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Neopestalotiopsis eucalypti,a causal agent of grapevine shoot rot in cutting nurseries in China

Journal of Integrative Agriculture 2022年第12期21卷 3684-3691页

作者： MA Xuan-yan JIAO Wei-qi LI Heng ZHANG Wei REN Wei-chao WU Yan ZHANG Zhi-chang LI Bao-hua ZHOU Shan-yue College of Plant Health and Medicine Qingdao Agricultural University/Engineering Research Center for Precision Pest Management for Fruits and Vegetables of QingdaoQingdao 266109P.R.China Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China Institute of Plant and Environment ProtectionBeijing Academy of Agriculture and Forestry SciencesBeijing 100097P.R.China Shandong Zhichang Agricultural Science and Technology Development Co. Ltd.Rizhao 276500P.R.China

grapevine(Vitis vinifera L.)is an economically important fruit crop in the world,and China ranks first in the production of grapes with approximately 15%of the world’s total ***,diseases that cause the death of grapevine shoots pose a severe threat to the production of *** this study,the fungus Neopestalotiopsis eucalypti was identified as a causal pathogen of grapevine shoot rot based on the morphology of conidia and a phylogenetic *** phylogenetic analysis was performed with three isolates based on the combined sequence of internal transcribed spacer(ITS)region of ribosomal DNA,part of the translation elongation factor 1-alpha(Tef)and theβ-tubulin(Tub2)*** three isolates were all identified as *** tests of the three fungal isolates were conducted on grapevines shoots in vitro and in *** results showed that all three fungal isolates caused severe rot lesions on the inoculated grapevine shoots,and *** was re-isolated from the inoculated grapevine ***,*** was confirmed as a causal agent of the grapevine shoot *** is the first report of *** causing grapevine shoot disease in China.

关键词： Neopestalotiopsis eucalypti grapevine shoot rot multigene phylogenetic analysis pathogenicity

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