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Cloning and characterization of cotton heteromeric acetyl-CoA carboxylase genes

Progress in Natural Science:Materials International 2007年第12期17卷 1412-1418页

作者： Qiao Zhixin and Liu Jinyuan(Laboratory of Molecular Biology and Protein Science Laboratory of the Ministry of Education, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China) Laboratory of Molecular Biology and Protein Science Laboratory of the Ministry of Education Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China

heteromeric acetyl-coenzyme A (CoA) carboxylase (accase) catalyzes the formation of malonyl-CoA from acetyl-CoA. It plays an essential role in fatty acid synthesis in prokaryotes and most of plants. The heteromeric accase is composed of four subunits: biotin carboxylase (BC), biotin carboxyl carrier protein (BCCP), and α- and β-subunits of carboxyltransferase (α- and β-CT). In this study, we cloned five novel genes encoding the subunits of heteromeric accase (one BC, BCCP and β-CT, and two α-CTs) from cotton (Gossypium hirsutum cv. zhongmian 35) by RACE-PCR. The deduced amino acid sequence of these cDNAs shares high similarity with other reported heteromeric accase subunits. The phylogenetic analysis indicated that the different subunits of heteromeric accase were grouped in a similar pattern. Southern blot analysis revealed the milti-copy patterns of these heteromeric accase genes in cotton genome. Semi-quantitative RT-PCR demonstrated that heteromeric accase genes were constitutively expressed in all of the cotton tissues, but the transcripts accumulated at a relatively low level in roots. To our knowledge, this is the first report about characterization of the heteromeric accase genes in cotton.

关键词： heteromeric accase cotton gene cloning fatty acid synthesis.

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