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genetic Diversity of Three Aristichthys nobilis Populations and One Inbreeding Stock

Wuhan University Journal of Natural Sciences 2002年第2期7卷 243-247页

作者： Wang He Deng Feng-jiao Yu Lai-ning Fang Yao-ling Zhang Xi-yuan Xiang fang College of Life Sciences Wuhan University Wuhan 430072HubeiChina Yangtse River of Fisheries Institute Jingzhou 434000Hub

genetic diversity of threeAristichthys nobilis populations and one inbreeding stock was studied using random amplified polymorphic DNA (RAPD) method. Materials of natural populations were collected from two counties of Guangxi Province, Hengxian (NH) and Shanglin (NS) and one city of Hubei Province, Wuhan city. (NW). Shanglin and Hengxian's river system respectively belongs to Qianjiang and Yujiang River, two tributaries confluencing to Xunjiang River, main tributary of Xijiang River, which is the biggest tributary of the Zhujiang River system, and Wuhan's water system belongs to the Yangtse River system. The inbreeding stock (RS) was the more than 10 generation descendant by brother-sister mating system whose parents were collected from Shanglin. The results showed that the genetic variety of individuals of RS was very low (0.105 4), whereas that of natural populations was relatively high, which from high to low was 0.158 1 (NW), 0.132 0 (NH) and 0.110 5 (NS). As an index for genetic distance pair-wise populations, the genetic variety between populations was studied, which characterized the genetic distance between populations. The genetic distance of NW and NH, NW and NS, NW and RS were respectively high, whereas that of NH and NS, NS and RS, NH and RS was low. Chi-test (χ 2) and the analysis of the genetic variety pair-wise populations was taken as efficient approach for studying population difference.

关键词： Aristichthys nobilis RAPD genetic variety genetic diversity genetic distance

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genetic analysis of five Porphyra species by isozyme markers

Genetic analysis of five Porphyra species by isozyme markers

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中国海洋湖沼学会藻类学分会第七届会员大会暨第十四次学术...

作者： Shen Songdong~(1+) Xu Pu~2 Zhang Meiru~3 Yuan Zhaolan~1 Wang Jianwei~1 Zhu Jianyi~2 1.Life Sciences College,Suzhou University,Suzhou City,Jiangsu Province,China,215123 2.Department of Biology and Foodstuff engineering,Changshu Institute of Technology,Suzhou City,Jiangsu Province,China,215500 3.Marine Fisheries Institute of Jiangsu Province,Nantong City,Jiangsu Province,China,226007

＜正＞Vertical polylamide gel eletrophoresis was used to investigate isozyme polymorphisms among different isolates（including wild and cultivated）of Porphyra katadai,Porphyra oligospermatangia, Porphyra yezoensis,Porphyra haitanensis,and a hybridize species（Porphyra yezoensis×Porphyra haitanensis）sampled from ***,the analyses of probable minimum loci numbers and observed alleles sum,genetic diversity,and unweighted pair-group arithmetic average（UPGMA）cluster were carried *** initial activity and resolution testing of bands of 23 enzymes,6 of them（MDH, ME,LDH,GDH,IDH and G-6-PDH）proved to be appropriate for analysis of the full sample set The probable minimum numbers of loci and alleles analyses showed that all the five species of Porphyra had an extraordinary consistant result in ME loci and ***,*** and *** differed from other three species of Porphyra in LDH and GDH loci and alleles,while *** was independent in the analyses ofMDH,*** and *** differed from other three species in IDH analyses,while *** and *** were apart from other three species in G-6-PDH *** one with another,*** was most detached in the probable minimum numbers of loci and alleles *** results of genetic diversity analysis indicate the genetic variation between the five Porphyra species studied was limited as a genetic identity of *** hybridize species（*** x ***）seemed to be the most homologue with P. oligospermatangia as got a genetic identity of 0.8937,unexpectedly got relatively lower average genetic identities with both *** and *** 4 strains of *** were relatively more divergent with an average genetic identity of 0.7428,and *** presented most differentiated, branching from all the other species,which consistent with the result summarized in the probable minimum numbers of loci and alleles analyses.

关键词： Porphyra isozyme allele gene locus genetic variety

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