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Microarray and Proteomic Analysis of Brassinosteroid-and gibberellin-Regulated Gene and Protein Expression in Rice

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Genomics, Proteomics & Bioinformatics 2004年第2期2卷 77-83页

作者： Setsuko Komatsu Department of Molecular Genetics National Institute of Agrobiological Sciences Tsukuba 305-8602 Japan.

Brassinosteroid (BR) and gibberellin (GA) are two groups of plant growthregulators essential for normal plant growth and development. To gain insight into the molecularmechanism by which BR and GA regulate the growth and development of plants, especially the monocotplant rice, it is necessary to identify and analyze more genes and proteins that are regulated bythem. With the availability of draft sequences of two major types, japonica and indica rice, it hasbecome possible to analyze expression changes of genes and proteins at genome scale. In this review,we summarize rice functional genomic research by using microarray and proteomic approaches and ourrecent research results focusing on the comparison of cDNA microarray and proteomic analyses of BR-and GA-regulated gene and protein expression in rice. We believe our findings have importantimplications for understanding the mechanism by which BR and GA regulate the growth and developmentof rice.

关键词： brassinosteroid gibberellin microarray proteome rice

Functional Characterization of gibberellin-Regulated Genes in Rice Using Microarray System

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Genomics, Proteomics & Bioinformatics 2006年第3期4卷 137-144页

作者： Asad Jan Setsuko Komatsu National Institute of Agrobiological Sciences Tsukuba 305-8602 Japan National Institute of Agrobiological Sciences Tsukuba 305-8602 Japan National Institute of Crop Science Tsukuba 305-8518 Japan.

gibberellin （GA） is collectively referred to a group of diterpenoid acids, some of which act as plant hormones and are essential for normal plant growth and development. DNA microarray technology has become the standard tool for the parallel quantification of large numbers of messenger RNA transcripts. The power of this approach has been demonstrated in dissecting plant physiology and development, and in unraveling the underlying cellular signaling pathways. To understand the molecular mechanism by which GA regulates the growth and development of plants, with reference to the monocot model plant-rice, it is essential to identify and analyze more genes and their products at the transcription and translation levels that are regulated by GA. With the availability of draft sequences of two major rice types, indica and japonica rice, it has become possible to analyze global expression profiles of genes on a genome scale. In this review, the progress made in finding new genes in rice leaf sheath using microarray system and their characterization is discussed. It is believed that the findings made in this regard have important implications for understanding the mechanism by which GA regulates the growth and development of rice.

关键词： gibberellin gene expression microarray rice

PROTEOME ANALYSIS OF RICE ROOT PROTEINS REGULATED BY gibberellin

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Genomics, Proteomics & Bioinformatics 2005年第3期3卷 132-142页

作者： SETSUKO KOMATSU HIROSATO KONISHI DEPARTMENT OF MOLECULAR GENETICS NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES TSUKUBA 305-8602 JAPAN

To gain an enhanced understanding of the mechanism by which gibberellins （GAs） regulate the growth and development of plants, it is necessary to identify proteins regulated by GA. Proteome analysis techniques have been applied as a direct, effective, and reliable tool in differential protein expressions. In previous studies, sixteen proteins showed differences in accumulation levels as a result of treatment with GA3, uniconazole, or abscisic acid （ABA）, and/or the differences between the GA-deficient semi-dwarf mutant, Tan-ginbozu, and normal cultivars. Among these proteins, aldolase increased in roots treated with GA3, was present at low levels in Tan-ginbozu roots, and decreased in roots treated with uniconazole or ABA. In a root elongation assay, the growth of aldolase-antisense transgenic rice was half of that of vector control transgenic rice. These results indicate that increases in aldolase activity stimulate the glycolytic in the GA-induced growth of roots. In among GA, aldolase, and root growth. pathway and may play an important role this review, we discuss the relationship among GA, aldolase, and root growth.

关键词： aldolase gibberellin proteome rice

Transcriptional Responses to gibberellin and Abscisic Acid in Barley Aleurone

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Journal of Integrative Plant Biology 2006年第5期48卷 591-612页

作者： Kegui Chen Yong-Qiang Charles An Department of Agronomy University of Wisconsin Madison Wl 53706 USA United States Department of Agriculture Agricultural Research Service Cereal Crops Research Madison Wl 53726 USA

Cereal aleurone has been established as a model system to investigate giberrellin （GA） and absclslc acid （ABA） responses. Using Barley 1 GeneChip, we examined the mRNA accumulation of over 22 000 genes In de-embryonated barley aleurone treated with GA and ABA. We observed that 1 328 genes had more than a threefold change In response to GA treatment, whereas 206 genes had a more than threefold change in response to ABA treatment. Interestingly, approximately 2.5-fold more genes were up-regulated than downregulated by ABA. Eighty-three genes were differentially regulated by both GA and ABA. Most of the genes were subject to antagonistic regulation by ABA and GA, particularly for genes related to seed maturation and germination, such as genes encoding late embryogenesis abundant proteins and storage mobilization enzymes. This supports the antagonistic roles of GA and ABA in seed maturation and seed germination. Interestingly, we observed that a significant percentage of the genes were coordinately regulated by both GA and ABA. Some GA-responsive genes encoded proteins involved in ethylene, Jasmonate, brasslnosterold and auxin metabolic and signaling transducUon pathways, suggesting their potential Interaction with the GA response. We also identified a group of transcription factor genes, such as MYB and Homeobox genes, that were differentially regulated by GA. In addition, a number of GA- and/or ABA-responsive genes encoded components potentially Involved in GA and ABA signal transducUon pathway. Overall, the present study provides a comprehensive and global view of transcript expression accompanying the GA and ABA response in barley aleurone and Identifies a group of genes with potential regulatory functions in GA- and ABA-slgnallng pathways for future functional validation.

关键词： abscisic acid barley gene regulation gibberellin microarray signal transduction.

Brassinosteroid and gibberellin coordinate rice seed germination and embryo growth by regulating glutelin mobilization

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The Crop Journal 2021年第5期9卷 1039-1048页

作者： Min Xiong Lingyi Chu Qianfeng Li Jiawen Yu Yihao Yang Peng Zhou Yong Zhou Changquan Zhang Xiaolei Fan Dongsheng Zhao Changjie Yan Qiaoquan Liu Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Sate Key Laboratory of Hybrid Rice College of AgricultureYangzhou UniversityYangzhou 225009JiangsuChina Co-Innovation Center forModern Production Technology of Grain Crops of Jiangsu Province/Key Laboratory of Plant Functional Genomics of the Ministry of Education Yangzhou UniversityYangzhou 225009JiangsuChina

Seed germination is the beginning of a new lifecycle,and involves many complex physiological and biochemical reactions including seed reserve mobilization in the endosperm and nutrient transport and reuse in the embryo.Although glutelin is a dominant storage protein in rice,its contribution to seed germination and its regulatory mechanisms are mostly unknown.gibberellin (GA) and brassinosteroid (BR),two major growth-promoting phytohormones,also play positive roles in controlling seed germination.However,how GA and BR interact and coordinate seed germination and facilitate glutelin mobilization remains unclear.In the present study,biochemical and physiological analyses of seed germination indicated that both GA and BR promote seed germination and post-germination growth.Exogenous application of GA restored germination defects caused by BR deficiency or insensitivity.Proteomic and q RT-PCR results showed that the expression of several glutelin proteins and their encoding genes was induced by BR and GA in the embryo.Expression assays suggested that the increased accumulation of glutelin protein in the embryo was due to the accelerated degradation of glutelin by a cysteine proteinase (REP-1) in the endosperm.The breakdown of glutelin in the endosperm showed a strict positive correspondence with the length of the shoot.The GluA2 mutation led to reduced degradation rate of glutelin and defects in seed germination,and the promotion effect of GA on seed germination was weakened in the glua2mutant.In vitro culture assay of rice embryos showed that glutelin mobilization functioned downstream of the GA and BR pathways to promote shoot elongation.These findings suggest a mechanism that mediates crosstalk between BR and GA in co-regulating rice seed germination and embryo growth.

关键词： Seed germination Glutelin mobilization Brassinosteroid gibberellin Oryza sativa L.

Exogenous Application of Abscisic Acid or gibberellin Acid Has Different Effects on Starch Granule Size Distribution in Grains of Wheat

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Journal of Integrative Agriculture 2013年第9期12卷 1551-1559页

作者： PENG Dian-liang CAI Tie YIN Yan-ping YANG Wei-bing NI Ying-li YANG Dong-qing WANG Zhen-lin National Key Laboratory of Crop Biology Shandong Agricultural University

Granule size distribution of wheat starch is an important characteristic that can affect its chemical composition and the functionality of wheat products. Two high-yield winter wheat cultivars were used to evaluate the effects of the application of exogenous ABA or GA during the reproductive phase of the initial grain filling on starch granule size distribution and starch components in grains at maturity. The results indicated that a bimodal curve was found in the volume and surface area distribution of grain starch granules, and a unimodal curve was observed for the number distribution under all treatments. The exogenous ABA resulted in a significant increase in the proportions （both by volume and by surface area） of B-type （〈9.9 Ixm in diameter） starch granules, with a reduction in those of A-type （〉9.9 ~tm） starch granules, while, the exogenous GA3 led to converse effects on size distribution of those starch granules. The exogenous ABA also increased starch, amylose and amylopectin contents at maturity but significantly reduced the ratio of amylose to amylopectin. Application of GA3 significantly reduced starch content, amylopectin content but increased the ratio of amylose to amylopectin. The ratio of amylose to amylopectin showed a significant and negative relationship with the volume proportion of granules 〈9.9 μm, but was positively related to the volume proportion of granules 22.8-42.8 μm.

关键词： abscisic acid gibberellin granule size distribution starch winter wheat （Triticum aestivum L.）

Effect of Exogenous gibberellin on Wheat Seed Germination under Salt Stress

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Asian Agricultural Research 2023年第8期15卷 63-66页

作者： Tureding Abudurousuli Baotong LI Aizemaiti Tuerhong Hetian Vocational and Technical College Hetian 848000China

[Objectives]In this experiment,wheat seeds were treated with different concentrations of gibberellin and different concentrations of salt solution to study the change of germination index of wheat seeds.[Methods]The germination rate,germination potential and germination index of wheat seeds were measured by routine methods,and the effect of exogenous gibberellin on germination of wheat seeds under salt stress was observed.[Results]The germination rate,germination potential and germination index of wheat seeds under salt stress were significantly increased after exogenous treatment of 0.25 and 0.50 g/L gibberellin within the range of salt concentration gradient.However,when the concentration of gibberellin was too high,it would inhibit the germination of seeds.[Conclusions]Appropriate concentration of gibberellin can effectively alleviate the stress caused by salt on wheat seed germination.In this experiment,the best concentration of gibberellin to alleviate salt stress was 0.25 g/L.

关键词： gibberellin Salt stress Wheat seed Seed germination

Effects of Brassinosteroid Associated with Auxin and gibberellin on Apple Tree Growth and Gene Expression Patterns

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Horticultural Plant Journal 2019年第3期5卷 93-108页

作者： Liwei Zheng Cai Gao Caide Zhao Lizhi Zhang Mingyu Han Na An Xiaolin Ren College of Horticulture Northwest A&F University College of Life Science Northwest A & F University

Brassinosteroid is an important hormone that interacts with auxin and gibberellin to regulate plant growth and development. However,there is currently a relative lack of information regarding brassinosteroid in apple. Previous study confirmed that exogenous brassinosteroid treatments increased growth and endogenous brassinosteroid, auxin, as well as gibberellin levels of apple nursery trees. Here we succeeded to find that exogenous brassinosteroid treatments upregulated the transcript expression of auxin biosynthesis, transport, and positive signal transduction genes as well as gibberellin biosynthesis genes. In contrast, the application of exogenous brassinosteroid downregulated the expression of genes encoding negative regulators of auxin and gibberellin signal transductions. Rapid responses of several brassinosteroid-,auxin-, and gibberellin-related genes to the brassinosteroid and brassinazole treatments inferred the crosstalk of BR and IAA or GA. Furthermore,the expression levels of cell growth-related genes were also enhanced by exogenous brassinosteroid. Auxin and gibberellin treatments also influenced growth of apple tree and enhanced the expression of brassinosteroid-and cell growth-related genes. These results indicate that the growth of apple tree is regulated by the interaction between brassinosteroid, auxin, and gibberellin. Our work opened new avenues for deep portfolio of apple tree growth and laid the foundation for future studies aimed at elucidating the mechanisms regulating hormone interactionsmediated growth in apple trees.

关键词： Malus domestica Brassinosteroid Auxin gibberellin Growth and development Gene expression

Seed Germination Traits of Loquat (Eriobotrya japonica Lindl.) as Affected by Various Pre-Sowing Treatments (Cutting of Cotyledons, Removal of Perisperm, Moist Chilling and/or Exogenous Application of gibberellin)

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Phyton-International Journal of Experimental Botany 2020年第3期89卷 645-656页

作者： Thiresia-Teresa Tzatzani Evangelia Basdeki Evangelia-Vasiliki Ladikou Marios-Ioannis NSotiras Georgios Panagiotakis Sryridon Lionakis Ioannis E.Papadakis Laboratory of Subtropical Plants and Tissue Culture Institute of Olive TreeSubtropical Plants and ViticultureHellenic Agricultural Organization-DEMETERChania73134Greece Laboratory of Genetics Department of BiotechnologyAgricultural University of Athens(Member of the European University for Smart Urban Coastal Sustainability-EU-CONEXUS)Athens11855Greece Laboratory of Pomology Department of Crop ScienceAgricultural University of Athens(Member of the European University for Smart Urban Coastal Sustainability-EU-CONEXUS)Athens11855Greece Laboratory of Pomology School of Agricultural SciencesHellenic Mediterranean UniversityHeraklion71410Greece

The purpose of this study was to investigate the effects of various presowing treatments on the germinability(final germination percentage)and germination rate of loquat seeds in order to increase seedling production in nurseries(applied research)as well as provide answers for important physiological issues related to loquat seeds and their seed coat(basic research).Three experiments were carried out with various pre-sowing treatments.These treatments included full or partial removal of seed coat(perisperm),partial cutting of cotyledons as well as moist chilling at 5℃ for 13 days and/or soaking the seeds in water or 250 ppm gibberellic acid(GA_(3))solution for 24 h.According to the results,cotyledons excision resulted in delayed germination,regardless of the presence or absence of the seed coat in comparison with the decoated seeds that demonstrated the highest germination rate amongst them.In addition,even the partial excision of seed coats affected positively both the germinability and the germination rate,compared to the control-intact seeds.Furthermore,control-intact seeds had a higher germination percentage when exposed to moist chilling independently of the application or not of gibberellin;while the combination of gibberellin application and moist chilling improved both the percentage and the rate of germination of decoated seeds.In conclusion,the role of perisperm(seed coat)in the germination procedure of loquat seeds seems to be important,indicating the existence of seed coat-imposed dormancy on loquat seeds.Finally,the existence of a mild endogenous embryo-dormancy on loquat is also discussed.

关键词： Cotyledon gibberellin seed coat seed testa Eriobotrya japonica plant propagation perisperm sexual propagation seedling dormancy