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Gene expression profiling in porcine mammary gland.during lactation and identification of breed- and developmental-stage-specific genes

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Science China(Life Sciences) 2006年第1期49卷 26-36页

作者： SU Zhixi1, DONG Xinjiao2, ZHANG Bing1, ZENG Yanwu1, FU Yan1, YU Jun1,3 & HU Songnian1,3 1. James D. Watson Institute of Genome Sciences, Zhejiang University, Hangzhou 310008, China 2. School of Life and Environmental Sciences, Wenzhou Normal College, Wenzhou 325027, China 3. Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 101300, China 1. James D. Watson Institute of Genome Sciences Zhejiang University Hangzhou 310008 China 2. School of Life and Environmental Sciences Wenzhou Normal College Wenzhou 325027 China 3. Beijing Institute of Genomics Chinese Academy of Sciences Beijing 101300 China

A total of 28941 ESTs were sequenced from five 5′-directed non-normalized cDNA libraries, which were assembled into 2212 contigs; 5642 singlets using CAP3. These sequences were annotated; clustered into 6857 unique genes, 2072 of which having no functional annotations were considered as novel genes. These genes were further classified into Gene Ontology categories. By comparing the expression profiles, we identified some breed-and developmental-stage-specific gene groups. These genes may be relative to reproductive performance or play important roles in milk synthesis, secretion; mammary involution. The unknown EST sequences; expression profiles at different developmental stages; breeds are very important resources for further research.

关键词： expressed sequence tags, expression profile, domestic pig, mammary gland.

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Construction of transgenic mice producing CAT to milk by co-injection of two overlapping fragments of bovine αs1-casein-CAT gene

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Science China(Life Sciences) 1996年第6期39卷 592-599页

作者：劳为德刘伟成国祥徐少甫成勇 Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China Jiangsu Agricultural College Yangzhou University Yangzhou 225001 China

Two αs1-casein/chloramphenicol acetyltransferase (CAT) gene constructs overlapping by 3.0kb were constructed and co-injected into murine zygotes. In 9 of 10 lines of transgenic mice obtained, based on analysis of structure and expression of the transgene, accurate extrachromosomal homologous recombination (ECR) between the two overlapping DNA fragments was found. Different levels of CAT activity were detected in milk from these lines. The highest CAT activity was about 25-50μg/mL milk. In some mice. CAT activity was found in salvia gland. thymus and spleen extracts. The high frequency and accuracy of ECR reported here will be applicable in the experimental manipulation for generation of relatively large transgene.

关键词： extrachromosomal homologous recombination bovine a si-casein gene chloramphenicol acetyltransferase transgenic mice mammary gland.

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High expression of human serum albumin in milk of trans-genie mice directed by the goat β-casein gene promoter region

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Chinese Science Bulletin 2001年第7期46卷 582-586页

作者： HUANG Ying, HUANG Ying, HUANG Zan,YAN Jingbin, MA Zhanlu, SHENG Min,REN Zhaorui, ZENG Yitao & HUANG ShuzhenShanghai Institute of Medical Genetics, Shanghai Children’s Hospital, Shanghai 200040, China

We have constructed a mammary gland.expression vector that contained the goat β-casein gene pro-moter, 5’upstream regulatory region, exons 1, 2, intron 1 as well as the human serum albumin (hALB) mini-gene (including the full-long sequences of hALB cDNA and its intron 1). Injection of the vector into mouse tail veins showed that the recombinant construct was expressed only in mammary gland.. The vector was microinjected into the mouse fertilized eggs, followed by transferring the eggs into the foster mice. 33 F0 mice were obtained. Of the 33, 8 mice (5 , 3 ) were transgenic with hALB gene integration identified by PCR as well as Southern blot hybridization. The integration rate was 24.2% (8/33). Western blot analysis showed that 3 female transgenic mice had hALB expression in their milk. The hALB contents in milk reached 3.54, 0.21 and 3.03 g/L, respectively.

关键词： human serum albumin goat β-casein transgenic mice mammary gland.

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