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Isolation and characterization of halophilic bacteria and archaea from salt ponds in Hangu Saltworks, Tianjin, China

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Chinese Journal of Oceanology and Limnology 2015年第4期33卷 862-868页

作者：邓元告许高超隋丽英 Tianjin Key Laboratory of Marine Resources & Chemistry Tianjin University of Science & Technology TEDA

A total of 26 isolates were obtained from solar salt ponds of different salinities(100, 150, 200, and 250) in Hangu Saltworks Co. Ltd., Tianjin, China. Phylogenetic analysis of 16 SrRNA gene sequences indicated that five bacteria genera H alomonas, Salinicoccus, Oceanobacillus, Gracibacillus, and Salimicrobium and one archaea genera H alorubrum were present. The genus H alomonas was predominant with eight strains distributed in a salinity range of 100–200, followed by H alorubrum with six strains in salinity 250. Based on the genus and original sampling salinity, eight bacterial and two archaeal isolates were selected for further morphological, physiological, and biochemical characterization. All of the bacterial strains were moderately halophilic with the optimal salinity for growth being either 50 or 100, while two archaeal strains were extremely halophilic with an optimal growth salinity of 200. Additionally, we put forth strain SM.200-5 as a new candidate S alimicrobium species based on the phylogenic analysis of the 16 SrRNA gene sequence and its biochemical characteristics when compared with known related species.

关键词： halophilic bacteria halophilic archaea isolation salinity salt ponds

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Biogeographical note on Antarctic microforae: Endemism and cosmopolitanism

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Geoscience Frontiers 2013年第6期4卷 633-646页

作者： Waqar Azeem Jadoon Ryosuke Nakai Takeshi Naganuma Graduate School of Biosphere Science Hiroshima University1-4-4 KagamiyamaHigashi-HiroshimaHiroshima 739-8528Japan National Institute of Genetics 1111 YataMishimaShizuoka 411-8540Japan Research Fellow of the Japan Society for the Promotion of Science Chiyoda-kuTokyo 102-8471Japan

This study deals with the biogeography of Antarctic microflora （Antarctica acts as best model to study microbial biogeography） such as cyanobacteria and selected halophiles with special emphasis on Halomonas variabilis and Bacillus *** are known to be resistant not only to salt stress,but also to extreme temperature,pressure,and aridity and they are capable of surviving in harsh environments such as polar regions,deep-sea habitats,and *** microbes are known to be resistant to hostile environmental conditions,and are capable of surviving in harsh *** group has isolated 444 strains belonging to 28 genera of halophiles from various environments around the *** 16S rRNA gene sequences revealed that many of the isolated strains from geographically distant habitats having different environmental conditions,were closely related to each other,with some strains possessing 100％ identical *** possessing survival mechanism such as spore formation are usually *** genus Halomonas is represented by potentially endemic strains and the ubiquitous ***,while spore-forming *** showed cosmopolitan *** potentially endemic （moderate endemicity that is regional and/or continental distribution） strain was reported from Syowa station,East Antarctica,and Mario Zucchelli station,West Antarctica,which are geographically separated by 3000 ***,15 strains having 100％ similarity with *** were considered *** results of this work provide support for the middle-ground model that some microbes have moderate endemicity and others have cosmopolitan *** results will contribute to a greater understanding of microbial biogeography with special emphasis on Antarctica.

关键词： Biogeography Cyanobacteria halophilic bacteria 16S rRNA Molecular phylogeny

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A Study of the Characteristics of Microorganisms for Effective Degradation of Marine Oil Spills

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Petroleum Science 2006年第1期3卷 61-67页

作者： Zhang Zhongzhi Wei Xiaofang Zong Chang Yu Hongwen Li Chengjian He Ruibing Faculty of Chemistry ＆ Chemical Engineering China University of Petroleum Beijing 102249 China Bohai Oil Technical Service Company China National Offshore Oil Corp.Tanggu Hebei 065000 China

Four microorganism strains were isolated from coastal petroleum-polluted soil and sand samples of Bohai Sea oilfield; they were found to degrade marine oil spills effectively. The experimental results show that the degradation efficiency of crude oil with these four strains （XT-4, SZ-1-25, B-4-9, BS-3-12） is 95.97%, 96.01%, 97.99% and 98.99%, respectively, in their optimum conditions. The characteristics of bacterial biodegradation are investigated. The simulation biotreatment of oil-contaminated beach sand, with an initial oil content of 5,664mg/kg-dry-sand, shows that the residual oil content is 2,700 mg/kg-dry-sand and 2,679 mg/kg-dry-sand after 170 days＇ treatment with two bacteria （B-4-9, BS-3- 12）, respectively.

关键词： Petroleum pollution halophilic bacteria biodegradation simulation biotreatment of beach sand

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盐单胞菌ZY-1合成的PHA材料的发酵条件优化及性能分析

盐单胞菌ZY-1合成的PHA材料的发酵条件优化及性能分析

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作者： Baffoe Dennis Kingsley 东北林业大学

学位级别：硕士

聚羟基链烷酸酯(PHA)具有广泛的物理性能,可替代多种化学原料,因此在多个领域中已得到应用。然而,PHA的生产成本较高,是石油基塑料的4-5倍,严重影响了 PHA的广泛应用。因此,需要一种低成本并提高PHA产量的装置。根据嗜盐菌的特点,可以... 详细信息

聚羟基链烷酸酯(PHA)具有广泛的物理性能,可替代多种化学原料,因此在多个领域中已得到应用。然而,PHA的生产成本较高,是石油基塑料的4-5倍,严重影响了 PHA的广泛应用。因此,需要一种低成本并提高PHA产量的装置。根据嗜盐菌的特点,可以开发出非灭菌连续发酵装置,该装置对于降低PHA的生产成本,促进PHA产业的可持续发展具有重要意义。从黑龙江省大庆地区盐碱土中筛选出高产PHA的嗜盐菌菌株,通过苏丹黑和异染颗粒染色法确定PHA的积累,通过形态观察和分子生物学技术将该菌株鉴定为Halomonassp.并命名为 ZY-1。初步研究了营养培养和发酵条件对菌株ZY-1合成PHA的影响以及生长规律。结果表明,最好的碳源是葡萄糖;NH4Cl的最佳浓度为1 g/L;KH2PO4的最佳浓度为1.5 g/L;菌株ZY-1在pH=9时合成PHA的能力最强,为2.8g/L。在此条件下,采用两种分批补料的方法来提高PHA的产量,第一种是将葡萄糖浓度控制在20-50 g/L,PHA最高产量为5.76 g/L,占细胞干重的48%,是摇瓶发酵产PHA的2.05倍。第二种策略是通过单个脉冲将葡萄糖浓度增加到100g/L的高浓度,PHA的最终产量为4.9g/L,占细胞干重的42%,是摇瓶发酵PHA产量的1.75倍。使用红外光谱(FTIR)对由乙酸钠(2C),葡萄糖(6C)和油酸(18C)制成的三种不同材料与PHB标准样品进行分析比较。结果表明,三种材料的特征吸收峰与PHB标准样品的特征吸收峰相同,表明菌株ZY-1使用不同碳源为材料合成的产物PHA。通过X射线衍射(XRD),扫描电子显微镜(SEM)和差示扫描量热法(DSC)分析了三种PHA材料的物理性能。结果表明,碳源的碳链越长,合成的PHA的韧性,透明度和成膜性越好,且结晶度越低。三种PHA的熔融温度相似,在160至170℃之间,具有出色的热稳定性。为了探索PHA的降解,本实验分析了 PHA在不同环境中的降解。结果表明,土壤中PHA的失重率大于PBS缓冲液。在不同的pH溶液中,PHA的失重率为:pH 12.0>pH 9.0>pH 3.0>pH 7.0。总言之,PHA材料具有良好的生物降解性。为了开发用于PHA合成的低成本碳源,文章研究了稻草和油酸中PHA的积累。结果表明,在秸秆培养基中发酵84小时,PHA的最高产量为0.0805 g/L,合成的PHA为棕色粉末。在秸秆油酸介质中,当秸秆上清液与油酸的体积比为45:1时,PHA的产量达到1.57 g/L。PHA相对透明且坚韧,可合成更好的生物膜。

关键词： halophilic bacteria PHA Fermentation optimization Material properties Economic carbon source

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