检索结果-南通市图书馆

Intracolonial differences in gut bacterial community between worker and soldier castes of Coptotermes formosanus

Insect Science 2012年第1期19卷 86-95页

作者： Hui Xiang Lei Xie Jun Zhang Yan-Hua Long Ning Liu Yong-Ping Huang Qian Wang Institute of Plant Physiology and Ecology Shanghai Institutes for Biological SciencesChinese Academy of SciencesShanghaiChina

The establishment of symbiotic relationships with intestinal microorganisms enables termites to thrive on recalcitrant substrates such as cellulose and wood. A termite colony is composed of several different castes which have distinct feeding habits. The soldiers, for example, cannot feed by themselves and depend on workers, who feed them with digested or semi-digested foods. To investigate the influence of feeding habits on the bacterial symbionts, a comparative study of gut bacteria between worker and soldier castes of the termite Coptotermesformosanus was conducted. The bacterial communities of both castes were investigated using denaturing gradient gel electrophoresis （DGGE） and clonal analysis of 16S ribosomal DNA （rDNA）. Both methods indicated Bacteroidetes was the common predominant group; the common dominant phylotype was affiliated with a reported uncultured Bacteroidetes phylotype （BCfl-03）. There were significant differ- ences in Bacteroidetes and Spirochaetes between two castes. Compared to the gut bacteria of workers, those of soldiers were lower in abundance and diversity of Bacteroidetes and slightly higher in Spirochaetes. Two phylotypes （W8, W11） affiliated to Bacteroidetes and two （W26, W29） affiliated to Spirochaetes were exclusively found in the DGGE profile of the worker caste. Bacteroidetes are assumed to be involved in fermentation of sugars and nitrogenous compounds as well as degradation of uric acid. Spirochaetes are supposed to aid in the functions of acetogenesis and N2-fixation. The different feeding habits between workers and soldiers of C. formosanus may explain the observed differences in the gut bacterial community.

关键词： Coptotermesformosanus gut bacteria community 16S rDNA

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

bacterial community structure in Apis florea larvae analyzed by denaturing gradient gel electrophoresis and 16S rRNA gene sequencing

引用

Insect Science 2015年第5期22卷 606-618页

作者： Prakaimuk Saraithong Yihong Li Kanokporn Saenphet Zhou Chen Panuwan Chantawannaku Department of Biology Faculty of Science Chiang Mai University Chiang Mai 50200 Thailand Department of Basic Science and Craniofacial Biology New York University College of Dentistry New York 10010 USA and Materials Science Research Center Faculty of Science Chiang Mai University Chiang Mai 50200 Thailand

This study characterizes the colonization and composition of bacterial flora in dwarf Asian honeybee （Apisflorea） larvae and compares bacterial diversity and distribu- tion among different sampling locations. A. florea larvae were collected from 3 locations in Chiang Mai province, Thailand. bacterial DNA was extracted from each larva using the phenol-chloroform method. Denaturing gradient gel electrophoresis was performed, and the dominant bands were excised from the gels, cloned, and sequenced for bacterial species identification. The result revealed similarities of bacterial community profiles in each individual colony, but differences between colonies from the same and different locations. A. florea larvae harbor bacteria belonging to 2 phyla （Firmicutes and Proteobac- teria）, 5 classes （Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Bacilli, and Clostridia）, 6 genera （Clostridium, Gilliamella, Melissococcus, Lactobacillus, Saccha- ribacter, and Snodgrassella）, and an unknown genus from uncultured bacterial species. The classes with the highest abundance of bacteria were Alphaproteobacteria （34%）, Bacilli （25%）, Betaproteobacteria （11%）, Gammaproteobacteria （10%）, and Clostridia （8%）, re- spectively. Similarly, uncultured bacterial species were identified （12%）. Environmental bacterial species, such as Saccharibacterfloricola, were also found. This is the first study in which sequences closely related to Melissococcus plutonius, the causal pathogen re- sponsible for European foulbrood, have been identified in Thai A. florea larvae.

关键词： A. florea DGGE gut bacteria community larvae 16S rRNA gene

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：