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2021年中国肿瘤标志物学术大会暨第十五届肿瘤标志物青年科学家论坛

作者： Ming Yu Yajuan Su Harbin Medical University Hospital Cancer Hospital Harbin Medical University Cancer Hospital

Objective: Ovarian cancer is the most fatal gynecological malignant tumor, and it is particularly important for the early diagnosis of ovarian cancer. However, the lack of specific tumor markers has become a major problem in the early diagnosis of ovarian cancer. There is increasing evidence that abnormal methylation of gene promoter plays an important role in the carcinogenic process and can silence gene expression, which is an early event in the pathogenesis of ovarian cancer. The gene promoter methylation found in tumor can be reflected in circulating cell-free DNA(cfDNA) released from tumor to peripheral blood, which is consistent with primary tumor, making cfDNA methylation a noninvasive molecular marker based on blood detection. PRDM2 is closely related to the occurrence and development of tumor, and related research has made great progress, which has the potential as a molecular marker. Therefore, this study will explore the expression and clinical significance of PRDM2 gene promoter methylation in ovarian cancer tumor tissues and plasma ***: The tumor tissues from 56 ovarian cancer patients and 36 normal ovarian tissues were collected admitted to the affiliated Cancer Hospital of Harbin Medical University. The corresponding preoperative plasma samples were collected. The expression of PRDM2 mRNA and protein in ovarian tissues was detected by Real-time quantitative PCR(qPCR) and Western-blot, the status of PRDM2 gene promoter methylation in ovarian tissues was detected by methylation specific PCR(MSP), and the correlation between gene expression and its promoter methylation was analyzed. The status of PRDM2 gene promoter methylation inplasma cfDNA was detected by MSP, and the correlation of PRDM2 gene methylation changes in plasma cfDNA and ovarian tissues was analyzed. Moreover, to explore the relationship between PRDM2 gene promoter methylation and clinicopathological features of ovarian cancer patients, and to evaluate the diagnostic value of PRD

关键词： PRDM2 gene methylation ovarian cancer tumor suppressor gene diagnosis

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Genic C-methylation in Soybean Is Associated with gene Paralogs Relocated to Transposable Element-Rich Pericentromeres

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Molecular Plant 2018年第3期11卷 485-495页

作者： Moaine El Baidouri Kyung Do Kim Brian Abernathy Ying-Hui Li Li-Juan Qiu Scott A. Jackson Center for Applied genetic Technologies University of Georgia 111 Riverbend Road Athens GA 30602 USA Corporate R&D LG Chem LG Science Park 30 Magokjungang 10-ro Gangseo-gu Seoul 07796 Republic of Korea The National Key Facility for Crop gene Resources and genetic Improvement (NFCRI) Institute of Crop Science Chinese Academy of Agricultural SciencesBeijing China These authors contributed equally to this article.

Most plants are polyploid due to whole-genome duplications （WGD） and can thus have duplicated genes. Following a WGD, paralogs are often fractionated （lost） and few duplicate pairs remain. Little attention has been paid to the role of DNA methylation in the functional divergence of paralogous genes. Using high- resolution methylation maps of accessions of domesticated and wild soybean, we show that in soybean, a recent paleopolyploid with many paralogs, DNA methylation likely contributed to the elimination of ge- netic redundancy of polyploidy-derived gene paralogs. Transcriptionally silenced paralogs exhibit partic- ular genomic features as they are often associated with proximal transposable elements （TEs） and are pref- erentially located in pericentromeres, likely due to gene movement during evolution. Additionally, we provide evidence that gene methylation associated with proximal TEs is implicated in the divergence of expression profiles between orthologous genes of wild and domesticated soybean, and within populations.

关键词： soybean paralog gene methylation proximal transposable element methylation spreading

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Silence of HIN-1 expression through methylation of its gene promoter in gastric cancer

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World Journal of Gastroenterology 2011年第4期17卷 526-533页

作者： Yan Gong Ming-Zhou Guo Zhi-Jia Ye Xiu-Li Zhang Yong-Liang Zhao Yun-Sheng Yang Department of Gastroenterology and Hepatology Chinese PLAGeneral Hospital Beijing 100853 China Department of genetics Yale Medical School TACS320 1 Gilbert Street New Haven CT 06520 United States Department of general Surgery Southwest Hospital Third Military Medical University Chongqing 400038China

AIM: To clarify the role of high in normal-1 (HIN-1) gene promoter methylation during gastric cancer development. METHODS: Gastric cancer cell lines and tissue specimens were analyzed for expression of HIN-1 mRNA and protein using the semi-quantitative reverse transcription polymerase chain reaction and immunohistochemistry. The methylation of the HIN-1 gene promoter was detected in gastric carcinoma cells and tissues using methylation-specific polymerase chain reaction. The 3-(4,5-dimethylthiazol-2yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium cell viability assay and flow cytometry were used to assess the changes in behaviors of gastric cancer cells with or without 5-aza-2’-deoxycytidine treatment. RESULTS: HIN-1 was not expressed in 4 of 5 gastric cancer cell lines. The demethylation reagent 5-aza-2’-deoxycytidine was able to induce or upregulate HIN-1 expression in gastric cancer cell lines, which is associated with reduction of tumor cell viability. Furthermore, methylation of the HIN-1 gene promoter was shown in 57.8% (26/45) of the primary gastric cancer and 42.1% (17/38) of adjacent tissue samples, but was not shown in normal gastric mucosa (0/10). From the clinicopathological data of the patients, methylation of the HIN-1 gene promoter was found to be associated with tumor differentiation (P = 0.000). CONCLUSION: High methylation of HIN-1 gene promoter results in silence of HIN-1 expression in gastric cancer. 5-aza-2’-deoxycytidine reverses HIN-1 methylation and reduces viability of gastric cancer cells.

关键词： High in normal-1 gene methylation 5-aza-2’-deoxycytidine Tumor differentiation Gastric cancer

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methylation of the PCDH8 (Protocadherin-8) gene in gastric cancer

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Journal of Medical Colleges of PLA(China) 2012年第1期27卷 10-19页

作者： Zhang Danjie Che Xiangming Zhao Wei Liao Xinhua Bi Tieqiang Li Haijun Department of general Surgery First Affiliated HospitalMedical CollegeXi'an Jiaotong UniversityXi'an 710061China

Objective: To investigate the methylation status of the PCDH8 (Protocadherin-8) gene in gastric cancer tissues and find out the relationship between methylation status of the PCDH8 and clinicopathological features in gastric cancer patients. Methods: We first investigated the methylation status of the PCDH8 (Protocadherin-8) gene in 65 gastric cancer and detected aberrant promoter methylation in gastric cancers; and then analyzed he relationship between methylation status of the PCDH8 and clinicopathological status with SPSS 13.0 software. Results: We first investigated the methylation status of the PCDH8 (Protocadherin-8) gene in 65 gastric cancer and detected aberrant promoter methylation in 36 of 65 (55.4%) gastric cancers. There was no significant difference in the distribution of patients with methylation or unmethylation of PCDH8 in terms of age, sex, tumor size, distant metastasis, or TNM stage. methylation of PCDH8 was significantly correlated to negative pathological lymph node metastasis (P=0.038) and tumor differentiation (P=0.01). These two factors were proved to be of prognostic importance. Conclusion: Methylated PCDH8 seems to have a trend for worse prognosis in gastric cancer. However, a further large series of tumor samples and a longer follow-up period are required to elucidate its potential role.

关键词： Protocadherin 8 gene methylation Gastric cancer

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methylation Characteristics of Perivisceral Fat gene in Obese Rats with Phlegm-dampness Syndrome and the Effect of Wen Dan Decoction

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Digital Chinese Medicine 2019年第2期2卷 72-85页

作者： YANG Hai-Yan YU Song-Ren XIA Xun-Li CHENG Shao-Min WANG Ping College of Traditional Chinese Medicine Jiangxi University of Traditional Chinese MedicineNanchangJiangxi 330000China

Objective To observe the characteristics of gene methylation in obese rats with phlegm-dampness syndrome induced by the high-fat diet, and to study the effect of Wen Dan Decoction on gene methylation after the intervention. Methods methylation sites of genes were detected by the MeDIP-seq method. Bioinformatics method was used to analyze the gene methylation characteristics of obesity with phlegmdampness syndrome and the effect of Wen Dan Decoction. Results (1) There were 3 242 methylation differential loci in dietinduced obesity with phlegm-dampness syndrome, of which 1 243 were down-regulated and 1 999 were up-regulated, involving 1 579 differential genes. GO analysis showed that "offactory receptor activity" and others were enriched. The possible signal pathways involved were "Olfactory transduction""Tuberculosis""Systemic lupus erythematosus" and "Ribosome".(2) After the intervention of Wen Dan Decoction in obesity with phlegmdampness syndrome, 4 046 different methylation loci were obtained, including 1 067 down-regulated loci and 2 979 up-regulated loci, involving 2 068 genes. GO analysis showed that "offactory receptor activity" and others were enriched. These genes involved seven signaling pathways, such as "Metabolic pathways".(3) Between diet-induced obesity with phlegm-dampness syndrome and Wen Dan Decoction intervening obesity with the phlegm-dampness syndrome, 582 common genes of methylation differential genes were obtained. After the intervention of Wen Dan Decoction, the number of GO enrichment items was more than that of obesity with phlegm-dampness syndrome, and even the same GO enrichment items involved more genes. Conclusions The phlegm-dampness syndrome of obesityinduced by diet had the characteristics of gene methylation changes, and the intervention of Wen Dan Decoction could also affect the status of gene methylation. The genes affected by Wen Dan Decoction were closely related to the methylation gene of phlegm-dampness syndrome of obesity-indu

关键词： Obesity Phlegm-dampness syndrome gene methylation Wen Dan Decoction Bioinformatics

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