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Propofol blocks apoptosis and Bcl-2 and Bax expression induced by hypoxia-reoxygenation in primary cultures of rat hippocampal astrocytes

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Neural Regeneration Research 2009年第7期4卷 518-522页

作者： Qing Li Juying Liu Long Zhou Chengming Qin Department of Anesthesiology Taihe Hospital of Yunyang Medical Coflege Shiyan 442000 Hubei Province China

BACKGROUND： Cerebral hippocampal astrocytes are more sensitive.to ischemic injury than neurons. Hypoxic-ischemic brain injury induces profound astrocyte apoptosis, and propofol may protect against astrocyte apoptosis. OBJECTIVE： To verify the protective effects of propofol against astrocyte apoptosis and to investigate anti-apoptotic Bcl-2 and pro-apoptotic Bax expression in primary cultures of rat hippocampal astrocytes exposed to hypoxia-reoxygenation for different periods of time following propofol treatment. DESIGN, TIME, AND SETTING： In vitro neural immunocytochemistry was performed at the Central Laboratory of Yunyang Medical College between September 2007 and March 2008.MATERIALS： A total of 30 Wistar rats, aged 1-3 days, wJth equal numbers of males and females, were included for isolation and culture of .hippocampal astrocytes. METHODS： hippocampal astrocytes were purified and cultured for 3 weeks and treated with four culture conditions： 50 μL Hank＇s solution （normal control）; 0.2 mL/L Intralipid; 50 μL Hank＇s solution for 10 minutes followed by hypoxic incubation for 4 hours and normoxic incubation for 12, 24, 36, 48, 60 or 72 hours; propofol （250 μmol/L final） for 10 minutes followed by hypoxic incubation for 4 hours and normoxic incubation for 12, 24, 36, 48, 60 and 72 hours. MAIN OUTCOME MEASURES：（1） Morphologic changes in hippocampal astrocytes. （2） Levels of astrocyte apoptosis and Bcl-2 and Bax expression. RESULTS： Hypoxia and reoxygenation increased apoptosis over time, with Bcl-2 expression peaking at 24 hours and decreasing gradually （P 〈 0.01 ）; Bax expression peaked at 72 hours （P 〈 0.01）; the ratio of Bcl-2/Bax was 1.4, 0.8, and 0.6, respectively, at 24, 48 and 72 hours. Non-apoptotic astrocytes showed significant proliferation and swelling. Propofol treatment decreased apoptosis after hypoxia-reoxygenation （P 〈 0.01）, as well as Bct-2 and Bax expression （P 〈 0.05, P 〈 0.01）, with Bcl-2/Bax ratios of 1.6-1.8. Propofol treatmentalso blocked astrocyte proliferation and swelling. N

关键词： propofol hippocampal astrocyte apoptosis hypoxia and re-oxygenation Bcl-2 Bax

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The effect of Modified Wen-Dan decoction on hippocampal astrocytes in depression model rats

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TMR Pharmacology Research 2021年第3期1卷 41-47页

作者： Li-Ping Zhang Shen-jun Wang Ting Xue Lei Xu Yun-Sha Zhang Ying Chen Jun Yang Rui-Wen Song Xue-Yan Wang Tianjin University of Chinese Medicine TianjinChina301617

Background:This study aimed to observe the effect of Modified Wen-Dan decoction on hippocampal astrocytes in depression model rats.Methods:A total of 48 rats were randomly divided into a control group,a model group,a Modified Wen-Dan decoction group(referred toas the traditional Chinese medicine group),and a fluoxetine control group(referred to as the Western medicine group).The control group was provided with a free diet and without any stimulation,while the remaining three groups were solitarily housed incombination with chronic unpredictable mild stress stimuli to replicate the depression rat model.The rats were subjected to the open-field and sucrose preference experiments for behavioral observation,and q-PCR was used to detect the mRNA of specific markers of hippocampal astrocytes,glial fibrillary acidic protein,and S100 calcium-binding proteinβ(S100β)in each group.Immunohistochemistry was used to observe the protein expression of glial fibrillary acidic protein and S100βin thehippocampus of rats of each group.Results:The behavioral results showed that,compared with the control group,the rats in the model group showed a decrease in the score of the open-field experiment and in the rate of sucrose preference(P0.05).The q-PCR results showed that the mRNA and protein expression levels of GFAP and S100βwere downregulated in the model group compared to that of the control group(P<0.05).Compared with the model group,the mRNA expression levels of glial fibrillary acidic proteinand S100βwere upregulated in the traditional Chinese medicine group as well as in the Western medicine group(P<0.05).No significant difference between the Chinese and Western medicine groups was found.Conclusion:The mechanism of the antidepressant effect of Modified Wen-Dan decoc

关键词： Modified Wen-Dan decoction hippocampal astrocyte Depression Chinese medicine

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Protective effects of dihydromyricetin on primary hippocampal astrocytes co-cultured with high glucose plus substance P and corticosterone

Protective effects of dihydromyricetin on primary hippocampa...

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2019中国生理学会学术年会暨张锡钧基金第十五届全国青...

作者：葛会香 Mengyun Sun Xingyu Wei Hongcheng Tu Ruxin Chen Miao Ye Cancan Yin Ruoyu Huang Wenhao Shen Wei Xiong Yun Gao Department of Physiology Basic Medical CollegeNanchang University Medical College of grade 2017 Nanchang University Affiliated Stomatological Hospital of Nanchang University Jiangxi Provincial Key Laboratory of autonomic nervous function and disease

Activated astrocytes are key players in diabetic neuropathic pain and depression.Dihydromyricetin（DHM） is a natural dihydroflavonoid.Our aim was to investigate whether DHM has a protective effect on the primary hippocampal astrocytes co-cultured with high glucose（HG）plus substance P（SP） and corticosterone（CORT）.Co-culture of HG+SP+CORT resulted in damage to the primary cultured hippocampal astrocytes, but DHM treatment could alleviate the impairment.Using qPCR, Western blot, immunofluorescence, We found that HG+SP+CORT increased the expression of P2 X7 receptor in primary cultured hippocampal astrocytes, whereas DHM treatment significantly reversed the change.Culture of the primary hippocampal astrocytes with HG+SP+CORT was able to elevate the level of TNF-α, IL-1β, free Ca 2+, and ERK1/2 phosphorylation.These increases could be inhibited by DHM or P2 X7 shRNA treatment.Furthermore, DHM significantly reduced the P2 X7 agonist activated currents in HEK293 cells transfected with the P2 X7 receptor.These findings suggest that DHM can protect the primary hippocampal astrocytes co-cultured with HG+SP+CORT form P2 X7 receptor-mediated damage.Co-culture of cells and HG+SP+CORT might be a good cell model for exploring pain and depression injury in diabetes mellitus.

关键词： Dihydromyricetin diabetic neuropathic pain depression primary culture hippocampal astrocyte P2X7 receptor

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