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Aberrantly Methylated MGMT,hmlh1.and hMSH2 in Tumor and Serum DNA of Gliomas Patients

Chinese Journal of Clinical Oncology 2009年第1期6卷 42-46页

作者： Changqing Zheng Shouping Ji Feng Gong Anming Li Junli Tai Subuo Li Yingli Wang Hongyu Chang Hongwei Gao Yangpei Zhang Department of Biochemistry and Molecular Biology Institute of Transfusion Medicine Academy of Military Medical Sciences Beijing 100850 China No.1 Affiliated Hospital PLA General Hospital Beijing 100037 China.

OBJECTIVE This study is to investigate the prevalence ofpromoter CpG island methylation of O^6-methylguananine-DNAmethyltransferase (MGMT), mismatch repair genes (hmlh1.andhMSH2) in both tumor and serum samples of *** Methylation-specific PCR (MSP) was employed todetect promoter CpG island methylation of the MGMT, hmlh1.nd hMSH2 genes in 39 samples taken from surgery and 32samples of pretreatment serum all from the patients with *** Promoter CpG island methylation of MGMT, hmlh1.nd hMSH2 was detected and the results were 46.2%, 10.3% and20.5%, respectively in tumor DNA of the cases with gliomas,and 40.6%, 9.4% and 18.8%, respectively in serum DNA of thecases. The methylation pattern in primary tumor and serum wasfound to be concordant in matched tissue and serum samplesof 21 patients. In the cases with positive result of methylationfor MGMT, hmlh1.and hMSH2 in tumor tissues, the results ofdetection for those in the paired serum sample were 77.8% (7/9),66.7% (2/3) and 75.0 % (3/4), respectively. False positive resultswere not obtained in any of the patients who did not exhibitmethylation. No association was found between the promotermethylation of MGMT, hmlh1. and hMSH2 genes in primarygliomas and gender, age, localization, grade of malignant or *** Promoter CpG island methylation is a frequentevent in gliomagenesis. Methylation analysis appears to bea promising predictive factor of the prognosis for the gliomapatients treated with alkylating drugs and a noninvasive tumormarker in serum DNA.

关键词： gliomas promoter CpG island hypermethylation DNA repair MGMT hmlh1.

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