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Antidiabetic,antihyperlipidemic and antioxidant potential of methanol extract of Tectona grandis flowers in streptozotocin induced diabetic rats

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Asian Pacific Journal of Tropical Medicine 2011年第8期4卷 624-631页

作者： Subramaniani Ramachandran Aiyalu Rajasekaran KT Manisenthil Kumar Department of Pharmacology KMCH College of PharmacyCoimbatore - 641 048Tamil NaduIndia Department of Pharmaceulical Chemistry KMCH College of PharmacyCoimbatore - 641 048Tamil NaduIndia

Objective:To investigate antidiabetic,antihyperlipidemic and antioxidant activity of methanol extract of Tectona grandis(T.grandis) flowers(METGF) in streplozotocin(STZ) induced diabetic rats to supports its traditional use.Methods:Acute toxicity study of METGF was carried out in rat to determine its dose for the antidiabetic study.Oral glucose tolerance test(OGTT) was performed to evaluate METGF effect on elevated blood glucose level.Diabetes was induced in rats by administration of STZ(60 mg/kg,ip.) and it was confirmed 72 h after induction.METGF was orally given to the diabetic rats up to 28 days and blood glucose level were estimated each week.On 28 day of the experiment,diabetic rats were sacrificed after the blood collection for the biochemical parameters analysis and liver,kidney was collected to determine antioxidants levels.Results: In acute toxicity,METGF did not show toxicity and death up to a dose 2 000 mg/kg in rats. Administration of METGF 100 and 200 mg/kg significantly(P<0.001) reduced blood glucose levels in OGTT and STZ-induced diabetic rats.Both doses of METGF treatment significantly(P<0.001, P<0.01 and P<0.05) increased body weight,serum insulin,haemoglobin(Hb) and total protein levels in diabetic rats.Also,MEGTF treatment reduced elevated glycosylated haemoglobin(HbA1c) and other biochemical parameters levels significantly(P<0.001) in diabetic rats.Altered lipid profiles and antioxidants levels were reversed to near normal in diabetic rats treated with METGF. Conclusions:These results concluded that METGF possesses antidiabetic,antihyperglycemic and antioxidant activity which supports its traditional use.

关键词： Diabetes Streptozotocin Tectona grandis Lipid profile Antioxidant

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Molecular phylogeny and ultrastructure of a new population of Urostyla grandis(Ciliophora, Hypotrichida) from Harbin, China

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Journal of Oceanology and Limnology 2019年第1期37卷 256-265页

作者： CAI Xinglong CHEN Ying ZHAO Xiaolu GAO Jing Saleh A. AL-FARRAJ YU Lijie PAN Xuming QIU Zijian Laboratory of Protozoology Harbin Normal University Institute of Evolution & Marine Biodiversity Ocean University of China Zoology Department College of Science King Saud University

A new population of Urostyla grandis, collected from a freshwater pond of northeastern China was investigated by living observation, silver impregnation, and molecular phylogeny based on small subunit ribosomal rRNA(SSU rRNA). Besides, its ultrastructural characteristics were investigated carefully by scanning electron microscopy(SEM) and transmission electron microscopy(TEM) methods.Complementary descriptions of some inner ultrastructures were provided and some new organelles were also found. Especially, the oral inner microtubule system was illustrated by SEM and TEM for the ?rst time and a new type extrusome was reported. These results may supply complement taxonomic data and ingestion mechanism on ciliates in genus Urostyla.

关键词： Urostyla grandis phylogeny ultrastructure new population

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Evaluation of early growth performance of 41 clones of teak(Tectona grandis Linn. f.) at four microsites in Purwakarta,Indonesia

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Journal of Forestry Research 2020年第3期31卷 901-907页

作者： Asep Mulyadiana Trikoesoemaningtyas Iskandar ZSiregar Tropical Silviculture Program Graduate SchoolBogor Agricultural University(IPB)Kampus IPB DramagaBogor 16680Indonesia Department of Agronomy and Horticulture Faculty of AgricultureBogor Agricultural University(IPB)Kampus IPB DramagaBogor 16680Indonesia Department of Silviculture Faculty of ForestryBogor Agricultural University(IPB)Kampus IPB DramagaBogor 16680Indonesia

Teak(Tectona grandis Linn. f.), one of the mostvalued tree species in the world, is slow-growing with a long period until it can be harvested;therefore, ensuring that only high-quality seedlings or clones are selected for planting is critical. The main objective was to determine performance and repeatability of selected clones in terms of growth and survival rates in different micro-environments. A 2-year clonal trial using 41 clones and a local seedling of teak as a control were grown at 4 microsites differing in spacing, soil fertility and alley crops to assess tree height, diameter and survival rate that was evaluated in Purwakarta, West Java, Indonesia using a randomized complete block design with four replicates of each clone at each microsite. Teak growth was influenced by clone(p < 0.01), microsite(p < 0.05) and clone 9 micrositeinteraction(p<0.01). The interaction clone 9 microsite resulted in several potential superior clones that differed in terms of growth rates at each microsite. On the basis of diameter only, superior clones(nos. 14, 18, 24, 30 and 37)were identified. Repeatability estimated was Rc2= 0.84 for diameter and Rc2= 0.77 for height. Growth performance of teak trees varied among microsites. Repeatability values for diameter and height characters were high. The effect of variable growth on each clone was influenced by genetic factors, environmental factors and the interaction of genetics 9 environment. Microsite significantly affected growth of teak clones. Clone 9 microsite interaction significantly affected growth of clones and led to the growth of superior clones at each microsite.

关键词： Clonal trial Clones Repeatability Survival rate Tectona grandis

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The potential application of the traditional Chinese herb Exocarpium Citri grandis in the prevention and treatment of COVID-19

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Traditional Medicine Research 2020年第3期5卷 160-166页

作者： Wei-Wei Su Yong-Gang Wang Pei-Bo Li Hao Wu Xuan Zeng Rui Shi Yu-Ying Zheng Pan-Lin Li Wei Peng Guangdong Engineering&Technology Research Center for Quality and Efficacy Reevaluation of Post-Market Traditional Chinese Medicine Guangdong Provincial Key Laboratory of Plant ResourcesSchool of Life SciencesSun Yat-sen UniversityGuangzhou 510275China

Huajuhong(Exocarpium Citri grandis,ECG)is a traditional Chinese herbal medicine and has been used for the treatment of respiratory diseases for hundreds of years.Recently,ECG has been listed in a traditional Chinese medicine formula in the Guidelines for the Diagnosis and Treatment of Coronavirus Disease 2019(sixth edition)in China.To date,the effect and mechanism of ECG against respiratory diseases have not been systematically reviewed.In this paper,the researchers summarized the effects of ECG and its pharmacologically active compound naringin in functioning as an antitussive and expectorant,improving lung function,alleviating acute lung injury,attenuating pulmonary fibrosis,and enhancing antiviral immune response,so as to provide a reference for its clinical application in the prevention and treatment of multiple respiratory diseases,including coronavirus disease 2019.

关键词： Huajuhong Exocarpium Citri grandis Naringin coronavirus disease 2019 Clinical application

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Construction of a Full-Length cDNA Library of Solen grandis Dunker and Identification of Defense- and Immune-Related Genes

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Journal of Ocean University of China 2014年第1期13卷 169-173页

作者： SUN Guohua LIU Xiangquan REN Lihua YANG Jianmin WEI Xiumei YANG Jialong Shandong Provincial Key Laboratory of Restoration for Marine Ecology Shandong Marine Fisheries Research Institute Yantai Institute of Coastal Zone Research Chinese Academy of Sciences

The basic genetic characteristics, important functional genes, and entire transcriptome of Solen grandis Dunker were investigated by constructing a full-length cDNA library with the ‘switching mechanism at the 5'-end of the RNA transcript'(SMART) technique. Total RNA was isolated from the immune-relevant tissues, gills and hemocytes, using the Trizol reagent, and cDNA fragments were digested with Sfi I before being ligated to the pBluescript II SK* vector. The cDNA library had a titer of 1048 cfu μL-1 and a storage capacity of 1.05×106 cfu. Approximately 98% of the clones in the library were recombinants, and the fragment lengths of insert cDNA ranged from 0.8 kb to 3.0 kb. A total of 2038 expressed sequence tags were successfully sequenced and clustered into 965 unigenes. BLASTN analysis showed that 240 sequences were highly similar to the known genes(E-value 80%), accounting for 25% of the total unigenes. According to the Gene Ontology, these unigenes were related to several biological processes, including cell structure, signal transport, protein synthesis, transcription, energy metabolism, and immunity. Fifteen of the identified sequences were related to defense and immunity. The full-length cDNA sequence of HSC70 was obtained. The cDNA library of S. grandis provided a useful resource for future researches of functional genomics related to stress tolerance, immunity, and other physiological activities.

关键词： Solen grandis cDNA library EST sequence defense and immune functional gene

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Effects of Different Fertilization Treatments on Soil, Leaf Nutrient and Fruit Quality of Citrus grandis var. longanyou

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World Journal of Engineering and Technology 2017年第2期5卷 1-14页

作者： Rongfei Li Yaodong Chang Tao Hu Xueyou Jiang Guolu Liang Zhiming Lu Youwen Yi Qigao Guo College of Horticulture and Landscape Architecture Southwest University Beibei Chongqing 400716 China Guang’an District Agricultural Bureau Guang'an Sichuan 638500 China Guang’an Science and Technology Development and Training Center Guang'an Sichuan 638500 China

The five-year-old “Longanyou” trees were used as the experimental material to study the effects of different fertilization treatments. The nutrient contents in soil and leaves, fruit yield and quality were determined, and then the correlations were analyzed. The results showed that: 1) The soil nutrient contents of 0 - 20 cm depth were more than the 20 - 40 cm, and the trends of nutrient contents of the 0 - 20 cm soil layers were as follows: treatment 2 (T2) > treatment 3 (T3) > treatment 4 (T4) > treatment 1(T1) > control (CK). However, the 20 - 40 cm depth had not significant difference between different treatments, but T2, T4 and T3 were higher than T1 and CK. It indicated that the soil effective nutrient content increased in T2 and T3. 2) Compared with the control, the content of K and B elements was improved obviously in leaves with the increase of organic manure application. The contents of P (1.60 g·kg-1), B (26.00 mg·kg-1) and Mg (1.18 g·kg-1) were the highest, and other nutrients contents were also higher, indicating that T2 could effectively improve the leaves’ nutrient contents. 3) The fruit yield per plant was the highest in T2 (95.40 kg plant-1), and the single fruit weight, total sugar, sugar and acid ratio, vitamin C were also the highest, but titratable acid was lower. It indicated that T2 effectively improved fruit yield and quality. 4) There were positive correlations between multiple factors of soil nutrients and the quality index, such as fruit peel thickness, total sugar, solid acid ratio, sugar and acid ratio, Vc content and single yield etc. There was significant correlation between K, B, Zn, Fe contents and fruit yield and quality index, and the contents of B, Zn and Fe in leaves were significantly correlated with soil nutrient, indicating that the contents of K, B, Zn, Fe in soil and leaf were closely related to fruit yield and quality. In sum, the T2 was the best fertilization scheme for orchard management practice of “Longanyou”.

关键词： Citrus grandis var. longanyou Fertilization Soil Nutrient Leaf Nutrient Fruit Quality

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Rejuvenation and Adventitious Rooting in Coppice-Shoot Cuttings of <i>Tectona grandis</i>as Affected by Stock-Plant Etiolation

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American Journal of Plant Sciences 2011年第3期2卷 370-374页

作者： Azamal Husen 不详

The effect of stock-plant etiolation on adventitious rooting of single-node leafy cuttings (SNCs) made from coppice shoots from different age groups of donor plants were investigated for teak (Tectona grandis Linn. f.). When donor plants age were 1-, 2-, 3-, 4-, and 5-year-old, they were coppiced and maintained in the dark for etiolation, while a parallel set was kept in normal light in an open environment. After 20 days, coppice shoots were made into SNCs which were cultured under intermittent mist for rooting. Stock-plant etiolation significantly increased percent rooting, shoot and root length, and number of roots per SNC, but callusing at the base of SNCs decreased. Etiolated SNCs have shown 71.7% rooting, whereas for controls (non-etiolated), the response was 41.7%. The effect of aging on callusing, rooting and sprouting of etiolated and non-etiolated coppice shoots cuttings varied widely. Aging of donor plants decreased rooting and sprouting capability in SNCs and increased callusing at the base of etiolated cuttings. The results showed that stock-plant etiolation in 1-, 2-, 3-, 4-, and 5-year-old donor plants hastened rooting potential by rejuvenation of coppice shoots.

关键词： Adventitious Rooting Cuttings Etiolation Maturity Tectona grandis

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Identification and Control of HLB Disease in Citrus grandis

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Asian Agricultural Research 2019年第3期11卷 78-82页

作者： Derong YANG Zhiwei ZENG Long ZHOU Jinping LI Chen XU Lingxiang ZHOU Fang WANG Yunnan Yuntianhua Co. Ltd. Yunnan Chemical Research Institute Yunnan Agricultural University

HLB disease has been endangering citrus production,and it is an important task in Citrus grandis production to identify and control HLB disease. Diaphorina citri and Trioza erytreae are the main vectors for the spreading of HLB disease. Scientific and proper release of predatory natural enemies such as ladybugs,combined with chemical control can effectively control psyllid. For suspected HLB disease strains,a simple " HLB disease detection reagent" can be used for detection. This method is simple,cheap and accurate,and it is an economical and feasible identification method for ordinary growers.

关键词： Citrus grandis HLB disease Identification Control measures

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High-yielding Fertilization Technology for Citrus grandis

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Asian Agricultural Research 2019年第4期11卷 73-76页

作者： Derong YANG Ming SHEN Zhiwei ZENG Long ZHOU Yunnan Yuntianhua Co. Ltd. Yunnan Chemical Research Institute Yunnan Hengguan Taida Agricultural Development Co. Ltd. Yunnan Agricultural University

As of April 2017,the planting area of Citrus grandis in Ruili City was 594. 3 ha. Among it,the area of fruiting trees was 267. 2 ha,and they were mainly owned by small growers. In view of the serious fragmentation of pomelos,the large investment in fertilizers,and the unsatisfactory production efficiency of C. grandis in Ruili,the fertilization technology for C. grandis was discussed. The problems in the fertilization process,as well as the principles and modes of fertilization were analyzed briefly. The fertilization methods for saplings and fruiting trees were introduced,in order to promote the improvement of the fertilization and management level of local pomelo growers.

关键词： Citrus grandis Cultivation technique Fertilization

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grandis电控节气门系统的原理和检修

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汽车电器 2009年第3期 32-36页

作者：何金戈海南大学机电学院汽车系海南儋州571737

详细介绍三菱grandis线控电子节气门系统的组成、构造和工作原理,以及检修和自适应方法。并用维修实例加以说明。

关键词： grandis 电子节气门工作原理检修

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