检索结果-南通市图书馆

Increased oxidative damage of sperm and seminal plasma in men with idiopathic infertility is higher in patients with glutathione s-transferase mu-1 null genotype

引用

Asian Journal of Andrology 2007年第1期9卷 108-115页

作者： Birsen Aydemir Ilhan Onaran Ali R. Kiziler Bulent Alici Mehmet C. Akyolcu Department of Biophysics Cerrahpasa Medical Faculty lstanbul University lstanbul 34098 Turkey Department of Medical Biology Cerrahpasa Medical Faculty lstanbul University lstanbul 34098 Turkey Department of Urology Cerrahpasa Medical Faculty lstanbul University lstanbul 34098 Turkey

Aim： To examine whether a relationship exists between glutathione s-transferase mu-1 （GsTM1） gene polymorphism and the susceptibility of sperm and seminal plasma from patients with idiopathic infertility to oxidative stress. Methods： Fifty-two men with idiopathic infertility and 60 healthy fertile men were recruited to this study. GsTM1 gene polymorphism was determined by polymerase chain reaction （PCR） and both the infertile and control individuals were divided into GsTM1 null and GsTM1 positive groups according to their GsTM1 gene structure. We compared reactive oxygen species （ROs） generation, malondialdehyde （MDA）, protein carbonyls and glutathione （GsH） concentrations, and glutathione s-transferase （GsT） activity in seminal plasma and spermatozoa from infertile patients and controls with respect to GsTM1 genotype. Results： significantly higher levels of oxidative stress and damage markers were found in idiopathic infertile men with the GsTM1 null genotype compared with those with the GsTM1 positive genotype. There was no significant difference in genotype distribution for theGsTM1 variant between the idiopathic infertile subjects and fertile subjects. Patients with the GsTM1 null genotype also had lower sperm concentrations than those with GsTM1 positive genotype. Conclusion： Our results suggest that the susceptibility of sperm and seminal plasma to oxidative stress is significantly greater in idiopathic infertile men with the GsTM1 null genotype compared with those possessing the gene. Therefore, in patients with idiopathic infertility, GsTM1 polymorphism might be an important source of variation in susceptibility of spermatozoa to oxidative damage.

关键词： idiopathic infertility glutathione s-transferase mu-1 GsTM1 polymorphism semen sperm oxidative stress

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：