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Analysis on genotypic differentiation of Phytophthora infestans by Using Random Amplified Polymorphic DNA (RAPD)

Journal of Northeast Agricultural University(English Edition) 2010年第2期17卷 7-14页

作者： ZHANG Xuanzhe,and XU Shengjun College of Agriculture,Northeast Agricultural University,Harbin 150030,China College of Agriculture Northeast Agricultural UniversityHarbin 150030China

A total of 104 isolates including two Korean isolates and three Japanese isolates of Phytophthora infestans collected from Heilongjiang and Jilin Provinces from 2006 to 2008 were used to determine their mating types,metalaxyl resistance,and RAPD *** the isolates of *** collected from Heilongjiang and Jilin Provinces belonged to A 1 mating type,and no A 2 mating type was *** of metalaxyl resistant isolates were 94.4%,47.8% and 75.0% in 2006,2007 and 2008,***...

关键词： Phytophthora infestans genotypic differentiation RAPD

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Analysis of Population Genetic Change to Single Oospore Strains of Phytophthora infestans

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Journal of Northeast Agricultural University(English Edition) 2023年第2期30卷 20-31页

作者： Zhang Xuan-zhe Xu Hao-ran Zhu Er-long Li Yuan-yuan Chen Su-hui Qian Bao-hong Ai Xin Pang Lin College of Plant Protection Northeast Agricultural UniversityHarbin 150030China

The occurrence of sexual reproduction accelerates the population genetic variation of Phytophthora infestans and makes it more difficult to *** systematic analysis of the differentiation of phenotype(mating type and metalaxyl sensitivity)and genotype(mtDNA haplotype and SSR genotype)of 65 single oospore strains of *** was carried out in this *** test strains were isolated from Heilongjiang Province and Mongolia Autonomous *** experiment results showed that the isolation ratio of metalaxyl resistance(MR:HR)of single oospore strains produced through the cross of medium resistance and high resistant parents was 18:13;the isolation ratio of the metalaxyl resistance(S:MR:HR)of single oospore strains produced through the cross of sensitive and high resistant parents was 4:12:*** progenies of single oospore strains produced through self-fertility parents with medium resistance were all of the medium *** mating types A1:A2 was greater than 1:1 in single oospore strains of the progenies,which did not conform to the Mendel's law of *** single oospore strains of the progenies inherited mitochondrial DNA fragments from only one *** recombination of single oospore strains was verified by using two pairs of SSR primers(Pi4B and Pi4G).At the locus of Pi4B and Pi4G in the cross of KS-37 and KS-25,the separation frequencies of allele were 19:12 and 14:17,*** produced two new genotype *** study could provide a basis for formulating disease control strategies.

关键词： Phytophthora infestans single oospore strain phenotypic differentiation genotypic differentiation

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