检索结果-南通市图书馆

AB030. Characterization of the interactions between hepatic stellate cells and tumor cells during uveal melanoma metastatic progression

引用

Annals of Eye Science 2018年第1期 436-436页

作者： Léo Piquet William Pelletier Peter Gerges Julie Bérubé Solange Landreville Centre de recherche du CHU de Québec-UniversitéLaval Hôpital du Saint-SacrementQuébecQCCanada Centre de recherche en organogénèse expérimentale de l’UniversitéLaval/LOEX QuébecQCCanada Département d’ophtalmologie et d’ORL-CCF Facultéde médecineUniversitéLavalQuébecQCCanada

Background:Uveal melanoma(UM)is the most common primary eye tumor in adults,and the most frequent site of malignant transformation of the melanocytes after the *** spreads to the liver in half of the cases,and the death rate following the report of metastasis is 92%at 2 *** tropism of UM cannot simply be explained by the blood circulatory system organization,and illustrates the“seed and soil”hypothesis that describes an interaction between tumor cells(seed)and a specific microenvironment(soil).We decided to focus our study on the synergic interaction between UM cells and hepatic stellate cells(HSC),whose role has been previously described in the metastatic progression of colon and pancreatic ***,HSC have been found surrounding UM liver metastasis,and the UM secretome contains activating cytokines of hepatic stromal *** hypothesis is that HSC provide a specific microenvironment in the liver enhancing the growth of UM cells and increasing their therapeutic *** an in vitro 3D model and an original xenograft mouse model,we aim to decipher the mechanisms of UM metastatic progression,in order to elaborate new therapeutic ***:First,using an agar coating,spheroids were generated with UM cells and were allowed to grow for 72 *** tumor spheroids were then embedded in Matrigel and the HSC conditioned medium was used to evaluate the impact of the HSC secretome on UM ***,an original in vivo xenograft mouse model was generated,in which metastatic UM cells were injected alone or with human HSC in the spleen of immunodeficient *** model allows us to evaluate in 3-6 weeks the metastatic potential of each cell population,and thus to determine the cooperation between HSC and UM cells in the ***:The HSC conditioned medium increased the invasion of UM spheroids compared to non-conditioned medium in our in vitro *** addition,UM cells inoculated in the mouse spleen alone or with human HSC we

关键词： Uveal melanoma(UM) hepatic stelllate cells-interaction metastasis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：