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ZmOST1 mediates abscisic acid regulation of guard cell ion channels and drought stress responses

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Journal of Integrative Plant Biology 2019年第4期61卷 478-491页

作者： Qiqi Wu Mei Wang Jianlin Shen Donghua Chen Yu Zheng Wei Zhang Key Laboratory of Plant Development and Environmental Adaption Biology Ministry of Education School of Life Science Shandong University

The phytohormone abscisic acid(ABA) is an important mediator in the drought response, participating in, among other processes, stomatal movements. In Arabidopsis thaliana, the serine/threonine protein kinase,OST1, regulates this response, but the function of its maize homolog has yet to be established. Here, we isolated ZmOST1 and show that its encoded protein indeed acts to regulate guard cell movement. ZmOST1 was ubiquitously expressed throughout the plant, being highly expressed in guard cells, and inducible both by exogenous ABA and water stress. Transient expression of a ZmOST1-GFP fusion protein, in maize mesophyll protoplasts, indicated its subcellular localization in the cytoplasm and nucleus. A Zmost1 loss-of-function mutant exhibited reduced sensitivity to ABA-activated slow anion channels in maize guard cells, and reduced drought tolerance. Constitutive expression of ZmOST1, in an A. thaliana ost1-1 mutant rescued the phenotype with respect both to the sensitivity of guard cell slow anion currents to ABA treatment and stomatal closure. Our findings indicate a positive regulatory role for ZmOST1 in guard cell ABA signaling and drought response in maize plants.

关键词： ZmOST1 abscisic acid regulation guard cell

Effects of La^(3+) on inward K^+ channels at plasma membr in guard cells

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Science China Chemistry 2005年第2期48卷 143-147页

作者： XUE Shaowu YANG Pin Institute of Molecular Science Chemical Biology and Molecular Engineering Laboratory of Ministry of Education Shanxi University Taiyuan 030006 China Institute of Molecular Science Chemical Biology and Molecular Engineering Laboratory of Ministry of Education Shanxi University Taiyuan 030006 China

The effects of La3+ on inward K+ channels at plasma membrane in vicia guard cells are investigated using the whole-cell patch-clamp recording mode. It is shown that La3+ on both sides of plasma membrane blocks inward K+ currents in a concentration- dependent manner, indicating that La3+ binding sites may exist on both sides of plasma membrane in guard cells in vicia. The dose response is fitted by the Michaelis-Menten relation characterized by an inhibitor constant Ki of 2.56±0.25 μmol·L?1 (outside membrane) and (1.18±0.11)×10?15 mol·L?1 (inside membrane). Intracellular La3+ has much stronger inhibitory effect on inward K+ currents than ex- tracellular La3+ does, suggesting there may exist stronger binding sites inside membrane than outside membrane. Since ion channel activities of guard cells directly affect plant stomatal movement and water status, our results imply that rare earth elements might have potential practical values in regulating plant water status and strengthening plant drought endurance.

关键词： whole-cell patch-clamp, guard cell, stomatal movements, inward K+ channels, La3+.

Regulating role of acetylcholine and its antagonists in inward rectified K^+ channels from guard cell protoplasts of Vicia faba

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Science China(Life Sciences) 2000年第2期43卷 217-224页

作者：冷强花宝光郭玉海娄成后 1. Plant Physiology Laboratory Chinese Agricultural University 100094 Beijing China 2. Plant Physiology and Biochemistry Laboratory Beijing Agricultural College 102206 Beijing China

The inward rectified potassium current of Vicia faba guard cell protoplasts treated with acetylcholine (ACh) or the antagonists of its receptors were recorded by employing the patch clamp technique. The results show that ACh at lower concentrations increases the inward K+ current, in contrast, ACh at higher concentrations inhibits it. Treated with d-Tubocurarine (d-Tub), an antagonist of the nicotine ACh receptor (nAChR) inhibits the inward K+ current by 30%. Treated with atropine (Atr), an antagonist of the muscarine (Mus) ACh receptor (mAChR) also inhibits it by 36%. However, if guard cell protoplasts are treated with d-Tub and Atr together, the inward K+ current is inhibited by 60%-75%. Tetraethylammonium chloride (TEA), a strong inhibitor of K+ channels has no effect on the inward K+ current regulated by ACh, suggesting that there are inward K+ channels modulated by AChRs on the membrane of the guard cell protoplasts. These data demonstrate an ACh-regulated mechanism for stomatal movement.

关键词： acetylcholine acetylcholine receptor inward K+ current guard cell.

Effects of La^(3+) and Eu^(3+) on outward potassium channels in Vicia guard cells

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Science China Chemistry 2009年第11期52卷 1821-1824页

作者： XUE ShaoWu & YANG Pin Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education,Institute of Molecular Science,Shanxi University,Taiyuan 030006,China 1. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education Institute of Molecular Science Shanxi University Taiyuan 030006 China

Effects of La3+ and Eu3+ on outward potassium channels(K+out) in Vicia guard cells have been studied by patch clamping *** La3+ inhibited K+out currents with a half-inhibition concentration(IC50) of 81 μmol·***,intracellular La3+ activated K+out currents at a free concentration of 1.13 × 10-14 mol·L-1,and inhibited K+out currents at a free concentration of 5.86 × 10-14 mol·*** Eu3+ also activated K+out currents at concentrations of 10 μmol·L-1 and 50 μmol·L-1,and inhibited K+out currents at concentrations of more than 1 mmol·*** effects of La3+ and Eu3+ on K+out currents may contribute to regulation of the plant water status,which may be one of the mechanisms of the biological effect of rare earth elements.

关键词： rare earth elements stomata guard cell outward potassium channel

MAP kinase specifically mediates the ABA-induced H_2O_2 generation in guard cells of Vicia faba L.

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Chinese Science Bulletin 2003年第18期48卷 1919-1926页

作者： JIANG Jing1,2, AN Guoyong1, WANG Pengcheng1, WANG Pengtao1, HAN Jinfeng2, JIA Yanbin1 & SONG Chunpeng1 1. College of Life Sciences, Henan University, Kaifeng 475001, China 2. College of Agriculture Science, Henan Agriculture University, Zheng-zhou 450001, China Correspondence should be addressed to Song Chunpeng (e-mail: songcp @henu. ***) College of Life Sciences Henan University Kaifeng China College of Agriculture Science Henan Agriculture University Zhengzhou China

Mitogen-activated protein (MAP) kinase is involved in ABA- or H2O2-signaling, and H2O2 acts as inter-mediate in the downstream of ABA signal transduction pathway, which has recently emerged as a secondary mes-senger of ABA signaling. Using an epidermal strip bioassay and laser scanning confocal microscope, we provided the first evidence that MAP kinase plays an important role in H2O2 signal initial, amplification and specific targeting in response to stimuli in guard cells. ABA- or H2O2-induced Vicia faba stomatal closure was inhibited or reversed by the specific inhibitor PD98059 of MEK1/2; the guard cells were pre-incubated or -microinjected by 10 mmol·L-1 PD98059, ABA could not enhance the fluorescence intensity of H2O2 probe dichlorofluorescein (DCF). Meanwhile, after ABA induced the H2O2 accumulation in guard cells, the exogenous or intracellular PD98059 could reduce the DCF fluorescence intensity. Most interestingly, on the contrary to ABA, the DCF fluorescence intensity of guard cells treated by 100 mmol·L-1 salicylic acid (SA) was not down-regulated by PD98059, yet PD98059 did not regulate the stomatal move-ment being induced by light, dark or salicylic acid. These results suggest that MEK1/2 could mediate stomatal closure by abolishing the ABA-induced H2O2 generation/accumula- tion in the specific manner.

关键词： MAP kinase PD98059 ABA H2O2 guard cells.

Localization of muscarinic acetylcholine receptor in plant guard cells

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Chinese Science Bulletin 2001年第7期46卷 586-588,618页

作者： Fanxia Meng Xin Liu Shuqiu Zhang Chenghou Lou China Agr Univ Coll Biol Sci Minist Agr Key Lab Plant Physiol & Biochem Beijing 100094 Peoples R China

Acetylcholine (ACh), as an important neuro-transmitter in animals, also plays a significant role in various kinds of physiological functions in plants. But relatively little is known about its receptors in plants. A green fluorescence BODIPY FL-labeled ABT, which is a high affinity ligand of muscarinic acetylcholine receptor (mAChR), was used to localize mAChR in plant guard cells. In Vicia faba L. and Pisum sativum L., mAChR was found both on the plasma membrane of guard cells. mAChR may also be distributed on guard cell chloroplast membrane of Vicia faba L. The evidence that mAChR localizes in the guard cells provides a new possible signal transduction pathway in ACh mediated stomata movement.

关键词： guard cell mAChR fluorescent localization Vicia faba L. Pisum sativum L.

Identification of integrin-like in guard cells of Vicia faba

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Chinese Science Bulletin 2001年第13期46卷 1100-1103页

作者： ZHANG Aihua HUANG Rongfeng WANG Xuechen YUAN Ming College of Biological Sciences China Agricultural UniversityBeijing100094China Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China Key Lab of Plant Physiology&Biochemistry The Ministry of AgricultureBeijing 100094China

We addressed the existence and localization of integrin-like in guard cells of Vicia faba by using a probe of polyclonal antibody against the human integrin (αvβ3/β5)-Western blot results showed that three integrins-like of about 47.3, 43.7 and 41.1 ku were detected from the preparation of membrane fragments of purified guard cell protoplasts. Further research with immunofluorescent scanning microscopy indicated that those integrins-like were localized on plasma membrane of guard cells, most nearing the dorsal wall, which is consistent with the reception of signals from epidermal cells to guard cells. Thus our results indicate, for the first time, that integrins-like are present at guard cell plasma membrane of Vicia faba.

关键词： integrins-like guard cells Viciafaba signal transduction.

WeiTsing:A guard of the stele

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Molecular Plant 2023年第8期16卷 1237-1239页

作者： Muhammad Arslan Mahmood Rubab Zahra Naqvi Shahid Mansoor Plant Sciences Division Research School of BiologyThe Australian National UniversityCanberraACT2601Australia National Institute for Biotechnology and Genetic Engineering(NIBGE)College of Pakistan Institute of Engineering and Applied Science(PIEAS) FaisalabadPakistan international Center for Chemical and Biological Sciences(ICCBS) University ofKarachiKarachiPakistan

Soil-borne pathogens,e.g.,Plasmodiophora brassicae,causes devastating clubroot disease,and existing identified resistance(R)genes has been broken down with the co-evolution of pathogen,threatening the future of cruciferous ***,there is a dire need to identify new R genes for developing clubroot-resistant ***,Wang et al.,2023 have reported a new R gene,WeiTsing,that confers complete resistance to Plasmodiophora brassicae,providing an opportunity to deliver broad-spectrum,durable clubroot-resistant crop varieties.

关键词： al. guard resistance

A Case of New Variant COVID-19 First Emerging in South Africa Detected in a Security guard at the Isolation Point—Shenzhen,China,January 23,2021

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China CDC weekly 2021年第10期3卷 218-220页

作者： Jun Meng Shujiang Mei Long Chen Chunli Wu Shisong Fang Bo Peng Dongfeng Kong Xiaomin Zhang Linghong Xiong Yalan Huang Qiongcheng Chen Renli Zhang Yaqing He Department of Pathogenic Biology Shenzhen Center for Disease Control and PreventionShenzhenGuangdongChina Department of Communicable Diseases Control and Prevention Shenzhen Center for Disease Control and PreventionShenzhenGuangdongChina.

On January 22,2021,a 34-year-old male,who served as a security guard at the isolation point in Shenzhen,tested positive for coronavirus disease 2019(COVID-19)during the every-three-day routine *** the nasopharyngeal swab was further confirmed as positive for COVID-19 by Shenzhen CDC,the patient was transferred to the Third People’s Hospital of Shenzhen and was diagnosed as a COVID-19 asymptomatic *** January 25,2021,Shenzhen CDC identified the 20H/501.Y.V2(B.1.351)variant,which was a variant of COVID-19 virus first emerging in the South Africa.

关键词： routine nasopharyngeal guard