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An Experimental Study on the Expression of heme oxygenase-2 mRNA in Hirschsprung's Disease

Journal of Huazhong University of Science and Technology(Medical Sciences) 2002年第2期22卷 152-154页

作者： ZHU Min(朱珉) WEI Mingfa(魏明发) LIU Fang(刘芳) Department of Pediatric Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and TechnologyWuhan 430030 Department of Pediatric Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and TechnologyWuhan 430030 Department of Pediatric Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and TechnologyWuhan 430030

Summary: In order to investigate the relationship between the expression of heme oxygenase-2 (HO-2) mRNA and the pathogenesis of Hirschsprung's disease (HD), total ribonucleic acid (RNA) was extracted in the aganglionic and ganglionic segments of colon respectively from 15 cases of HD. The single-stranded cDNA of HO-2 was synthesized and further amplified by reverse transcription-polymerase chain reaction (RT-PCR). The expression of HO-2 mRNA was normal in ganglionic segments, but absent in aganglionic segments. It is concluded that the absence of HO-2 mRNA expression may be an important mechanism responsible for HD.

关键词： Hirschsprung's disease heme oxygenase-2 gene expression reverse transcription-polymerase chain reaction

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Neuroprotective mechanisms of heme oxygenase

Neuroprotective mechanisms of heme oxygenase

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中国神经科学学会第四次会员代表大会暨第七届全国学术会议

作者： David GIUS Gabriele V.RONNETT Radiation Oncology Branch Radiation Oncology Sciences ProgramNational Cancer InstituteNational Institutes of HealthBethesdaMD 20892USA Department of Neuroscience The Johns Hopkins University School of MedicineBaltimoreMD 21205USA

＜正＞heme oxygenase（HO） enzymes catalyze the breakdown of heme to iron,carbon monoxide（CO）,and biliverdin, which is rapidly converted to ***-1 is an inducible isoform that is known to provide protection against oxidative stress,whereas HO-2 and HO-3 constitutively ***-2 is the predominant isoform expressed in the brain under normal ***-2 has been implicated in protection against oxidative stress,ischemia,and traumatic brain injury. The neuroprotective effects of HO-2 have been attributed to the generation of bilirubin,which is an important radical ***,the mechanism by which HO-2 protection is *** utilized the olfactory system as a model to define the roles of HO-2 in glutathione depletion-induced oxidative injury,as olfactory receptor neurons（ORNs） express high levels of HO *** demostrated that L-buthionine-[S.R.]-sulfoximine（BSO）,an inhibitor of glutathione biosynthase,lowered glutathione levels and induced apoptosis of *** the presence of HO-1 in ORNs,HO-2 null animals displayed increased levels of neuronal death afer BSO treatment compared to wild type *** of bilirubin and cGMP were also reduced in HO-2 null *** used primary olfactory neuron culture as a model;study if neuroprotective mechanisms of HO are regulated by *** found HO inhibitor（SnPPIX） induced neuron death, bilirubin level also *** could reverse the neurotoxic actions of HO inhibitor *** further investigate the molecular mechanisms of CO-cyclic GMP pathway to protect neruons from glutathione depletion-induced oxidative *** of cGMP level decreased in ORNs of HO-2 null mice after BSO *** olfactory primary culture,HO inhibitor SnPPIX and BSO induced ORN apoptosis,levels of cGMP also decreased;A cGMP analog,8-Br-cGMP could reverse the effects of SnPPIX and BSO on neuronal *** investigation showed extracellular-regulated kinase （ERK） phosphorylation might be downstream

关键词： heme oxygenase-2 oxidative stress bilirubin cyclic GMP olfaction

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