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germacrone Induces Apoptosis in Human Hepatoma HepG2 Cells through Inhibition of the JAK2/STAT3 Signalling Pathway

Journal of Huazhong University of Science and Technology(Medical Sciences) 2013年第3期33卷 339-345页

作者：柳昀熠郑倩方斌王维马凤云 Sadia Roshan Amal Banafa 陈明洁常俊丽邓小敏李克秀杨广笑何光源 The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology Chinese National Center of Plant Gene Research (Wuhan) HUST PartKey Laboratory of Molecular Biophysics of Chinese Ministry of EducationCollege of Life Science and TechnologyHuazhong University of Science and Technology

Summary： Previous studies have shown that STAT3 plays a vital role in the genesis and progression of cancer. In this study, we investigated the relationship between the JAK2/STAT3 signalling pathway and germacrone-induced apoptosis in HepG2 cells. HepG2 cells were incubated with germacrone for 24 h, the protein expression of p-STAT3, STAT3, p-JAK2 and JAK2 was detected by Westem Blotting, and RT-PCR was used to determine the expression of STAT3, p53, Bcl-2 and Bax at transcriptional levels. Besides that, HepG2 cells were pre-treated with AG490 or IL-6 for 2 h, and then incubated with ger- macrone for 24 h. The expression ofp-JAK2, JAK2, p-STAT3, STAT3, p53, Bax and Bcl-2 was detected by Western blotting. The activity of HepG2 cells was tested by MTT assay. The apoptosis of HepG2 cells and levels of reactive oxygen species （ROS） were flow cytometrically measured. The results showed that germacrone exposure decreased p-STAT3 and p-JAK2 and regulated expression of p53 and Bcl-2 family members at the same time. Moreover, IL-6 enhanced the activation of the JAK2/STAT3 signalling pathway and therefore attenuated the germacrone-induced apoptosis. Suppression of JAK2/STAT3 signalling pathway by AG490, an inhibitor of JAK2, resulted in apoptosis and an increase in ROS in response to germacrone exposure. We therefore conclude that germacrone induces apoptosis through the JAK2/STAT3 signalling pathway.

关键词： germacrone JAK2/STAT3 apoptosis HepG2 ceils

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High-Performance Liquid Chromatographic Method for Determination of germacrone in Rat Plasma

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Journal of Chinese Pharmaceutical Sciences 2004年第3期13卷 190-192页

作者： HEHai-bing TANGXing CUIFu-de SchoolofPharmacy ShenyangPharmaceuticalUniversityShenyang110016China

Aim A reliable high-performance liquid chromatographic (HPLC) method wasdeveloped for determination of germacrone in rat plasma. Methods The plasma samples were treatedwith acetonitrile and analyzed by HPLC with UV detection at 244 nm. Results The limit of detectionwas 100 ng·mL^(-1) for germacrone in plasma and the linear range was 0.1004-15.06 μg·mL^(-1) inplasma. The RSD of intra-day and inter-day assay was 1.87% - 4.29% and 1.29% -5.15%, *** recoveries of germacrone were over 95%. The endogenous substances in plasma did not show anyinterference in the analysis. Conclusion The method is accurate and convenient, and suitable forpharmacokinetic studies of germacrone in rats.

关键词： high-performance liquid chromatography germacrone volatile oil of rhizomacurcumae

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