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Ethanol extract of Ardisiae Japonicae Herba inhibits hepatoma carcinoma cell proliferation in vitro through regulating lipid metabolism

引用

Chinese Herbal Medicines 2021年第3期13卷 410-415页

作者： Xue Gong Huan-tian Cui Yu-hong Bian Yu-ting Li Yang-xue Wang Yan-fei Peng Wei-bo Wen Kuan Li Hong-wu Wang Zhai-yi Zhang Fang Zheng Tianjin University of Traditional Chinese Medicine Tianjin 301617China Shandong Provincial Key Laboratory of Animal Cell and Developmental Biology School of Life SciencesShandong UniversityQingdao 250100China First Affiliated Hospital of Yunnan University of Traditional Chinese Medicine Kunming 650021China

Objective:The aim of this study is to discover the possible working mechanisms of Ardisiae Japonicae Herba(AJH)on hepatoma carcinoma(HCC).Methods:In this study,ethanol extract of AJH was prepared and used to treat HCC cell in ***,a genomic wide RNA sequencing(RNA-seq)was performed to screen deregulated genes in HCC cells after the treatment of AJH *** gene and protein expression related to lipid metabolism in HCC cells were also investigated to validate the results obtained from ***:AJH extract could inhibit HCC cell proliferation in ***-seq analysis has identified 1,601 differentially expressed genes(DEGs,fold change≥2.0 or fold change≤0.5,P<0.05)in HCC after AJH extract treatment,which included 225 up-regulated genes and 1,376 down-regulated *** pathway analysis of DEGs demonstrated that lipid metabolism was a potential pathway related to AJH *** agreement with the RNA-seq data,q PCR and Western-blot analysis indicated that expression of genes and proteins related to lipid metabolism(SREBP1,ACC,ACLY and FASN)were significantly downregulated in AJH treatment group as compared with the control ***,AJH extract could also decrease lipid contents and cellular free fatty acid levels in HCC ***:Ethanol extract of AJH could inhibit HCC cell proliferation in vitro,the possible mechanism may be related to the inhibition of lipid metabolism.

关键词： Ardisiae Japonicae Herba hepatoma carcinoma lipid metabolism RNA-seq

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