potential tracers of silicate weathering in river ***,the relationship between the Li isotopic composition(δ~7Li)in rivers and silicate weathering intensity remains *** study analyzed the Li concentration and isotopic composition in river waters from the Niyang River,southern Tibetan *** results show that these samples have significantly variable Li concentrations(0.31–7.4μg/L)andδ~7Li values(+7.0–+20.7‰,n=28),and highδ~7Li values are found in several *** indicate that dissolved Li in river water is predominantly derived from silicate weathering and geothermal *** the exception of certain tributaries,geothermal water contributes 68%to 85%of the dissolved *** waters have lowδ~7Li values(-0.9–+2.9‰)in the Tibetan *** in the proportional contribution of dissolved Li in river samples from silicate weathering and geothermal water may be the main reason for the spatiotemporal variation in riverineδ~7Li *** samples have higherδ~7Li values when the dissolved Li in the water samples is mainly derived from silicate weathering contributions,and lower values when the contribution from geothermal waters is ***,the interaction of dissolved Li from geothermal water with secondary minerals results in Li isotopic fractionation,which may contribute to variations in river waterδ~*** is accepted that the lower weathering intensity in orogenic(or mountainous)belts compared to floodplains is the main controlling factor for lowerδ~7Li values in the *** study indicates that geothermal water input may cover the Li isotope signal of silicate weathering in river water,which in turn,affects the accurate understanding of the relationship between riverineδ~7Li values and the silicate weathering ***,whether the lowerδ~7Li values of river waters in hydrothermal-rich orogenic belts are mainly controlled by the regional weathering intensi

关键词： Lithium isotopes Silicate weathering hot springs Tibetan Plateau

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A state-of-the-art review on geothermal energy exploration in Morocco: Current status and prospects

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Deep Underground Science and Engineering 2024年第3期3卷 302-316页

作者： Redouane Meryem Khalis Hind Haissen Faouziya Sadki Othman Berkat N.Eddine Raji Mohammed Faculty of Sciences Ben M'sick CasablancaMorocco Faculty of Sciences Dhar El Mahraz FezMorocco National Office of Hydrocarbons and Mines RabatMorocco

In the last few decades, addressing the global challenge of implementation of strategies for renewable energy and energy efficiency has become ***, since 2009, has made a steadfast commitment to sustainability, with a particular focus on advancing the development of renewable energy resources. A comprehensive strategy has been formulated, centering on utilizing the country's energy potential to drive progress in this vital sector. Morocco is considered a country with abundant thermal water, indicating deep reservoirs with significant hydrothermal potential. Geothermal zones were selected based on the abundance of hot springs where water temperatures were high and geothermal gradients were significant. The abundance and importance of hot springs, combined with recent volcanism and ongoing non-tectonic activity linked to alpine orogeny, strongly suggest that these regions are promising reservoirs for geothermal energy. This great potential also extends to neighboring countries. In northeast and south Morocco, the temperature of thermal water ranges from 26 to 54℃. This study serves as an inclusive review of the geothermal potentialities in Morocco.

关键词： geothermal gradients hot springs hydrothermal potential Morocco renewable energy sustainability

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Health effects of natural spring waters:A protocol for systematic reviews with a regional case example

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Journal of Integrative Medicine 2015年第6期13卷 416-420页

作者： Jessica Stanhope Philip Weinstein Angus Cook School of Population Health The University of Western Australia School of Biological Sciences The University of Adelaide School of Pharmacy and Medical Sciences The University of South Australia

BACKGROUND： Spring water therapies have been used since at least 1550 BC. Despite the growing body of evidence supporting these therapies for a range of conditions, including musculoskeletal, dermatological, respiratory and cardiovascular conditions, they do not currently form part of mainstream healthcare in many countries. The protocol established in this paper aims to support systematic reviews that examine the health outcomes associated with human exposure to regional spring waters, using the Australia and New Zealand context as a case study. METHODS/DESIGN： The protocol searches for studies in eight health/medical databases, searches three local health/medical journals, and includes forwards and backwards searching. Standard systematic review methods are used including： specifying pre-deterrnined inclusion criteria and data management plans, appraising the studies for bias, and allocation to a hierarchy of evidence. DISCUSSION： The protocol supports a review and comprehensive synthesis of the current evidence regarding the health effects of natural spring water, and can be adapted for reviews in other regions. From this evidence, recommendations regarding practice and future research can be made on the therapeutic role of spring water.

关键词： hot springs Australia New Zealand review study protocol

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