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Inhibitory Activity of Prunus mume,Copitis chinensis and Crataegus pinnatifida on Vibrio harveyi and Its Biofilm

Agricultural Biotechnology 2017年第5期6卷 51-55页

作者： Yili ZHONG Danling GUO Huanying PANG Hao WU Yang HUANG Yucong HUANG Jufen TANG Jichang JIAN Fisheries College of Guangdong Ocean University Zhanjiang 524088China Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals Zhanjiang 524088China Key Laboratory of Diseases Controlling for Aquatic Economic Animals of Guangdong Higher Education Institutions Zhanjiang 524088China National Demonstration Center for Experimental Aquaculture Science Education Zhanjiang 524088China

[Objective] This study was conducted to determine the in-vitro inhibitory effects of Prunus mume,Coptis chinensis and Crataegus pinnatifida on Vibrio harveyi and its biofilm. [Method]The inhibitory zone diameters of the three Chinese herbal medicines against V. harveyi were determined by agar diffusion method; the minimal inhibitory concentration( MIC) and minimal bactericidal concentration( MBC) values of the three Chinese herbal medicines against V. harveyi were determined by doubling dilution method; and the effects of the three Chinese herbal medicines on the formation of V. harveyi biofilm were determined by methyl thiazolyl tetrazolium( MTT) method. [Result]The three Chinese herbal medicines all inhibited V. harveyi to different degrees. C. chinensis and C. pinnatifida and P. mume exhibited the inhibitory zone diameters of( 17. 62 ± 0. 04),( 20. 16 ± 0. 08) and( 30. 76 ± 0. 26) mm against V. harveyi,respectively. P. mume and C. pinnatifida had strong inhibitory effects on V. harveyi. The MIC and MBC values of P. mume against V. harveyi were 7. 812 5 mg/ml; the MIC and MBC values of C. pinnatifida against V. harveyi were 31. 25 mg/ml; and the MIC and MBC values of C. chinensis against V. harveyi were 62. 5 mg/ml. P. mume had the strongest antibacterial and bactericidal ability. The MIC values of C. pinnatifida,C. chinensis and P. mume against V. harveyi were 7. 81,7. 81 and 1. 96 mg/ml,respectively,i. e.,P. mume exhibited the lowest MIC. [Conclusion] P. mume,C. pinnatifida and C. chinensis all have inhibitory effects on V. harveyi and its biofilm,and P. mume has the strongest bactericidal ability.

关键词： Prunus mume Coptis chinensis Crataegus pinnatifida Vibrio harveyi Biofilm Inhibition

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Purification,characterization and production optimization of a vibriocin produced by mangrove associated vibrio parahaemolyticus

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Asian Pacific Journal of Tropical Biomedicine 2014年第4期4卷 253-261页

作者： Baskar Balakrishnan Jayappriyan Kothilmozhian Ranishree Sathish Thadikamala Prabakaran Panchatcharam Center for Bioenergy Cooperative researchLincoln University Marine Biotechnology Andaman and Nicobar Centre for Ocean Science and TechnologyNational Institute of Ocean Technology Department of Microbiology PRIST University

Objective:To identify a potential bacterium which produces antimicrobial peptide(vibriocin),and its purification,characterization and production optimization.The bacteria subjected in the study were isolated from a highly competitive ecological niche of mangrove ecosystem.Methods:The bacterium was characterized by phenotype besides 16S rRNA gene sequence analysis.The antibacterial activity was recognised by using agar well diffusion method.The vibriocin was purified using ammonium sulphate precipitation,butanol extraction,gel filtration chromatography,ion-exchange chromatography and subsequently,by HPLC.Molecular weight of the substance identified in SDS-PAGE.Production optimization performed according to Taguchi's mathematical model using 6 different nutritional parameters as variables.Results:The objective bacterium was identified as Vibrio parahaemolyticm.The vibriocin showed 18 KDa of molecular mass with mono peptide in nature and highest activity against pathogenic Vibrio harveyi.The peptide act stable in a wide range of pH,temperature.UV radiation,solvents and chemicals utilized.An overall^20%of vibriocin production was improved,and was noticed that NaCl and agitation speed played a vital role in secretion of vibriocin.Conclusion:The vibriocin identified here would he an effective alternative for chemically synthesized drugs for the management of Vibrio infections in mariculture industry.

关键词： Vibriocin Vibrio parahaemolyticus Vibrio harveyi Mangrove rhizosphere Antimicrobial peptide

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斜带石斑鱼烂尾病病原菌的分离与鉴定

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热带海洋学报 2010年第6期29卷 118-124页

作者：梅冰周永灿徐先栋王世峰谢珍玉海南大学海洋学院海南海口570228

2008年5月从海南省三亚红沙港与陵水新村湾网箱养殖的患"烂尾病"的斜带石斑鱼Epinephelus coioides中分离到3株优势菌株HS08001、HS08002和XC08001。人工感染试验表明,这3株菌株为斜带石斑鱼的致病菌。经菌株形态特征、培养特性和生理... 详细信息

2008年5月从海南省三亚红沙港与陵水新村湾网箱养殖的患"烂尾病"的斜带石斑鱼Epinephelus coioides中分离到3株优势菌株HS08001、HS08002和XC08001。人工感染试验表明,这3株菌株为斜带石斑鱼的致病菌。经菌株形态特征、培养特性和生理生化反应等表型测定以及16S rDNA测序分析,这3株细菌被鉴定为哈维氏弧菌Vibrio harveyi。其中,HS08001对斜带石斑鱼的半致死剂量为1.05×104CFU.g-1鱼体重。药敏试验表明,该菌具有较强的耐药性,在所检测的25中抗菌药物中,仅对庆大霉素、氯霉素和利福平高度敏感,而对阿莫西林等18种抗菌药物不敏感。

关键词：斜带石斑鱼Epinephelus coioides 哈维氏弧菌Vibrio harveyi 烂尾病 16SrDNA

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