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HbV genotype characterization and distribution in patients with HbV-related liver diseases in Zhejiang Province, P. R. China: possible association of co-infection with disease prevalence and severity

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Hepatobiliary & Pancreatic Diseases International 2005年第4期4卷 535-543页

作者： Edward Zumbika Institute of Infectious Diseases the First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003People's Republic of China

bACKGROUND: There are 8 well-documented genotypes of hepatitis b virus (HbV) at this time point. Genotyping can be accomplished based on a partial sequence of hepatitis b virus (HbV) genome such as the pre-S or S gene. Several methods have been developed and used for HbV genotyping including direct sequencing, restriction fragment length polymorphism, line probe assay and enzyme-linked immunoassay. Recently, a novel, rapid and cost-effective genotyping method based on PCR amplification assay using type-specific primers that can identify all six major genotypes has been developed. This study was undertaken to characterise HbV genotypes and investigate the association between the prevalence of different genotypes and the severity of HbV-induced liver diseases. METHODS: Serum samples from carriers of HbV and patients with HbV-related liver diseases from Zhejiang Province were screened for viral serological markers using commercially available radioimmunoassay (RIA) and enzyme linked immunosorbent assay (ELISA) kits. Serum HbV DNA load was determined by real-time detection PCR. A type-specific primer based the nested-PCR method was employed in the HbV genotyping. The genotype results obtained were confirmed by direct sequencing of nested PCR amplicons of the pre-S region. Ten samples of each genotype (b and C) were sequenced. RESULTS: The survey on a cohort of 125 HbV carriers in and around Hangzhou City, Zhejiang Province showed the existence of HbV genotypes A (0.8%), b (48%), C (40.8%), D (0.8%), mixed b and C (9.6%) and an absence of E and F genotypes. Distribution of HbV genotypes in patients with liver diseases revealed a statistically insignificant higher prevalence of genotype b in mild chronic hepatitis (CH). Among the three genotypes b, C and mixed b/C infections 11 (73.3%), 3 (20%) and 1 (6.7%), (Pbjects with moderate CH, genotype b was significantly predominant. The infection patterns for genotypes b, C and b/C mixed in (i) liver c

关键词： hepatitis b virus chronic hepatitis b liver cirrhosis hepatocellular carcinoma hepatitis b e antigen hepatitis b virus DNA load viremia hepatitis b genotypes liver function tests alanine transaminase aspartate transaminase real-time detection PCR

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