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第六届中国植物逆境生理学与分子生物学学术研讨会

作者： Ke-Zhen Yang,Chuan Xia,Xiao-Lei Liu,Xiao-Ying Dou,Zhao-Xia Ma,Li-Qun Chen,Xue-Qin Zhang,and De YeState Key Laboratory of Plant Physiology and Biochemistry,College of Biological Sciences,China Agricultural University,Yuanmingyuan Xilu 2,Beijing 100193,China

＜正＞In most flowering plant species,pollination and fertilization occur during the hot *** plants must have evolved a mechanism that ensures normal growth of their pollen tubes at high *** of the thermo-tolerant mec... 详细信息

关键词： Male gametophyte Pollen tube Pollination heat stress heat shock protein Arabidopsis.

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Nitric oxide involved in regulating HS induced GA biosynthesis via inhibition of aconitase in Ganoderma lucidum

Nitric oxide involved in regulating HS induced GA biosynthes...

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多彩菌物美丽中国——中国菌物学会2019年学术年会

作者： Rui Liu Ang Ren Liang Shi Jing Zhu Mingwen Zhao Key Laboratory of Agricultural Environmental Microbiology Ministry of AgricultureMicrobiology Department College of Life Sciences Nanjing Agricultural University

Ganoderma lucidum（G. lucidum） is one of the best-known medicinal basidiomycetes in the world,because it containing a wide range of immuno-modulatory and bioactive compounds. Ganoderic acid（GA） is one of the major secondary metabolites in G. lucidum, a basidiomycete with high commercial value. Previous studies have shown that heat stress（HS） increases GA biosynthesis via induced reactive oxygen species（ROS）accumulation in G. lucidum[1] and NO relieves HS-induced GA accumulation in G. lucidum[2]. To determine the mechanism that NO relieves HS-induced GA accumulation in G. lucidum, the effect of NO on the ROS content was examined under heat treatment. The results showed that NO decreased the production of MitROS under heat treatment. Further research found that NO reduced the MitROS content through inhibit the aconitase activity. In the aconitase silenced strains（Aconi）, treatment with NO donor（SNP） showed no obviously effect on the mitROS and GA content relative to the untreated sample. Combined with the prior results, these findings indicate that NO inhibits aconitase activity to reduced mitROS content and thereby relieved HS-induced GA overproduction in Ganoderma *** S-nitrosylation, the incorporation of a nitroso group to a Cys thiol, has been established as a significant route through which NO transmits its global cellular influence. To study the mechanism of the inhibition of the aconitase activity in G. lucidum, the aconitase proteins S-Nitrosylation was ***-switch technology（BST） and mass spectrometric analysis revealed that Acon was S-nitrosylated at amino acid residues, Cys 594. The substitution of Cys 594 with non-nitrosylatable Ser with site-directed mutagenesis causes the abolishes its responsiveness to the NO-reducted enzymatic activity. These findings demonstrate that NO inhibited the activity of aconitase through S-nitrosylation at Cys 594 thereby relieved HS-induced GA biosynthesis in Ganoderma lucidum..

关键词： Nitric oxide heat stress Ganoderic Acid Aconitase S-Nitrosylation

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Characterization of wheat MYB genesresponsive to high temperatures

Characterization of wheat MYB genesresponsive to high temper...

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第八届全国小麦基因组学及分子育种大会

作者： Yue Zhao Xuejun Tian Fei Wang Liyuan Zhang Mingming Xin Zhaorong Hu Yingyin Yao Zhongfu Ni Qixin Sun Huiru Peng State Key Laboratory for Agrobiotechnology and Key Laboratory of Crop Heterosis and Utilization(MOE) Beijing Key Laboratory of Crop Genetic ImprovementDepartment of Plant Genetics and BreedingChina Agricultural University

heat stress is one of the most crucial environmental factors,which reduces crop yield *** plants,the MYB family is one of the largest families of transcription factors(TFs).Although some wheat stress-related MYB TFs have been characterized,their involvement in response to high-temperature stress has not been properly *** novel heat-induced MYB genes were identified by conparison of previously established de novo transcriptome sequencing data obtained from wheat plants subjected to heat treatment;genomic and complete coding sequences of these genes were *** six TaMYBs were localized in the nuclei of wheat *** assays in yeast revealed that all six proteins act as transcriptional activators and the activation domains were attributed to the C-termini of six wheat MYB *** analysis of the six TaMYBs and the R2 R3-MYBs from Arabidopsis revealed that all six proteins belong to clades containing stress-related MYB *** genes exhibited different expression profiles in wheat tissues and in response to various abiotic stresses and exogenous abscisic acid *** Arabidopsis plants carrying TaMYB80 driven by the CaMV 35 S promoter exhibited enhanced tolerance to heat and drought stresses,which can be attributed to the increased levels of cellular *** identified six heat-induced MYB genes in *** performed comprehensive analyses for the cbned MYB genes and their gene products,including gene structures,subcellular localization,transcriptional activation,phylogenetic relationships,and expression patterns in different wheat tissues and under various abiotic *** particular,we showed that TaMYB80 confers heat and drought tolerance in transgenic *** results contribute to our understanding of the functions of heat-induced MYB genes and provide the basis for selecting the best candidates for in-depth functional studies of heat-responsive MYB genes in wheat.

关键词： abiotic stress heat stress MYB transgenic Arabidopsis wheat

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Inducible and Neuronal Nitric Oxide Synthase Inhibitors Protect Against Experimental heatstroke-induced Circulatory Shock and Cerebral Ischemic Injury

Inducible and Neuronal Nitric Oxide Synthase Inhibitors Prot...

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第八届海峡两岸心血管科学研讨会

作者：黄戊田张菁萍张明温郑伯智许淑芬林茂村 Department of Recreation and Health-Care Management Chia-Nan University of Pharmacy and Science Department of Biotechnology Southern Taiwan University Department of Electrical Engineering Southern Taiwan University Department of Surgery Chi Mei Medical Center Department of Physical Therapy Shu-Zen College of Medicine and Management Department of Medical Research Chi Mei Medical Center

＜正＞Background Circulatory shock as well as cerebral ischemia results in neuronal injury and death in animals suffering from *** levels of nitric oxide（NO） have also been associated with a number of models of circulatory shock or cerebral *** This study attempted to determine whether NO contributes to the hypotensive state and cerebral ischemic insults induced by prolonged environmental heat stress（ES）.Methods Urethane - anesthetized rats were instrumented for the measurement of mean arterial pressure（MAP）,cerebral blood flow（CBF） and neuronal damage score（NDS）,and colonic temperature （Tco）.Rats were exposed to ES（ambient temperature,43 oC） until MAP and CBF began to decrease from their peak levels,which was arbitrarily defined as the onset of *** time to death（TTD,interval between the start of ES and cardiac arrest） was also *** addition to these parameter measurements, the inducible NO synthase（iNOS）,neuronal NOS（nNOS） and endothelial NOS（eNOS） protein expression in different brain structures were assessed during *** The values of MAP,CBF and TTD after heatstroke onset were all significantly lower than those in control ***,the values of Tco and NDS were *** addition,after the onset of heatstroke,the protein expression of iNOS,nNOS and eNOS were all significantly elevated in the cortex,hypothalamus and hippocampus of rat *** pretreatment with aminoguanidine（an iNOS inhibitor） or 7 - nitro - indazole（a nNOS inhibitor）,but not L - NAME（an eNOS inhibitor）,at the start of ES,did attenuate the heatstroke - induced arterial hypotension,cerebral ischemia and neuronal damage and result in prolongation of *** Taken together,these results indicate that NO mediate the circulatory shock and cerebral ischemic injury associated with heatstroke.

关键词： cerebral ischemia neuronal damage heat stress circulatory shock

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Bacillus thuringiensis （Bt） Toxin,Gossypol,and Condensed Tannin Contents of Transgenic Bt Cotton（Gossypium hirsutum L.） Seedlings Affected by Mepiquat Chloride Application and Growth Temperature

Bacillus thuringiensis （Bt） Toxin,Gossypol,and Condensed T...

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中国棉花学会2005年年会暨青年棉花学术研讨会

作者： JIANG Lin-jian, DUAN Liu-sheng, TIAN Xiao-li, WANG Bao-min, ZHZNG Ming-cai, LI Zhao-hu(Centre of Crop Chemical Control, Department of Agronomy, State Key Lab of Plant Physiology and Biochemistry, China Agricultural University, Beijing 100094, China)

Seedling responses of two cultivars of transgenic Bacillus thuringiensis （Bt） cotton （Gossypium hirsutum L.）, 99B and SGK9708-41, to mepiquat chloride （MC） applications (0 and 200 mg·kg-1) were stu

关键词： Bt protein Bt cotton heat stress gossypol condensed tannin

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Melatonin alleviates lipopolysaccharide-induced placental cellular stress response in mice

Melatonin alleviates lipopolysaccharide-induced placental ce...

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全国自由基医学与毒理学学术交流大会

作者：王华李玲赵梅陈远华张志辉张程姬艳丽孟秀红徐德祥 Department of Toxicology School of public healthAnhui Medical University

It has been demonstrated that melatonin protected mice from lipopolysaccharide(LPS)-induced fetal death and intra-uterine growth retardation. Nevertheless,its molecular mechanism remains *** the present study,we investigated the effects of melatonin on LPS-induced cellular stress in *** pregnant mice were administered with melatonin[5.0 mg/kg,intraperitoneal(i.p.)] 30 min before and 150 min after LPS(300μg/kg, i.p.) on gestational day *** stress, endoplasmic reticulum(ER) stress,hypoxic stress and heat stress in placenta were analyzed at 4 h after *** expected,maternal LPS administration resulted in placental GSH depletion and up-regulated the expression of placental antioxidative *** addition,LPS significantly increased the level of inducible nitric oxide synthase(iNOS) and enhanced the intensity of placental 3-nitrotyrosine(3-NT) *** ER stress,as determined by a decreased GRP78 expression,an obvious eIF2αand JNK phosphorylation,and an increased CHOP expression,were observed in placenta of pregnant mice injected with *** addition,LPS significantly increased mRNA level of placental HIF-1α,VEGF and ET-1,the markers of hypoxic *** oxygenase(HO)-1,a marker of heat stress,was also up-regulated in placenta of LPS-administered pregnant ***, LPS-induced placental oxidative stress,hypoxic stress and ER stress were significantly alleviated when pregnant mice were administered with melatonin,whereas melatonin had little effect on LPS-evoked placental HO-1 *** conclusion,maternally administered melatonin alleviates LPS-induced cellular stress in placenta. Melatonin may be used as pharmacological agents to protect the fetuses against LPS-induced intra-uterine fetal death and intra-uterine growth restriction.

关键词： oxidative stress endoplasmic reticulum stress hypoxic stress heat stress melatonin lipopolysaccharide placenta

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Impact of global climate change on the health,welfare and productivity of intensively housed livestock

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International Journal of Agricultural and Biological Engineering 2011年第2期4卷 1-22页

作者： Tadeusz Kuczynski Victoria Blanes-Vidal Baoming Li Richard S.Gates Irenilza de Alencar Nääs Daniella J.Moura Daniel Berckmans Thomas M.Banhazi Department of Environmental Engineering University of Zielona GoraZ.Szafrana 1Zielona Gora Poland Faculty of Engineering.University of Southern Denmark Niels Bohrs Alle 15230OdenseDenmark Department of Agricultural Structure and Bioenvironmental Engineering China Agricultural UniversityBeijing 100083China Agricultural and Biological Engineering 1304 West Pennsylvania AveUniversity of Illinois at Urbana-ChampaignUrbana IL 61801USA Agricultural Engineering College State University of CampinasCampinasSão PauloBrazil M3-BIORES Katholieke Universiteit LeuvenKasteelpark Arenberg 30Leuven Belgium National Centre for Engineering in Agriculture University of Southern QueenslandWest StToowoombaQLD 4350Australia

Major scientific studies have shown that global warming(*** average temperature of the Earth)is now a *** aims of this paper are to broadly review the underlining causes of global warming,the general effects of global warming on social and environmental systems and the specific effects of resulting from global warming phenomena severe fluctuations in weather patterns,particularly heat waves on livestock health,welfare and *** this article aims to summarise some common sense climate control methods and more importantly to highlight the required future research and development(R&D)work that is necessary to achieve a new level of building environment control capability,and thus ensure that the intensive livestock industries will be able to cope with the changed external *** the increasing temperatures on a global scale,the most direct effect of the high temperature on the animals is heat stress,which has been proven to have a variety of negative effects on animal health,welfare and *** potential measures could be taken in future to alleviate the increased heat *** of these measures are mere adaptations or improvements of current engineering ***,facing the complex challenges of global warming and particularly resulting from it the rapid increase of the number of consecutive days with significantly higher than average temperatures will probably require novel solutions,including new designs based on solid engineering judgment,development of new engineering standards and codes to guide designs,the exploration of new and superior building materials,the need for better energy management,and the development of substantially more“intelligent”control systems that will balance changing exterior disturbances,interior building loads and demands to the biological needs of the occupants of the structures.

关键词： livestock global climate change greenhouse effect animal welfare heat stress temperature cooling agricultural buildings

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Preservative Effect of Newbouldia laevis (Boundary Tree) Leaf Extract on Shelf-Life of Fresh Chicken Meat under Tropical Conditions

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Journal of Zoological Research 2020年第1期2卷 8-13页

作者： Oluwabukola Rashidat Popoola Ibukun Olukorede Popoola Olubunmi Olusola Department of Animal Science University of IbadanOyo StateNigeria Agricultural Research and Biometrics Department Thisavrous Pyrgos Int.ResourcesOyo StateNigeria

The shelf life of chicken meat has been rapidly reduced as a result of high environmental temperatures prevalent in the tropics which favored the activities of spoilage micro-organisms,and reactive oxygen species that function in oxidative *** laevis(***)possess valuable antioxidant and antibacterial ***,information on the effect of aqueous extracts of dry and wet leaves of *** on preservation of fresh chicken meat under tropical condition is scanty and thus,*** chicken meat(10kg weight)was obtained immediately after slaughter and were randomly allotted to three treatments(T1-control;T2-aqueous extract of wet leaves of ***;and T3-aqueous extract of dry leaves of ***)in a Randomized Complete Block *** plant,such as Newbouldia laevis with relatively high resistance to heat stress,possesses viable bioactive compounds that can lower the growth of spoilage micro-organisms and activities of reactive oxygen species on fresh chicken meat under tropical conditions for 48 ***,poultry farmers in developing nations with fluctuating power supply can adopt the quick meat shelf life enhancement technique,while commercial poultry farmers across the globe can embark on product fortification using extracts of Newbouldia laevis.

关键词： Aqueous extracts Antimicrobial Broiler chicken Newbouldia laevis Meat quality heat stress

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