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Characterization of the Formation of Branched Short-Chain Fatty Acid：CoAs for Bitter Acid Biosynthesis in Hop glandular trichomes

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Molecular Plant 2013年第4期6卷 1301-1317页

作者： Haiyang Xu Fengxia Zhang Baoxiu Liu David V. Huhman Lloyd W. Sumner Richard A. Dixon Guodong Wang State Key Laboratory of Plant Genomics and National Center for Plant Gene Research Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China University of Chinese Academy of Sciences Beijing 100039 China The Samuel Roberts Noble Foundation Plant Biology 2510 Sam Noble Parkway Ardmore OK 73401 USA Current address: Department of Biological Sciences University of North Texas Denton Texas 76203 USA

Bitter acids, known for their use as beer flavoring and for their diverse biological activities, are predominantly formed in hop （Humulus lupulus） glandular trichomes. Branched short-chain acyI-CoAs （e.g. isobutyryI-CoA, isovaleryl- CoA and 2-methylbutyryI-CoA）, derived from the degradation of branched-chain amino acids （BCAAs）, are essential building blocks for the biosynthesis of bitter acids in hops. However, little is known regarding what components are needed to produce and maintain the pool of branched short-chain acyI-CoAs in hop trichomes. Here, we present several lines of evidence that both CoA ligases and thioesterases are likely involved in bitter acid biosynthesis. Recombinant HICCL2 （carboxyl CoA ligase） protein had high specific activity for isovaleric acid as a substrate （Kcat/Km = 4100 s-~ M-l）, whereas recombinant HICCL4 specifically utilized isobutyric acid （Kcat/Km = 1800 s-1 M-1） and 2-methylbutyric acid （Kcat/ Km = 6900 s-1 M-~） as substrates. Both HICCLs, like hop valerophenone synthase （HIVPS）, were expressed strongly in glandular trichomes and localized to the cytoplasm. Co-expression of HICCL2 and HICCL4 with HIVPS in yeast led to significant production of acylphloroglucinols （the direct precursors for bitter acid biosynthesis）, which further confirmed the biochemical function of these two HICCLs in vivo. Functional identification of a thioesterase that catalyzed the reverse reaction of CCLs in mitochondria, together with the comprehensive analysis of genes involved BCAA catabolism, supported the idea that cytosolic CoA ligases are required for linking BCAA degradation and bitter acid biosynthesis in glandular trichomes. The evolution and other possible physiological roles of branched short-chain fatty acid：CoA ligases in planta are also discussed.

关键词： CoA ligase glandular trichomes bitter acid thioesterase evolution Humulus lupulus

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Terpenophenolics Biosynthesis in glandular trichomes from Hop(Humulus lupulus L.)

Terpenophenolics Biosynthesis in Glandular Trichomes from Ho...

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中国化学会第十二届全国天然有机化学学术会议

作者： Zhaonan Ban Hao Qin Haoxun Li Haiyang Xu Baoxiu Liu Fengxia Zhang 王国栋 State Key Laboratory of Plant Genomics and National Center for Plant Gene Research Institute of Genetics and Developmental BiologyChinese Academy of Sciences

Hop(Humulus lupulus ***) is an important commodity in the beer brewing industry,mostly due to its high content of terpenophenolics(a mixture of xanthohumol and bitter acids) in hop glandular *** are considerable researches for potential health benefits of hop *** this study,we firstly elucidate the biosynthetic pathway for hop terpenophenolics,and explained the high efficiency of terpenophenolics biosynthesis in hop glandular trichomes at molecular *** finally reconstruct the whole terpenophenolics pathways in yeast cells to produce these health-beneficial natural products in large scale.

关键词： Humulus lupulus L. glandular trichomes Terpenophenolics

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Cloning and functional characterization of three monoterpene synthases from Artemisia annua

Cloning and functional characterization of three monoterpene...

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第十四届全国植物基因组学大会

作者： Juxin Ruan Jianxu Li Xiaoya Chen National Key Laboratory of Plant Molecular Genetics and National Plant Gene Research Center Institute of Plant Physiology and EcologyShanghai Institutes for Biological SciencesChinese Academy of Sciences

From a cDNA library of Artemisia annua glandular trichomes,six cDNAs encoding putative terpene synthases(TPSs) were *** of them,AaTPS2,AaTPS5 and AaTPS6,appeared to encode monoterpene synthases containing a serine and... 详细信息

关键词： monoterpenes TPS Artemisia annua glandular trichomes

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Characterization of a Cotton Fiber Gene Promoter

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棉花学报 2008年第S1期20卷 67-页

作者： SHANGGUAN Xiao-xia,XU Bing,YU Zong-xia,WANG Ling-jian,CHEN Xiao-ya(National Key Laboratory of Plant Molecular Genetics,Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200032,China) National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and EcologyShanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200032China

Cotton fibers are unicellular trichomes derived from outer integument cells of the *** previously study showed that cotton R2R3 MYB transcript factor GaMYB2 could complement the Arabidopsis trichome mutant of glabra1(... 详细信息

关键词： promoter cotton fiber trichome glandular trichomes MYB

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