检索结果-南通市图书馆

Cloning, characterization and expression analysis of Ctenopharyngodon idella VDAC1 that is up-regulated in response to grass carp reovirus infection revealed by proteomic analysis

Cloning, characterization and expression analysis of Ctenoph...

引用

中国水产学会鱼病专业委员会2013年学术研讨会

作者： Xiaobao Shen Tu Wang Dan Xu Liqun Lu National pathogen collection center for aquatic animals Shanghai Ocean University

Voltage-dependent anino channel(VDAC),located in the mitochondrial outermembrane,was known as mitochondrial porin and play a central role in regulating cell life *** this report,the voltage-dependent anion channel protein 1 from grass carpCtenopharyngodon idella(designated as CiVDAC1)was identified to be up-regulated by GCRVinfection through two-dimensional gel electrophoresis and protein analysis of infected CIK *** full-length cDNA of CiVDAC1 is 995bp with an open reading frame of 852bp encoding a 283amino acid *** complete ORF of grass carp VDAC1 demonstrated a high identity withwell characterized mammalian homologues through phylogenic *** deduced CiVDAC1contains anα-helix at the amino terminal,19 membrane spanningβ-strands and one eukaryoticmitochondrial porin signature *** tropism analysis indicated that CiVDAC1 is abundantin muscle,heart,skin,swim ladder,trunk kidney,*** expression profilesindicated that CiVDAC1 gene was up-regulated upon viral challenge in a manner that was similarto Mx2 gene,the marker gene for the activation of innate antiviral *** expressionpattern of CiVDAC1 gene was observed in CIK cells stimulated with Poly(I:C),as well as kidneytissue of grass carp challenged with GCRV in ***1 silencing in CIK cells displayed noimpact on vprogeny virus *** interpret the role of other VDAC proteins in viralpathogenesis,VDAC2 was further characterized and demonstrated to respond to GCRV challengein a similar way as ***1 protein was thus determined to be a virus-induced factor ingrass carp cells with no detectable effect on GCRV replication.

关键词： grass carp reovirus CiVDAC1 CiVDAC2 Mx2 Poly(I:C)

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Proteomic identification and characterization of Ctenopharyngodon idella tumor necrosis factor receptor-associated protein 1（CiTrap1）: an anti-apoptosis factor upregulated by grass carp reovirus infection

Proteomic identification and characterization of Ctenopharyn...

引用

2014年中国水产学会学术年会

作者： Yan Li Yanan Zhang Tu Wang Patarida Podok Dan Xu Liqun Lu Key Laboratory of Freshwater Fishery Germplasm Resources Ministry of Agriculture Shanghai Ocean University

Human tumor necrosis factor receptor-associated protein 1（Trap1） is a mitochondrial protein identical to heat shock protein 75（HSP75） that plays an important role in protecting cells from oxidative stress and apoptosis. In this study, grass carp（Ctenopharyngodon idella） tumor necrosis factor receptor-associated protein 1（designated as Ci Trap1） was identified through two-dimensional electrophoresis（2-DE） analysis and its patterns of expression was investigated in grass carp kidney（CIK） cells infected with grass carp reovirus（GCRV）. The full length c DNA of Ci Trap1 was cloned and sequenced revealing it to contain an opening reading frame of 2157 bp that encoded a peptide of 718 amino acids. Phylogenetic analyses indicated that the Ci Trap1 shared 87 % homology with the zebrafish（Danio rerio）. The transcriptional level of Ci Trap1 in CIK cells was upregulated 24 h after virus infection with similar expression patterns of the Ci Trap1 gene observed after poly（I:C） stimulation. At later stages of infection the expression level was downregulated. Following virus infection the expression levels of grass carp PTEN-induced putative kinase 1（PINK1） and Sorcin, whose human homologues interact with Trap1 during anti-apoptosis, exhibited similar expression patterns to that of Ci Trap1. Typical characteristics of apoptosis, including the formation of an apoptotic body and DNA fragmentation, were observed in CIK cells infected with GCRV by DAPI staining, DNA ladder electrophoresis and TUNEL assay. Although the m RNA expression level of caspase-3, as well as the Bax/Bcl-2 ratio, was upregulated during GCRV infection, the apoptosis was seemed to be self-limiting by our apoptosis assay. The results of this study suggest that Ci Trap1 is involved in the host’s innate immune response to viral infection, possibly through protecting infected cells from apoptosis.

关键词： grass carp(Ctenopharyngodon idella) proteomic analysis tumor necrosis factor receptor-associated protein 1 apoptosis grass carp reovirus

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Using yeast two-hybrid system to screen interesting cellular proteins with grass carp reovirus no-structual proteion 26

Using yeast two-hybrid system to screen interesting cellular...

引用

中国水产学会鱼病专业委员会2013年学术研讨会

作者： Hao Wang Xiaobao Shen Dan Xu Liqun Lu National pathogen collection center for aquatic animals Shanghai Ocean University

Lipopolysaccharide-induced TNF-αfactor(LITAF),which participates in innateimmune response and regulates TNF-αtranscription,has been identified and characterized invarious *** a study to screen interacting cellular proteins with grass carp reovirus usingyeast two-hybrid system,a grass carp homologue of LITAF was identified to bind the NS26protein encoded by the S11 genomic fragment of grass carp reovirus(GCRV).In this study,grasscarp LPS-induced TNF-αfactor gene(designated as CiLITAF)was cloned and sequenced fromthe cDNA library constructed for the yeast two-hybrid *** CiLITAF cDNA containedan open reading frame(ORF)of 483 bp encoding a polypeptide of 161 amino acids with anestimated molecular mass of 17.0 *** CIK cells infected with GCRV or treated with poly(I:C),transiently stimulated transcription of CiLITAF mRNA was noticed at 8 h post infection or poly(I:C)*** carp TNF-α(CiTNFα)transcriptional level was also transiently induced to ahigh level following the stimulation of CiLITAF in these in vitro *** vivo analysis furthershowed that,significantly up-regulated transcriptional expression of both CiLITAF and CiTNFαwere detected in the spleen tissue as early as 48 h post challenge with *** study thuscharacterized CiLITAF as an inducible gene responding to viral infection.

关键词： LITAF TNF-α grass carp(Ctenopharyngodon idella) grass carp reovirus Quantitative RT-PCR

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Dynamic localization and the associated translocation mechanism of HMGBs in response to GCRV challenge in CIK cells

引用

Cellular & Molecular Immunology 2015年第3期12卷 342-353页

作者： Youliang Rao Jianguo Su Chunrong Yang Nana Yan Xiaohui Chen Xiaoli Feng College of Animal Science and Technology Northwest A&F University Yangling China

High-mobility group box （HMGB） proteins, a family of chromatin-associated nuclear proteins, play amazingly multifaceted roles in the immune system of mammals. Thus far, little is known about the nucleocytoplasmic distribution of HMGBs in teleosts. The present study systematically investigated the dynamic localization of all six HMGB proteins in Ctenopharyngodon idella kidney （CIK） cells. Under basal conditions, all HMGBs exclusively localized to the nucleus. grass carp reovirus （GCRV）, polyinosinic-polycytidylic （poly（I ： C）） potassium salt and lipopolysaccharide （LPS） challenge evoked the nuclear export of HMGBs to various degrees： GCRV challenge induced the highest nuclear export of CiHMGB2b, and poly（I ~ C） and LPS evoked the highest nucleocytoplasmic shuttling of CiHMGBlb. Overall, the nucleocytoplasmic shuttling of CiHMGB2a and CiHMGB3b was rarely induced by these challenges. Dynamic imaging uncovered that the nucleocytoplasmic GCRV-induced relocation of CiHMGB2b occurred in cells undergoing karyotheca rupture, apoptosis or proliferation. Western blot analyses were used to examine HMGB-EGFP fusion proteins in whole cell lysates, cytosol, nuclear fractions and culture medium. Further investigation demonstrated the nuclear retention of N-terminal HMG-boxes and the nucleocytoplasmic distribution of the C-terminal acidic tails. Comparative analyses of the dynamic relocation of full-length, truncated or chimeric HMGBs confirmed that the intramolecular interaction between HMG-boxes and C-tail domains mediated the nucleocytoplasmic translocation of HMGBs. These results not only provide an overall understanding of the subcellular localization of HMGBs, but also reveal the induction mechanism of the nucleocytoplasmic translocation of HMGBs by GCRV challenge, which lays a foundation for further studies on the interactions among pathogens, HMGBs and pattern recognition receptors in the innate immunity of teleosts.

关键词： grass carp (Ctenopharyngodon idella) grass carp reovirus HMGBs innate immunity subcellular localization

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Characterization of grass carp FosB, Fosl2, JunD transcription factors in response to GCRV infection

引用

Aquaculture and Fisheries 2022年第3期7卷 304-312页

作者： Fei Yu Wanjuan Li Longlong Wang Shunzheng Que Liqun Lu National Pathogen Collection Center for Aquatic Animals Shanghai Ocean UniversityShanghai201306China Institute of Marine Biology College of OceanographyHohai UniversityNanjing210098China Key Laboratory of Agriculture Ministry for Freshwater Aquatic Genetic Resources Shanghai Ocean UniversityShanghai201306China National Experimental Teaching Demonstration Center for Fishery Sciences Shanghai Ocean UniversityShanghai201306China

Activating protein-1(AP-1)composed of Jun and Fos family proteins,which are frequently activated by pathogens to induce cellular inflammation and immune *** this study,by using RNA sequencing(RNA-seq)technology,we found that FosB,Fosl2,and JunD expression was co-induced 12 and 20 h in grass carp(Ctenopharyngodon idella)kidney cell line(CIK cells)after grass carp reovirus(GCRV)***-length cDNA of the three genes were cloned and phylogenetic analysis indicated that the corresponding proteins shared high homology with those in higher *** tissue distribution analysis of the three genes showed they were ubiquitously expressed in various tissues of healthy grass *** infection assay,FosB,Fosl2,and JunD were up-regulated and co-expressed 8 h after GCRV infection in CIK *** interaction and co-expression network analysis revealed that FosB,Fosl2,and JunD showed similar functions and were co-expressed in mammals and *** together,these results indicated that the co-expression of the AP-1 family proteins in grass carp uniformly responds to GCRV *** findings provided insights into the further study of AP-1 transcription factors function during grass carp reovirus infection.

关键词： grass carp reovirus FosB Fosl2 JunD Co-expression Inflammation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

草鱼尾鳍组织二倍体细胞系GCCF-2的建立及其部分生物学特性的分析

引用

水生生物学报 1986年第3期10卷 293-294页

作者：魏彦章,陆仁后,白国栋中国科学院水生生物研究所中国科学院水生生物研究所中国科学院水生生物研究所武汉武汉武汉

我们从1983年9月至1985年1月,将草鱼尾鳍组织细胞在体外培养17个月,传代100次,确立为建立的细胞系(established cell lioe),命名为GCCF-2。1.细胞系的建立无菌获取草鱼尾鳍组织,采用组织块培养法置TC-199培养液中进行初代培养。20代之... 详细信息

我们从1983年9月至1985年1月,将草鱼尾鳍组织细胞在体外培养17个月,传代100次,确立为建立的细胞系(established cell lioe),命名为GCCF-2。1.细胞系的建立无菌获取草鱼尾鳍组织,采用组织块培养法置TC-199培养液中进行初代培养。20代之前培养液中小牛血清含量为20%;20代之后,小牛血清含量降为15%。培养温度为28—30℃。1∶4传代培养,4—5天传代一次。2.细胞形态由于在初代培养及以后的传代培养过程中未加任何选择,GCCF-2为上皮样细胞和成纤维样细胞的混合细胞系。但上皮样细胞占多数。绝大多数细胞为单核,二、三及多核细胞很少。3.细胞生长速度以10～5细胞/ml的浓度接种培养,第二天细胞数即有所增加,第五天达最

关键词： Caudal fin tissue diploid cell line grass carp reovirus

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：