检索结果-南通市图书馆

Chinese Journal of Integrative Medicine 1995年第2期1卷 39-42页

作者： Gao Rui-lan (高瑞兰) Xu Cong-lian (徐从连) Jin Jin-mei (金锦棉) Ma Feng-shun (马逢顺) Wang Wen-tao(王文涛) (Affzliated Hospital , Zhejiang College of Traditional Chinese Medicine , Hangzhou) (310006)Lin Zhen-chang (林振常) Liang Huai-shan ( 梁怀山) Affiliated Hospital Zhejiang College of Traditional Chinese Medicine Hangzhou The 2nd Factory of Traditional Chinese Pharmaceutics of Hangzhou Hangzhou

Ginseng is said to have beneficial effects on anemia. The proliferation effects of totalsaponins of Panax ginseng (TSPG) on hematopoietic progenitor cell in healthy individuals and 29 patientswith aplastic anemia (AA) were observed through bone marrow cultures of burst forming unit-erythroid(BFU-E) , colony forming unit-erythroid (CFU-E) and colony forming unit-granulocyte/macrophage (CFU-GM) in vitrcacompared with methyltestosterone (MT). The results suggest TSPG might prompt the prolif-eration of normal progenitor cells at a concentration of 20 g/ml. The numbers of BFU-E ,CFU-E and CFU-GM increased by 37. 8±2.9 % , 31. 4±2. 9 % and 33. 3± 4. 0 % respectively over the controls ; further-more TSPG was still useful to BFU-E,CFU-E growth without Epo in vitro, although the colony nurnberswere much lower. Otherwise MT was useless to CFUGM. Of the 29 patients with AA, 14 who respondedto MT showed sensitivity to TSPG in marrow culture (the rising rate of colony formation exceeded 30 % ) ,but immune-mediated AA (patient's peripheral blood mononucleated cell suppressed normalhematopoiesis) and stem cell decreased AA (few of colonies were formed) showed almost no expressionfor TSPG activity because of the immunological suppression system and the absence of progenitors.

关键词： total saponins of Panax ginseng hematopoietic progenitor cells aplastic anemia

Inducing effects of macrophage stimulating protein on the expansion of early hematopoietic progenitor cells in liquid culture

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2007年第13期120卷 1192-1197页

作者： MA Li-xia HUANG Yan-hong CHENG La-mei LEI Jun WANG Qi-ru Experimental Hematology Laboratory Department of Physiology Xiangya Medical School of Central South University Changsha 410078 China Department of Cardiology the First Hospital of Changsha City Changsha 410005 China

Background Macrophage stimulating protein （MSP） is produced by human bone marrow endothelial cells. In this study we sought to observe its effects on inducing the expansion of early hematopoietic progenitor cells which were cultured in a liquid culture system in the presence of the combination of stem cell factor （SCF）, interleukin 3 （IL-3）, interleukin 6 （IL-6）, granulocyte macrophage-colony stimulating factor （GM-CSF）, erythropoietin （EPO）（Cys） and MSP or of Cys and bone marrow endothelial cell conditioned medium （EC-CM）. Methods Human bone marrow CD34^＋ cells were separated and cultured in a liquid culture system for 6 days. Granulocyte-macrophage colony forming unit （CFU-GM） and colony forming unit-granulocyte, erythrocyte, macrophage, megakaryocyte （CFU-GEMM） were employed to assay the effects of different treatment on the proliferation of hematopoeitic stem/progenitor cells. The nitroblue tetrazolium （NBT） reductive test and hoechest 33258 staining were employed to reflect the differentiation and apoptosis of the cells respectively. Results MSP inhibited the proliferation of CFU-GM and CFU-GEMM in semi-solid culture and the inhibitory effect on CFU-GEMM was stronger than on CFU-GM. MSP inhibited the differentiation of early hematopoietic progenitor cells induced by hematopoietic stimulators. Bone marrow （BM） CFU-GEMM was 2.3-fold or 1.7-fold increase or significantly decreased in either Cys＋EC-CM, Cys＋MSP or Cys compared with 0 hour control in liquid culture system after 6 days. Conclusion MSP, a hematopoietic inhibitor, inhibits the differentiation of early hematopoietic progenitor cells induced by hematopoietic stimulators and makes the early hematopoietic progenitor cells expand in a liquid culture system.

关键词： macrophage stimulating protein bone marrow endothelial cell hematopoietic progenitor cells

Spaceflight and simulated microgravity suppresses macrophage development via altered RAS/ERK/NFκB and metabolic pathways

在线全文

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2021年第6期18卷 1489-1502页

作者： Lu Shi Hongling Tian Peng Wang Ling Li Zhaoqi Zhang Jiayu Zhang Yong Zhao State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijingChina University of Chinese Academy of Sciences BeijingChina Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijingChina

Spaceflight-associated immune system weakening ultimately limits the ability of humans to expand their presence beyond the earth's orbit. A mechanistic study of microgravity-regulated immune cell function is necessary to overcome this challenge. Here, we demonstrate that both spaceflight (real) and simulated microgravity significantly reduce macrophage differentiation, decrease macrophage quantity and functional polarization, and lead to metabolic reprogramming, as demonstrated by changes in gene expression profiles. Moreover, we identified RAS/ERK/NFκB as a major microgravity-regulated pathway. Exogenous ERK and NFκB activators significantly counteracted the effect of microgravity on macrophage differentiation. In addition, microgravity also affects the p53 pathway, which we verified by RT-qPCR and Western blot. Collectively, our data reveal a new mechanism for the effects of microgravity on macrophage development and provide potential molecular targets for the prevention or treatment of macrophage differentiation deficiency in spaceflight.

关键词： Microgravity hematopoietic progenitor cells Macrophage differentiation Macrophage polarization RAS/ERK/NFκB pathway

3D collagen matrices modulate the transcriptional trajectory of bone marrow hematopoietic progenitors into macrophage lineage commitment

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bioactive Materials 2022年第4期7卷 255-268页

作者： Pan Zhang Linmu Xu Jingsong Gao Guangkui Xu Yanping Song Guang Li Jingjing Ren Yunjie Zhang Cheng Yang Yu Zhang Ruiheng Xie Nu Zhang Hui Yang School of Life Sciences Northwestern Polytechnical UniversityXi’anShaanxi710072China Research Center of Special Environmental Biomechanics&Medical Engineering Northwestern Polytechnical UniversityXi’anShaanxi710072China School of Aerospace Engineering Xi’an Jiaotong UniversityXi’anShaanxi710049China Institute of Hematology Xi’an Central HospitalXi’anShaanxi710003China School of Computer Science Northwestern Polytechnical UniversityXi’anShaanxi710072China Department of Computer and Information Science University of DelawareNewarkDEUSA

Physical signals provided by the extracellular matrix(ECM)are key microenvironmental parameters for the fate decision of hematopoietic stem and progenitor cells(HSPC)in bone *** into cell-ECM interactions are critical for advancing HSC-based tissue ***,we employed collagen hydrogels and collagen-alginate hydrogels of defined stiffness to study the behaviors of hematopoietic progenitor cells(HPCs).Three-dimensional(3D)collagen hydrogels with a stiffness of 45 Pa were found to promote HPC maintenance and colony formation of monocyte/macrophage *** single-cell RNA sequencing(scRNA-seq),we also characterized the comprehensive transcriptional profiles of cells randomly selected from two-dimensional(2D)and 3D hydrogels.A distinct maturation trajectory from HPCs into macrophages within the 3D microenvironment was revealed by these results.3D-derived macrophages expressed high levels of various cytokines and chemokines,such as Saa3,Cxcl2,Socs3 and ***,enhanced communication between 3D-macrophages and other hematopoietic clusters based on ligand-repair interactions was demonstrated through bioinformatic *** research underlines the regulatory role of matrix-dimensionality in HPC differentiation and therefore probably be applied to the generation of specialized macrophages.

关键词： hematopoietic progenitor cells Collagen Matrix-dimensionality Macrophages

Changes in the number of circulating CD34＋ cells after eccentric exercise of the elbow flexors in relation to muscle damage

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Sport and Health Science 2015年第3期4卷 275-281页

作者： Ho Seong Lee Makii Muthalib Takayuki Akimoto Kazunori Nosaka Department of Kinesiologic Medical Science Dankook University Choongnam 330-714 South Korea Institute of Health and Biomedical Innovation School of Human Movement Studies Queensland University of Technology Brisbane 4000 Australia Division of Regenerative Medical Engineering Center for Disease Biology and Integrative Medicine Graduate School of Medicine University of Tokyo Tokyo 113-8654 Japan School of Exercise and Health Sciences Edith Cowan University Joondalup Western Australia 6027 Australia

Background： It has been reported that strenuous exercise increases the number of bone marrow-derived progenitor cells such as CD34＋ cells in the blood, but no previous studies have investigated the changes in circulating CD34＋ cells following resistance exercise. This study tested the hypothesis that the number of CD34＋ cells in the blood would increase after eccentric exercise of the elbow flexors, but decrease in recovery, and the magnitude of the changes would be dependent on the magnitude of muscle damage. Methods： Nine men （28.0 ± 6.6 years） performed exercises consisting of 10 sets of six maximal voluntary eccentric contractions of the elbow flexors with their non-dominant arm. Six of them performed the same exercise with the same ann 4 weeks later. Changes in indirect markers of muscle damage were measured before, within 10 min after, and at 24, 48, 72, and 96 h after eccentric exercise. Differential leukocyte counts （total leukocytes, neutrophils, lymphocytes, monocytes） and CD34＋ cells in the blood were measured before, immediately after, and at 2, 24, 48, 72, and 96 h following the exercises. Results： After eccentric exercise, significant （p 〈 0.05） decreases in maximal voluntary isometric contraction torque and increases in delayed onset muscle soreness and plasma creatine kinase activity were observed. However, no significant changes in leukocytes and CD34＋ cells were evident. The changes in muscle damage markers were significantly （p 〈 0.05） smaller following the second exercise session as compared with the first exercise session, but the changes in leukocytes and CD34＋ cells were not significantly different between sessions. Conclusion： These results did not support the hypothesis, and showed that eccentric exercise-induced muscle damage to the elbow flexors did not influence the number of circulating CD34＋ cells.

关键词： Creatine kinase hematopoietic progenitor cells Lengthening contractions Leukocytes Repeated bout effect

Chemoprotection of transfer of multidrug resistance gene into human hematopoietic progenitor cell

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2000年第6期113卷 56-59页

作者：潘凌亚童英周生吴英毛宁杨秀玉

Objective To observe the effect of the transfer of multidrug resistance gene (mdr1) into human hematopoietic progenitor cells (HPC) on the chemoprotection Methods Human CD34 + cells served as a target of mdr1 gene transfer Retroviral vector SF mdr containing human total length mdr1cDNA was introduced into packing cells GP envAM12 by liposome mediated transfection The mdr1 gene was transduced into human CD34 + cells by retroviral supernatants of packing cells The integration and expression of the mdr1 gene and its protein (P170) in transduced cells were determined by PCR, RT PCR, and flow cytometry The drug resistance of chemotherapy in transduced HPC was determined by culturing colonies Results The mdr1 gene was integrated and expressed in transduced CD34 + cells The efficiency of mdr1 gene transfer was 10%-14% Compared with untransduced controls, within a certain range of drug concentration, the number of drug resistant colony in transduced HPC for taxol, doxorubicin,VCR and VP16 were increased by 3 6±2 1 fold, 2 9±0 3 fold, 1 9±0 4 fold, and 3 5±0 5 fold, respectively Conclusion The transfer of the mdr1 gene into human HPC can increase the drug resistance of the transduced cells to corresponding chemotherapeutic drugs that may provide some degree of chemoprotection for HPC

关键词： multidrug resistance gene hematopoietic progenitor cells human chemoprotection