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half smooth tongue sole(Cynoglossus semilaevis) Under Low Salinity Stress Can Change Hepatic igf2 Expression Through DNA Methylation

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Journal of Ocean University of China 2020年第1期19卷 171-182页

作者： LI Siping HE Feng WEN Haishen SI Yufeng LIU Mingyuan HUANG Yajuan WU Shuxian Fishery College the Key Laboratory of Mariculture of Ministry of EducationOcean University of ChinaQingdao 266003China

Salinity is a crucial environmental stress that severely affects fish growth and *** environmental stress,DNA methylation plays an important role in gene expression and genome *** better understand the epigenetic regulation mechanism of igf2 under low salinity stress,we analyzed the DNA methylation at 5’UTR,exon1,intron1,and exon2,and analyzed the relationship of DNA methylation with mRNA abundance as well as the special single CpG sites methylation patterns of igf2 in the liver of half smooth tongue sole under low salinity(15)for 0,7,and 60 *** exposed to low salinity,DNA methylation at 5’UTR and exon2 remained stable,while it was up-regulated firstly and then down-regulated at exon1 and *** single CpG sites of igf2 associated with low salinity,and most of these sites with significantly changed methylation levels(Phalf smooth tongue sole liver can regulate the expression of igf2 through methylation of CpG sites in intron1.

关键词： salinity hepatic igf2 DNA methylation gene expression half smooth tongue sole

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Development and characterization of a cell line from the embryos of half smooth tongue sole (Cynoglossus semilaevis)

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Acta Oceanologica Sinica 2010年第2期29卷 81-87页

作者： SHA Zhenxia REN Guocheng WANG Xianli WANG Na CHEN Songlin Key Laboratory of Sustainable Development of Marine Fisheries Ministry of Agriculture Yellow Sea Fisheries Research Institute Chinese Academy of Fisheries Sciences Qingdao 266071 China College of Biology Shandong Normal University Jinan 250014 China College of Marine Life Ocean University of China Qingdao 266003 China

A new cell line, CSEC, has been successfully established from embryos at gastrula stage of a cultured marine fish, half smooth tongue sole （Cynoglossus sernilaevis）. CSEC cells grow actively and stably more than 50 passages for over 200 d in DMEM medium supplemented with 15% FBS （fetal bovine serum）, 2.5 ng/cm^3 bFGF （basic fibroblast growth factor）, 1 ng/cm^3 LIF （leukemia inhibitory factor） and 50 mmol/dm^3 2-ME （2-mecaptoethanol）. The cells grew well in the temperature range of 24-30 ℃ and the optimal growth temperature was 24 ℃. FBS and bFGF concentrations are the two key components for CSEC cells proliferation. Chromosome analysis reveals that CSEC cells have a normal diploid karyotype with 2n=42t. The significant fluorescent signals were observed in CSEC after transfection with the GFP reporter gene, suggesting that the CSEC cell line can be used as a useful tool for transgenic and genetic manipulation studies. CSEC cells showed the cytopathic effect （CPE） after infection with lymphosystis disease virus （LCDV） in 2 d. Moreover, the LCDV particles can be observed in the cytoplasm of virus-infected cells by electron microscopy. It suggests that CSEC could be potentially used for the study of aquatic virus.

关键词： embryonic cell line half smooth tongue sole karyotype GFP LCDV CSEC

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