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Effect of recombinant growth hormone on expression of growth hormone receptor, insulin-like growth factor mRNA and serum level of leptin in growing pigs

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Science China(Life Sciences) 2003年第2期46卷 205-211页

作者：胥清富赵志辉倪迎冬赵茹茜陈杰 Laboratory of Animal Physiology&Biochemistry College of Animal MedicineNanjing Agricultural UniversityNanjing 210095China

Sixteen Large White ?Landrace castrated male pigs were allotted into treatment and control group. The treatment group was injected intramuscularly with recombinant porcine growth hormone (rpGH, 4 mg·d-1) and the control group with vehicle for 28 days. Animals were slaugh-tered 4 h after final injection for liver, longissimus dorsi (LD) muscle and blood sampling. Serum concentration of insulin-like growth factor 1 (IGF-I) and leptin were determined by RIA. The total RNA was extracted from tissues to measure the abundance of growth hormone receptor (GHR), IGF-I mRNA by RT-PCR with 18S rRNA internal standard. Results showed that rpGH enhanced the average daily weight gain by 26.1% (P 0.05) and IGF-I mRNA (P > 0.05) in LD between GH treated and control group was found. These results suggest that rpGH can up-regulate hepatic GHR and IGF-I gene expression and improve animal growth. However the effect of rpGH on GHR and IGF-I gene ex-pression are tissue-specific.

关键词： pigs growth hormone insulin-like growth factor growth hormone receptor

维普期刊数据库

Identification and Characterization of GH receptor and Serum GH-binding Protein in Chinese Sturgeon (Acipenser sinensis)

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Acta Biochimica et Biophysica Sinica 2004年第12期36卷 811-816页

作者： Zhi-Yong LIAO1,2 and Shang-Quan ZHU2* 1College of Life Sciences, Jiangxi Normal University, Nanchang 330027, China 2Key Laboratory of Proteomics Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China College of Life Sciences Jiangxi Normal UniversityNanchang 330027 China Key Laboratory of Proteomics Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of SciencesShanghai 200031 China

Chinese sturgeon, a kind of cartilage ganoid, has a history of over one billion years and it is called the living fossil of aquatic biology since it keeps some evolutionary trace. Here, we characterized the growth hormone receptor (GHR) and serum growth hormone binding protein (GHBP) of Chinese sturgeon. It was shown that GHR was expressed in various tissues, mainly in hepatic, kidney and intestine tissues. GHR on the hepatic membrane has high and specific affinity for bream GH (brGH) and Scatchard analysis of the binding data showed a single class of high affinity binding site with an association constant Ka of 3.1×109 M–1. A specific band around 94 kD was detected by SDS-PAGE in cross-linking studies of membrane receptors. After incubation of Chinese sturgeon serum with 125I-brGH, a 125I-brGH-GHBP complex was identified by Sephadex G-75, indicating that in the serum exists GHBP specially binding to brGH.

关键词： Chinese sturgeon growth hormone receptor growth hormone binding protein ligand binding

Effect of sericin on diabetic hippocampal growth hormone/insulin-like growth factor 1 axis

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Neural Regeneration Research 2013年第19期8卷 1756-1764页

作者： Zhihong Chen Songhe Yang Yaqiang He Chengjun Song Yongping Liu Department of Human Anatomy Chengde Medical College Department of Biochemistry Chengde Medical College

Previous studies have shown that sericin extracted from silk cocoon significantly reduces blood glucose levels and protects the nervous system against diabetes mellitus. In this study, a rat type 2 diabetes mellitus model was established by intraperitoneal injection of 25 mg/kg streptozotocin for 3 successive days, following which the rats were treated with sericin for 35 days. After treatment, the blood glucose levels of the diabetic rats decreased significantly, the growth hormone level in serum and its expression in the hippocampus decreased significantly, while the insulin-like growth factor-1 level in serum and insulin-like growth factor-1 and growth hormone receptor expression in the hippocampus increased significantly. The experimental findings indicate that sericin improves disorders of the growth hormone/insulin-like growth factor 1 axis to alleviate hippocampal damage in diabetic rats.

关键词： neural regeneration traditional Chinese medicine sericin type 2 diabetes mellitus hippocampus growth hormone insulin-like growth factor 1 growth hormone receptor growth hormone/insulin-likegrowth factor 1 axis streptozotocin blood glucose western blot assay reverse transcription-PCR grants-supported paper neuroregeneration

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The effects of growth hormone on therapy resistance in cancer

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Cancer Drug Resistance 2019年第3期2卷 827-846页

作者： Reetobrata Basu John J.Kopchick Ohio University Heritage College of Osteopathic Medicine(OU-HCOM) Ohio UniversityAthensOH 45701USA Edison Biotechnology Institute Ohio UniversityAthensOH 45701USA.

Pituitary derived and peripherally produced growth hormone(GH)is a crucial mediator of longitudinal growth,organ development,metabolic regulation with tissue specific,sex specific,and age-dependent *** and its cognate receptor(GHR)are expressed in several forms of cancer and have been validated as an anti-cancer target through a large body of in vitro,in vivo and epidemiological ***,the underlying molecular mechanisms of GH action in cancer prognosis and therapeutic response had been sparse until *** review assimilates the critical details of GH-GHR mediated therapy resistance across different cancer types,distilling the therapeutic implications based on our current understanding of these effects.

关键词： growth hormone receptor growth hormone therapy resistance chemoresistance