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Recombinant human granulocyte-colony stimulating factor and differential expression of cerebral cortical proteins in the subacute stage of cerebral ischemia/reperfusion

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Neural Regeneration Research 2011年第19期6卷 1478-1483页

作者： Baohua Liu Jing Dong Lei Lu Ying Sha Lei Song Qun Liu Department of Neurology First Hospital of Jilin University Changchun 130021 Jilin Province China Department of Neurology Harrison Intemational Peace Hospital Hengshui 053000 Hebei Province China

Recombinant human granulocyte-colony stimulating factor （hG-CSF） has been shown to protect the nervous system after brain ischemia. However, the neuroprotective mechanism of hG-CSF remains unclear. The present study established a rat model of cerebral ischemia/reperfusion and subcutaneously injected recombinant hG-CSF after reperfusion for 2 hours. Cerebral cortical protein was extracted following 14 days of reperfusion and subjected to two-dimensional electrophoresis. In brain ischemic rats, 56 different protein spots were screened, including 17 that were upregulated and 17 that were downregulated, compared with the sham-surgery group. Matrix assisted laser desorption ionization/time of flight mass spectrometry was used to determine peptide mass fingerprinting. Following a National Center for Biotechnology Information database search and confirmation with the Swiss-Prot database, 19 spots were identified as known proteins. Following hG-CSF treatment, 35 different protein spots were found, including 16 that were downregulated and 19 that were upregulated. Six were known proteins, including dihydropyrimidinase-associated protein 2, glial fibrillary acidic protein, endomucin, Rho GDP dissociation inhibitor, Rab GDP dissociation inhibitor and guanine-nucleotide-binding protein. Results indicate that hG-CSF is involved in neuroprotection after brain ischemia, possibly by regulating the expression of various neural regeneration-associated proteins at the subacute stage.

关键词： granulocyte-colony stimulating factor brain ischemia cerebral infarction proteome neural regeneration

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Capillary Zone Electrophoresis Investigation of Interactions between granulocyte-colony stimulating factor and Dextran Sulfate/Carrageenan Oligosaccharide

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Chinese Chemical Letters 2005年第5期16卷 647-650页

作者： AiYeLIANG YuGuangDU KeYiWANG BingChengLIN DalianInstituteofChemicalPhysics ChineseAcademyofSciencesDalian116023 InstituteofBiochemistryandCellBiology ShanghaiAcademyofLifeScienceChineseAcademyofSciencesShanghai200031

The interactions between granulocyte-colony stimulating factor (G-CSF) and dextran sulfate / κ-carrageenan oligosaccharide were studied by capillary zone electrophoresis. Dextran sulfate could strongly interact with G-CSF and the complex was detected. The binding constant and stoichiometry were determined to be 1.2×106 (mol/L)-1 and 3:1, respectively. However, the interaction between κ-carrageenan oligosaccharide and G-CSF was not found.

关键词： Capillary zone electrophoresis granulocyte-colony stimulating factor dextran sulfate κ-carrageenan oligosaccharide interaction

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Soluble factors secreted by human Wharton’s jelly mesenchymal stromal/stem cells exhibit therapeutic radioprotection: A mechanistic study with integrating network biology

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World Journal of Stem Cells 2022年第5期14卷 347-361页

作者： Dharmendra Kumar Maurya Mayuri Bandekar Santosh Kumar Sandur Radiation Biology and Health Sciences Division Bhabha Atomic Research CentreMumbai 400085MaharashtraIndia Homi Bhabha National Institute AnushaktinagarMumbai 400094India University of Mumbai KalinaMumbai 400098India

BACKGROUND Human Wharton’s jelly-derived mesenchymal stromal/stem cells(hWJ-MSCs)have gained considerable attention in their applications in cell-based therapy due to several advantages offered by ***,we reported that hWJ-MSCs and their conditioned medium have significant therapeutic radioprotective *** finding raised an obvious question to identify unique features of hWJ-MSCs over other sources of stem cells for a better understanding of its radioprotective *** To understand the radioprotective mechanism of soluble factors secreted by hWJMSCs and identification of their unique *** Propidium iodide staining,endogenous spleen colony-forming assay,and survival study were carried out for radioprotection ***-driven proliferation assay was performed for in vivo lymphocyte *** of RNAseq data was performed to find the unique genes of WJ-MSCs by comparing them with bone marrow mesenchymal stem cells,embryonic stem cells,and human *** enrichment analysis and protein-protein interaction network were used for pathway *** Co-culture of irradiated murine splenic lymphocytes with WJ-MSCs offered significant radioprotection to ***-MSC transplantation increased the homeostasis-driven proliferation of the *** of WJ-MSC conditioned medium with granulocyte-colony stimulating factor antibody abolished therapeutic *** analysis showed that WJ-MSCs share several common genes with bone marrow MSCs and embryonic stem cells and express high levels of unique genes such as interleukin(IL)1-α,IL1-β,IL-6,CXCL3,CXCL5,CXCL8,CXCL2,CCL2,FLT-1,and *** was also observed that WJ-MSCs preferentially modulate several cellular pathways and processes that handle the repair and regeneration of damaged tissues compared to stem cells from other ***-based network analysis showed that most of the radiosensitive tissues have a more com

关键词： Radioprotection Mesenchymal stem cells Wharton’s jelly-derived mesenchymal stromal/stem cells Cytokines granulocyte-colony stimulating factor Network biology

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Dynamic changes in growth factor levels over a 7-day period predict the functional outcomes of traumatic brain injury

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Neural Regeneration Research 2018年第12期13卷 2134-2140页

作者： Shuai Zhou Dong-Pei Yin Yi Wang Ye Tian Zeng-Guang Wang Jian-Ning Zhang Department of Intensive Care Unit Tianjin Medical University General Hospital Tianjin China Tianjin Neurological Institute Tianjin China Key Laboratory of Post-Trauma Neuro-Repair and Regeneration in Central Nervous System Ministry of Education Tianjin China Tianjin Key Laboratory of Injuries Variations and Regeneration of Nervous System Tianjin China Department of Neurosurgery Tianjin Medical University General Hospital Tianjin China

Traumatic brain injury（TBI） can result in poor functional outcomes and death, and overall outcomes are varied. Growth factors, such as angiopoietin-1（Ang-1）, vascular endothelial growth factor（VEGF）, and granulocyte-colony stimulating factor（G-CSF）, play important roles in the neurological functions. This study investigated the relationship between serum growth factor levels and long-term outcomes after TBI. Blood samples from 55 patients were collected at 1, 3 and 7 days after TBI. Blood samples from 39 healthy controls were collected as a control group. Serum Ang-1, G-CSF, and VEGF levels were measured using ELISA. Patients were monitored for 3 months using the Glasgow Outcome Scale-Extended（GOSE）. Patients having a GOSE score of 〉 5 at 3 months were categorized as a good outcome, and patients with a GOSE score of 1-5 were categorized as a bad outcome. Our data demonstrated that TBI patients showed significantly increased growth factor levels within 7 days compared with healthy controls. Serum levels of Ang-1 at 1 and 7 days and G-CSF levels at 7 days were significantly higher in patients with good outcomes than in patients with poor outcomes. VEGF levels at 7 days were remarkably higher in patients with poor outcomes than in patients with good outcomes. Receiver operating characteristic analysis showed that the best cut-off points of serum growth factor levels at 7 days to predict functional outcome were 1,333 pg/mL for VEGF, 447.2 pg/mL for G-CSF, and 90.6 ng/mL for Ang-1. These data suggest that patients with elevated levels of serum Ang-1, G-CSF, and decreased VEGF levels had a better prognosis in the acute phase of TBI（within 7 days）. This study was registered with the Chinese Clinical Trial Registry（registration number： ChiCTR1800018251） on September 7, 2018.

关键词： nerve regeneration traumatic brain injury vascular endothelial growth factor angiopoietin-1 granulocyte-colony stimulating factor outcomes secondary brain injuries blood-brain barrier brain edema acute phase clinical trial neural regeneration

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