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Resveratrol prevents inner retinal dysfunctions and regulates glutamate transporters and glutamine synthetase expressions in diabetic rats retinas

Resveratrol prevents inner retinal dysfunctions and regulate...

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第七届全国中西医结合营养学术会议

作者：曾凯宏杨娜余雪梅宋怡邓波吴晓娜周雪黄璐娇杨咏涛四川省人民医院营养科四川省人民医院实验动物所

*** purpose of the present study was to investigate the effects of resveratrol(RSV)on retinal function and on glutamate transporters(GLAST)and glutamine synthetase(GS)expressions in diabetic rats retinas..***-Dawley rats were divided into normal control(CON),diabetic(DM),normal rats treated with 5 mg/kg/d RSV(CON+L-RSV)and 10 mg/kg/d RSV(CON+H-RSV),and diabetic rats treated with 5 mg/kg/d RSV(DM+L-RSV)and 10 mg/kg/d RSV(DM+H-RSV).The electroretinogram technology was used to evaluate retinal functions of rats from CON,DM,CON+L-RSV,CON+H-RSV,DM+L-RSV and DM+H-RSV groups at 1 month,3,5,and 7 *** Realtime Polymerase Chain Reaction(qRT-PCR)and ELISA analysises were used to research the GLAST and GS expressions in rats ***.7 months RSV administrations(5 and 10 mg/kg/d)significantly alleviated hyperglycemia and weight loss in diabetic rats.1 month,3,5,and 7 months RSV administrations also significantly attenuated diabetes-induced decrease in amplitude of a-wave in rod response,decreases in amplitude of a-,and b-wave in cone and rod response and decrease in amplitude of OP2 in oscillatory *** addition,7 months RSV treatments significantly inhibited diabetesinduced delay in OP2 implicit times in scotopic 3.0 OPS ***,the down-regulated mRNA and protein expressions of GLAST and GS in diabetic rats retinas were prevented by RSV administrations(5and 10 mg/kg/d)at 1 month,3,5,and 7 *** beneficial antidiabetic results suggest that RSV may be considered as a therapeutic option to prevent from diabetic retinopathy.

关键词： resveratrol inner retinal dysfunctions glutamate transporters glutamine synthetase diabetes diabetic retinopathy

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Resveratrol prevents inner retinal dysfunctions and regulates glutamate transporters and glutamine synthetase expressions in diabetic rats retinas

Resveratrol prevents inner retinal dysfunctions and regulate...

引用

第六届全国中西医结合营养学术会议

作者： Kaihong Zeng Na Yang Duozi Wang Suping Li Jian Ming Jing Wang Xuemei Yu Yi Song Xue Zhou Yongtao Yang Department of Clinical Nutrition Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital Institute of Laboratory Animals Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital Department of Neurology Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital Department of Food Science Southwest University

*** purpose of the present study was to investigate the effects of resveratrol(RSV) on retinal function and on glutamate transporters(GLAST) and glutamine synthetase(GS) expressions in diabetic rats ***-Dawley rats were divided into normal control(CON),diabetic(DM),normal rats treated with 5 mg/kg/d RSV(CON+L-RSV) and 10 mg/kg/d RSV(CON+H-RSV),and diabetic rats treated with 5 mg/kg/d RSV(DM+L-RSV) and 10 mg/kg/d RSV(DM+H-RSV).The electroretinogram technology was used to evaluate retinal functions of rats from CON,DM,CON+L-RSV,CON+H-RSV,DM+L-RSV and DM+H-RSV groups at 1 month,3,5,and 7 *** Real-time Polymerase Chain Reaction(qRT-PCR) and ELISA analysises were used to research the GLAST and GS expressions in rats ***.7 months RSV administrations(5 and 10 mg/kg/d) significantly alleviated hyperglycemia and weight loss in diabetic rats.1 month,3,5,and 7 months RSV administrations also significantly attenuated diabetes-induced decrease in amplitude of a-wave in rod response,decreases in amplitude of a-,and b-wave in cone and rod response and decrease in amplitude of OP2 in oscillatory *** addition,7 months RSV treatments significantly inhibited diabetes-induced delay in OP2 implicit times in scotopic 3.0 OPS ***,the down-regulated mRNA and protein expressions of GLAST and GS in diabetic rats retinas were prevented by RSV administrations(5 and 10 mg/kg/d) at 1month,3,5,and 7 *** beneficial antidiabetic results suggest that RSV may be considered as a therapeutic option to prevent from diabetic retinopathy.

关键词： resveratrol inner retinal dysfunctions glutamate transporters glutamine synthetase diabetes diabetic retinopathy

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glutamate in cancers:from metabolism to signaling

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The Journal of Biomedical Research 2020年第4期34卷 260-270页

作者： Haowei Yi Geoff Talmon Jing Wang Department of Genetics Cell Biology and AnatomyUniversity of Nebraska Medical CenterOmahaNE 68198USA. Department of Pathology and Microbiology University of Nebraska Medical CenterOmahaNE 68198USA. Eppley Institute for Research in Cancer and Allied Diseases University of Nebraska Medical CenterOmahaNE 68198USA.

Glutamine and glutamate are major bioenergy substrates for normal and cancer cell *** cells need more biofuel than normal tissues for energy supply,anti-oxidation activity and biomass *** related to metabolic chains in many cancers are somehow mutated,which makes cancer cells more glutamate ***,glutamate is an excitatory neurotransmitter for conducting signals through binding with different types of receptors in central neuron ***,increasing evidences have shown involvement of glutamate signaling,guided through their receptors,in human *** of glutamate transporters,such as excitatory amino acid transporter and cystine/glutamate antiporter system,also generates excessive extracellular glutamate,which in turn,activates glutamate receptors on cancer cells and results in malignant *** features make glutamate an attractive target for anti-cancer drug development with some glutamate targeted but blood brain barrier impermeable anti-psychosis drugs under *** discussed the relevant progressions and drawbacks in this field herein.

关键词： glutamate cancers metabolism glutamate receptors glutamate transporters

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Dietary Omega-3 Fatty Acids Deficiency Affects the glutamatergic Transport System in Rat Retina:Modulatory Effects after High Intraocular Pressure

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Food and Nutrition Sciences 2013年第9期4卷 195-201页

作者： Letícia V.Siqueira Eduardo P.Rico Mário C.Bulla Luciano Bellini Luiz C.L.Silveira Lúcia Vinadé Diogo O.Souza Júlia D.Moreira Biochemistry Department Basic Sciences of Health InstituteFederal University of Rio Grande do SulPorto AlegreBrazil Phy-siology Department Biological Science CenterFederal University of ParáBelémBrazil Master Graduation Program in Biological Sciences Campus Sao GabrielFederal University of Pampa—UNIPAMPASao GabrielBrazil Department of Nutrition Health Sciences CentreFederal University of Santa CatarinaCampus Universitário TrindadeFlorianópolisBrazil

glutamate excitotoxicity has been postulated as a putative mechanism involved in the physiopathology of glaucoma, a disease that can cause retinal cell damage. Thus, the modulation of glutamatergic parameters is a putative therapeutic target to prevent excitotoxic retinal injury. Here, we investigated the effect of dietary omega-3 fatty acids (w3) in the retinal glutamate transport system in basal and ischemic conditions. Female Wistar rats were divided into two groups: w3 diet (w3 group) and w3 deficient-diet (D group). Their pups, at 60 days old, were used for the experiments. Retinal ischemia, a mechanism involved in the physiopathology of glaucoma, was induced by high intraocular pressure (HIOP, 140 180 mmHg for 45 min) to impair retinal blood flow. Analyses were performed 7 days after ischemia. The D group showed a decreased glutamate uptake in basal conditions and after HIOP when compared to the w3 group. After HIOP, there was a decrease in glutamate uptake in the D group that was not observed in the w3 group (p glutamate transporters, the w3 group presented higher levels of GLT-1 compared to the D group in basal and ischemic conditions. After HIOP, EAAC1 was increased in both groups, while GLT-1 increased only in the D group, compared to basal levels. GLAST and EAAT5 presented no alterations. The modulation of the glutamatergic system by dietary w3 fatty acids points to a potential mechanism by which w3 PUFAs exert beneficial effects in the retina.

关键词： N-3 PUFAs Retina Ischemia HIOP Glaucoma glutamate transporters

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