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Fecal microbiota transplantation:whole grain highland barley improves glucose metabolism by changing gut microbiota

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Food Science and Human Wellness 2024年第4期13卷 2014-2024页

作者： Xin Ren Fulong Zhang Min Zhang Yuan Fang Zenglong Chen Meili Huan Beijing Advanced Innovation Center for Food Nutrition and Human Health Beijing Engineering and Technology Research Center of Food AdditivesNational Grain Industry Highland Barley Deep Processing Technology Innovation CenterBeijing Technology and Business UniversityBeijing 100048China State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of ZoologyChinese Academy of SciencesBeijing 100101China COFCO Nutrition and Health Research Institute Beijing 102209China

Highland barley(HB)is a high-altitude cereal with rich nutritional components and potential health benefits.To clarify its hypoglycemic effect and mechanism,we investigated the effect of whole grain HB and fecal microbiota transplantation(FMT)on glucose metabolism and gut microbiota in high-fat diet and streptozotocin(HFD/STZ)-induced diabetic mice.The results showed that HB(40%)significantly decreased fasting blood glucose and the area under the glucose tolerance curve,significantly increased insulin secretion and improved insulin resistance in HFD/STZ-induced diabetic mice(Pglucose metabolism and alleviated the inflammatory state in HFD/STZ-induced diabetic mice.Collectively,our study confirmed the bridge role of gut microbiota in improving glucose metabolism of whole grain HB,which could promote the development of precision nutrition.

关键词： Highland barley Diabetes glucose metabolism Gut microbiota Fecal bacteria transplantation

Voluntary wheel running ameliorated the deleterious effects of high-fat diet on glucose metabolism,gut microbiota and microbial-associated metabolites

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Food Science and Human Wellness 2024年第3期13卷 1672-1684页

作者： Ling Zhang Wenyu Zou Yongyan Hu Honghua Wu Ying Gao Junqing Zhang Jia Zheng Department of Endocrinology Peking University First HospitalBeijing 100034China Laboratory Animal Facility Peking University First HospitalBeijing 100034China

Exercise training is critical for the early prevention and treatment of obesity and diabetes mellitus.However,the mechanism with gut microbiota and fecal metabolites underlying the effects of voluntary wheel running on high-fat diet induced abnormal glucose metabolism has not been fully elaborated.C57BL/6 male mice were randomly assigned to 4 groups according to diets(fed with normal chow diet or high-fat diet)and running paradigm(housed in static cage or with voluntary running wheel).An integrative 16S rDNA sequencing and metabolites profiling was synchronously performed to characterize the effects of voluntary wheel running on gut microbiota and metabolites.It showed that voluntary wheel running prevented the detrimental effects of high-fat feeding on glucose metabolism 16S rDNA sequencing showed remarkable changes in Rikenella and Marvinbryantia genera.Metabolic profiling indicated multiple altered metabolites,which were enriched in secondary bile acid biosynthesis signaling.In conclusion,our study indicated that voluntary wheel running significantly improved glucose metabolism and counteracted the deleterious effects of high-fat feeding on body weight and glucose intolerance.We further found that voluntary wheel running could integratively program gut microbiota composition and fecal metabolites changes,and may regulate muricholic acid metabolism and secondary bile acid biosynthesis in high-fat fed mice.

关键词： High-fat diet Voluntary wheel running Gut microbiota Metabolomics glucose metabolism

Role of glucose metabolism and signaling pathways at different stages of ovarian folliculogenesis

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生殖与发育医学(英文) 2024年第2期8卷 111-120页

作者： Mo Dan Zeng Zhong-Hong Sui Xin Li Rong Yang Yi-Hua Guangxi Reproductive Medical Center the First Affiliated Hospital of Guangxi Medical University Nanning China Guangxi Key Laboratory of Immunology and metabolism for Liver Diseases Nanning China Key Laboratory of Early Prevention and Treatment for Regional High Frequency Tumor (Guangxi Medical University) Ministry of Education Nanning China Center for Reproductive Medicine Department of Obstetrics and Gynecology Peking University Third Hospital Beijing China Key Laboratory of Assisted Reproduction (Peking University) Ministry of Education Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology National Clinical Research Center for Obstetrics and Gynecology (Peking University Third Hospital) Beijing China

The female ovaries are critical for follicle growth and development in the process known as "folliculogenesis" . This complex process is regulated by various factors, among which the microenvironment around follicles appears to be crucial. According to previous studies, folliculogenesis is an energy-demanding process. In fact, well-balanced follicular energy metabolism is associated with ovarian function and female fertility. Consequently, glucose metabolism has been widely described as the main source of energy during folliculogenesis. Generally, the follicular glucose metabolism profiles change dynamically during follicular development. Metabolic abnormalities during folliculogenesis are associated with aging, primary ovarian insufficiency, and polycystic ovary syndrome, thereby leading to subfertility and infertility in females. The signaling pathways in follicles appear to form a link between glucose metabolism and folliculogenesis. Therefore, a better understanding of glucose metabolism dynamics at different stages of folliculogenesis and the associated signaling pathways will provide potential implications for follicle developmental competence. This review aimed to describe variations in glucose metabolism at different stages of folliculogenesis, provide new insights into glucose metabolic disorder-related diseases, and specifically discuss two major signaling pathways that regulate glucose metabolism during folliculogenesis: phosphatidylinositol 3-kinase, protein kinase B (PI3K-PKB/AKT), and AMP-activated protein kinase (AMPK) signaling pathways.

关键词： Folliculogenesis glucose metabolism Glycolysis Oxidative phosphorylation Signaling pathway

Glycolysis and gluconeogenesis are involved of glucose metabolism adaptation during fasting and re-feeding in black carp(Mylopharyngodon piceus)

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Aquaculture and Fisheries 2024年第2期9卷 226-233页

作者： Yafan Dai Yubang Shen Jiamin Guo Hao Yang Feng Chen Wei Zhang Wenhui Wu Xiaoyan Xu Jiale Li Key Laboratory of Freshwater Aquatic Genetic Resources Ministry of Agriculture and Rural AffairsShanghai Ocean UniversityShanghai201306China Shanghai Engineering Research Centre of Aquaculture Shanghai Ocean UniversityShanghai201306China

Both in nature and in aquaculture,fish may experience periods of food scarcity or hunger.The metabolic regulation of fish when nutritional state changes is a complex process that involves many factors.To study glucose metabolism adaptability during fasting and re-feeding in the black carp(Mylopharyngodon piceus),we measured changes in some biochemical indicators related to glucose metabolism.Five fish were sampled on days 0,1,3,5,and 10 of fasting(F,S1,S3,S5,and S10,respectively)and days 1,3,and 5 of re-feeding(RF1,RF3,and RF5,respectively).The serum glucose concentration decreased significantly at S1,reached the lowest point at S10,and increased significantly at RF1(Pglucose transporter 2(GLUT2)decreased significantly at S1 and increased significantly at RF1 and RF5,respectively(Pglucose-6-phosphatase(G6PC)increased significantly at S1 and decreased significantly at RF1(Pglucose homeostasis du

关键词： Mylopharyngodon piceus Fasting glucose metabolism Glycolysis Gluconeogenesis

Effects of exercise training on glucose metabolism indicators and inflammatory markers in obese children and adolescents:A metaanalysis

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World Journal of Diabetes 2024年第6期15卷 1353-1366页

作者： Le-Yang Li Song-Mei Li Bo-Xian Pang Jun-Ping Wei Qiu-Hong Wang Department of Endocrinology Guang’anmen HospitalChina Academy of Chinese Medical SciencesBeijing 100053China Graduate School Beijing University of Chinese MedicineBeijing 100029China

BACKGROUND Obesity in children and adolescents is a serious problem,and the efficacy of exercise therapy for these patients is controversial.AIM To assess the efficacy of exercise training on overweight and obese children based on glucose metabolism indicators and inflammatory markers.METHODS The PubMed,Web of Science,and Embase databases were searched for randomized controlled trials related to exercise training and obese children until October 2023.The meta-analysis was conducted using RevMan 5.3 software to evaluate the efficacy of exercise therapy on glucose metabolism indicators and inflammatory markers in obese children.RESULTS In total,1010 patients from 28 studies were included.Exercise therapy reduced the levels of fasting blood glucose(FBG)[standardized mean difference(SMD):-0.78;95%confidence interval(CI):-1.24 to-0.32,P=0.0008],fasting insulin(FINS)(SMD:-1.55;95%CI:-2.12 to-0.98,Pglucose metabolism by reducing levels of FBG,FINS,HOMA-IR,as well as improves inflammatory status by reducing levels of IL-6,CRP,leptin,and increasing levels of adiponectin in overweight and obese children.There was no statistically significant effect between exercise training and levels of TNF-α.Additional long-term trials should be conducted to explore this therapeutic perspective and confirm these results.

关键词： Exercise training Obesity Children and adolescents glucose metabolism Inflammatory markers Meta-analysis

Dysfunctional gene splicing in glucose metabolism may contribute to Alzheimer’s disease

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Chinese Medical Journal 2023年第6期136卷 666-675页

作者： Shengfeng Deng Peng Yi Mingliang Xu Qian Yi Jianguo Feng Laboratory of Anesthesiology Department of Anesthesiologythe Affiliated Hospital of Southwest Medical UniversityLuzhouSichuan 646000China Department of Physiology School of Basic Medical SciencesSouthwest Medical UniversityLuzhouSichuan 646000China Affiliated Xinhui Hospital Southern Medical University(People’s Hospital of Xinhui District)JiangmenGuangdong 529100China

The glucose metabolism is crucial for sustained brain activity as it provides energy and is a carbon source for multiple biomacromolecules;glucose metabolism decreases dramatically in Alzheimer’s disease(AD)and may be a fundamental cause for its development.Recent studies reveal that the alternative splicing events of certain genes effectively regulate several processes in glucose metabolism including insulin receptor,insulin-degrading enzyme,pyruvate kinase M,receptor for advanced glycation endproducts,and others,thereby,influencing glucose uptake,glycolysis,and advanced glycation end-products-mediated signaling pathways.Indeed,the discovery of aberrant alternative splicing that changes the proteomic diversity and protein activity in glucose metabolism has been pivotal in our understanding of AD development.In this review,we summarize the alternative splicing events of the glucose metabolism-related genes in AD pathology and highlight the crucial regulatory roles of splicing factors in the alternative splicing process.We also discuss the emerging therapeutic approaches for targeting splicing factors for AD treatment.

关键词： Alzheimer’s disease Alternative splicing glucose metabolism Splicing factors

METTL5 stabilizes c-Myc by facilitating USP5 translation to reprogram glucose metabolism and promote hepatocellular carcinoma progression

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Cancer Communications 2023年第3期43卷 338-364页

作者： Peng Xia Hao Zhang Haofeng Lu Kequan Xu Xiang Jiang Yuke Jiang Xiangdong Gongye Zhang Chen Jie Liu Xi Chen Weijie Ma Zhonglin Zhang Yufeng Yuan Department of Hepatobiliary&Pancreatic Surgery Zhongnan Hospital ofWuhan UniversityWuhanHubeiP.R.China Clinical Medicine Research Center for Minimally Invasive Procedure of Hepatobiliary&Pancreatic Diseases of Hubei Province WuhanHubeiP.R.China Department of Hepatobiliary Surgery The First Affiliated Hospital of Yangtze UniversityJingzhouHubeiP.R.China Department of Clinical and Translational Research Center Shenzhen People’s Hospital(The Second Clinical Medical CollegeJinan UniversityThe First Affiliated HospitalSouthern University of Science and Technology)ShenzhenGuangdongP.R.China

Background:Hepatocellular carcinoma(HCC)is one of the most prevalent cancers in the world,with a high likelihood of metastasis and a dismal prognosis.The reprogramming of glucosemetabolism is critical in the development ofHCC.TheWarburg effect has recently been confirmed to occur in a variety of cancers,including HCC.However,little is known about the molecular biological mechanisms underlying the Warburg effect in HCC cells.In this study,we sought to better understand how methyltransferase 5,N6-adenosine(METTL5)controls the development of HCC and theWarburg effect.Methods:In the current study,quantitative real-time polymerase chain reaction and Western blotting were used to detect the expression of METTL5 in HCC tissues and cell lines.Several different cell models and animal models were established to determine the role of METTL5 in glucose metabolism reprogramming and the underlying molecularmechanism of HCC.Glutathione-S-transferase pulldown,coimmunoprecipitation,RNA sequencing,non-targeted metabolomics,polysome profiling,and luciferase reporter assays were performed to investigate the molecular mechanisms of METTL5 in HCC cells.Results:We discovered that METTL5 drove glucose metabolic reprogramming to promote the proliferation and metastasis of HCC.Mechanistically,upregulation of METTL5 promoted c-Myc stability and thus activated its downstream glycolytic genes lactate dehydrogenase A(LDHA),enolase 1(ENO1),triosephosphate isomerase 1(TPI1),solute carrier family 2 member 1(SLC2A1),and pyruvate kinase M2(PKM2).The c-Box and ubiquitin binding domain(UBA)regions of ubiquitin specific peptidase 5(USP5)binded to c-Myc protein and inhibited K48-linked polyubiquitination of c-Myc.Further study revealed that METTL5 controled the USP5 translation process,which in turn regulated the ubiquitination of c-Myc.Furthermore,we identified cAMP responsive element binding protein 1(CREB1)/P300 as a critical transcriptional regulator ofMETTL5 that promoted the transcription of METTL5 in HCC.In patient-derived tumor xenograft(PDX)m

关键词： METTL5 c-Myc USP5 P300 CREB1 deubiquitination hepatocellular carcinoma glucose metabolism

glucose metabolism and Human Immunodeficiency Virus Type 1 Infection

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Infectious Diseases & Immunity 2022年第4期2卷 242-247页

作者： Zhonghe Chen Tiantian Wang Kai Deng Institute of Human Virology Key Laboratory of Tropical Disease Control of Ministry of EducationZhongshan School of MedicineSun Yat-sen UniversityGuangzhou 510080China Department of Immunology and Microbiology Zhongshan School of MedicineSun Yat-sen UniversityGuangzhou 510080China

Acquired immune deficiency syndrome is still one of the most severe global infectious diseases that pose a significant threat to human health.With the successful application of antiretroviral therapy,productive replication of human immunodeficiency virus type 1(HIV-1)can be effectively blocked;however,antiretroviral therapy alone cannot cure the infection because of the presence of a stable and reactivatable viral latent reservoir.Thus,it is of great importance to have a better comprehension of themechanisms driving HIV-1 pathogenesis and long-term persistence in infected individuals,based on which to further discover novel targets for therapeutic applications to treat or even cure the infection.Various studies have revealed that cellularmetabolism is a critical factor impacting the fate and intracellular activities of immune cells.Emerging evidence implies that the alternations of cellular metabolism induced by HIV-1 infection play an important role in HIV-1 pathogenesis.Consequently,a promising approach of“metabolism as a therapeutic target”raised the possibility of using metabolic reprogramming as a treatment option for chronic HIV-1 infection.In this review,we summarize the latest studies about the interplay of the hosts'reprogramming of glucose metabolism and HIV-1 infection and introduce potential applications of searching for hallmarks and therapeutic targets of metabolic interventions for HIV-1 infection.

关键词： HIV-1 glucose metabolism Metabolic reprogramming Chronic inflammation

Glucuronic acid metabolites of phenolic acids target AKT-PH domain to improve glucose metabolism

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Chinese Herbal Medicines 2023年第3期15卷 398-406页

作者： Jie Gao Manqian Zhang Xingwang Zu Xue Gu Erwei Hao Xiaotao Hou Gang Bai State Key Laboratory of Medicinal Chemical Biology College of Pharmacy and Tianjin Key Laboratory of Molecular Drug ResearchNankai UniversityTianjin 300353China Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica Guangxi Collaborative Innovation Center for Research on Functional Ingredients of Agricultural ResiduesGuangxi University of Chinese MedicineNanning 530200China

Objective:Phenolic acids widely exist in the human diet and exert beneficial effects such as improving glucose metabolism.It is not clear whether phenolic acids or their metabolites play a major role in vivo.In this study,caffeic acid(CA)and ferulic acid(FA),the two most ingested phenolic acids,and their glucuronic acid metabolites,caffeic-4’-O-glucuronide(CA4G)and ferulic-4’-O-glucuronide(FA4G),were investigated.Methods:Three insulin resistance models in vitro were established by using TNF-a,insulin and palmitic acid(PA)in HepG2 cells,respectively.We compared the effects of FA,FA4G,CA and CA4G on glucose metabolism in these models by measuring the glucose consumption levels.The potential targets and related pathways were predicted by network pharmacology.Fluorescence quenching measurement was used to analyze the binding between the compounds and the predicted target.To investigate the binding mode,molecular docking was performed.Then,we performed membrane recruitment assays of the AKT pleckstrin homology(PH)domain with the help of the PH-GFP plasmid.AKT enzymatic activity was determined to compare the effects between the metabolites with their parent compounds.Finally,the downstream signaling pathway of AKT was investigated by Western blot analysis.Results:The results showed that CA4G and FA4G were more potent than their parent compounds in increasing glucose consumption.AKT was predicted to be the key target of CA4G and FA4G by network pharmacology analysis.The fluorescence quenching test confirmed the more potent binding to AKT of the two metabolites compared to their parent compounds.The molecular docking results indicated that the carbonyl group in the glucuronic acid structure of CA4G and FA4G might bind to the PH domain of AKT at the key Arg-25 site.CA4G and FA4G inhibited the translocation of the AKT PH domain to the membrane,while increasing the activity of AKT.Western blot analysis demonstrated that the metabolites could increase the phosphorylation of AKT and downstream glycogen synthase kinase 3βin th

关键词： AKT glucuronic acid glucose metabolism metabolite phenolic acid PH domain