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laser capture microdissection enables cellular and molecular studies of tooth root development

International Journal of Oral Science 2012年第1期4卷 7-13页

作者： Jian-Xun Sun Orapin V Horst Roger Bumgarner Bryce Lakely Martha J Somerman Hai Zhang State Key Laboratory of Oral Diseases Sichuan University Chengdu China Department of Periodontics University of Washington Seattle WA USA Division of Endodontics Department of Preventive and Restorative Dental SciencesUniversity of California at San FranciscoSan FranciscoCAUSA Department of Microbiology University of WashingtonSeattleWAUSA Department of Urology University of WashingtonSeattleWAUSA National Institute of Arthritis and Musculoskeletal and Skin Diseases National Institutes of HealthBethesdaMDUSA Department of Restorative Dentistry UniversitY of Washington Seattle WA USA

Epithelial-mesenchymal interactions（EMIs） are critical for tooth *** mechanisms mediating these interactions in root formation is not well *** capture microdissection（LCM） and subsequent microarray analyses enable large scale in situ molecular and cellular studies of root formation but to date have been hindered by technical challenges of gaining intact histological sections of non-decalcified mineralized teeth or jaws with well-preserved ***,we describe a new method to overcome this obstacle that permits LCM of dental epithelia,adjacent mesenchyme,odontoblasts and cementoblasts from mouse incisors and molars during root *** this method,we obtained RNA samples of high quality and successfully performed microarray *** differences in gene expression,as well as genes not previously associated with root formation,were *** of gene expression data from microarray with real-time reverse transcriptase polymerase chain reaction（RT-pcr） supported our *** genes include known markers of dental epithelia,mesenchyme,cementoblasts and odontoblasts,as well as novel genes such as those in the fibulin *** conclusion,our new approach in tissue preparation enables LCM collection of intact cells with well-preserved RNA allowing subsequent gene expression analyses using microarray and RT-pcr to define key regulators of tooth root development.

关键词： gene laser capture microdissection microarray pcr root

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