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Gonadectomy affects brain derived neurotrophic factor in rats after chronic constriction nerve injury

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Acta Pharmacologica Sinica 2004年第3期25卷 32-38页

作者： Xin ZHAO,Xin WANG,Shu-yun ZHENG,Jian-guo XU1 School of Life Science,Nanjing University,Nanjing 210093 Department of Anesthesiology,Jinling Hospital,Nanjing 210002,China Department of Anesthesiology Jinling Hospital Nanjing 210002

AIM: To assess the effect of gonadectomy on brain derived neurotrophic factor (BDNF) expression in neuropathic pain. METHODS: Using chronic constriction injury (CCI) model, we detected BDNF mRNA in dorsal root gan- glion and protein content in spinal cord by reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay respectively. The time point we chose was post CCI operation d 0, 3, 7, 14, and 21. RESULTS: After CCI surgery, BDNF mRNA in ipsilateral DRGs was upregulated and reached its maximum on post operation d 7. BDNF protein level in ipsilateral spinal cord was also increased and reached its maximum on post operation d 14. The magnitude of this increase in gonadectomy (GDX) rats was significantly smaller than the GDX-sham rats at each time point. CONCLUSION: Gonadectomy reduced the BDNF increment after CCI surgery. Estrogen may affect nociceptive processing by its effect on BDNF.

关键词： gonadectomy estrogens brain-derived neurotrophic factor gene expression regulation pain chronic constriction injury

Effects of chebulinic acid on differentiation of human leukemia K562 cells

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Acta Pharmacologica Sinica 2004年第2期25卷 105-112页

作者： Zong-chun YI, Zhao WANG2, Hai-xia LI, Ming-jie LIU, Rong-cong WU, Xiao-hui WANG Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China Department of Biological Sciences and Biotechnology Tsinghua University 北京 100084

AIM: To study effects of chebulinic acid on erythroid and megakaryocytic differentiation in K562 cells. METHODS: The benzidine staining method was used to evaluate hemoglobin synthesis; the expression of erythroid specific glycophorin A (GPA) protein and megakaryocytic surface marker CD61 was determined by flow cytometry using fluorescence labeled antibodies; erythroid and megakaryocytic mRNA expression was analyzed by RT-PCR. RESULTS: During erythroid differentiation induced by butyric acid (BA) or hemin, chebulinic acid not only inhib- ited the hemoglobin synthesis of BA- and hemin-treated K562 cells in concentration-dependant manner with IC50 of 4 μmol/L and 40 μmol/L respectively, but also inhibited another erythroid differentiation marker acetylcholinesterase at the concentration of 50 μmol/L in the cells either treated or untreated with each erythroid differentiation inducers, whereas chebulinic acid 50 μmol/L did not change GPA protein expression in these cells significantly. When K562 cells were treated with TPA 50 μg/L for 72 h to induce megakaryocytic differentiation, the presence of chebulinic acid 50 μmol/L slightly provoked the decrease of GPA protein expression induced by TPA. Chebulinic acid did not change the TPA-induced CD61 expression at the same concentration. Chebulinic acid also reduced the mRNA levels of erythroid relative genes including γ-globin, PBGD, NF-E2, and GATA-1 genes in K562 cells either treated or untreated with BA, whereas chebulinic acid upregulated the mRNA levels of GATA-2 transcription factor in these cells. CONCLUSION: Chebulinic acid had inhibitory effect on erythroid differentiation likely through changing transcriptional activation of differentiation relative genes, which suggests that chebulinic acid or other tannins might influence the efficiency of some anti-tumor drugs-induced differentiation or the hematopoiesis processes.

关键词： K562cells chebulinic acid butyrates hemin glycophorin CD61 antigen gene expression regulation celldifferentiation

Transcriptional regulation of the Ambient Temperature Response by H2A.Z Nucleosomes and HSF1 Transcription Factors in Arabidopsis

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Molecular Plant 2017年第10期10卷 1258-1273页

作者： Sandra Cortijo Varodom Charoensawan Anna Brestovitsky Ruth Buning Charles Ravarani Daniela Rhodes John van Noort Katja E. Jaeger Philip A. Wigge The Sainsbury Laboratory University of Cambridge 47 Bateman Street Cambridge CB2 1LR UK Department of Biochemistry Faculty of Science Mahidol University 272 Rama Vl Road Ratchathewi District Bangkok 10400 Thailand Integrative Computational BioScience (ICBS) Center Mahidor University 999 Phuttamonthon 4 Road Salaya Nakhon Pathom 73170 Thailand Biological and Soft Matter Physics Huygens-Kamerlingh Onnes Laboratory Leiden University Niels Bohrweg 2 2333 CA Leiden the Netherlands Medical Research Council Laboratory for Molecular Biology Francis Crick Avenue Cambridge CB2 0QH UK Institute of Structural Biology Nanyang Technical University 50 Nanyang Avenue Singapore 639798 Singapore

Temperature influences the distribution, range, and phenology of plants. The key transcriptional activators of heat shock response in eukaryotes, the heat shock factors （HSFs）, have undergone large-scale gene amplification in plants. While HSFs are central in heat stress responses, their role in the response to ambient temperature changes is less well understood. We show here that the warm ambient temperature transcriptome is dependent upon the HSFA1 clade ofArabidopsis HSFs, which cause a rapid and dynamic eviction of H2A.Z nucleosomes at target genes. A transcriptional cascade results in the activation of multiple downstream stress-responsive transcription factors, triggering large-scale changes to the transcriptome in response to elevated temperature. H2A.Z nucleosomes are enriched at temperature-responsive genes at non-inducible temperature, and thus likely confer inducibility of gene expression and higher responsive dynamics. We propose that the antagonistic effects of H2A.Z and HSF1 provide a mechanism to activate gene expression rapidly and precisely in response to temperature, while preventing leaky transcription in the absence of an activation signal.

关键词： gene expression regulation plant temperature sensing and signaling transcriptomics nucleosome dynamics histone variant H2A.Z heat shock transcription factors

Comparative Analysis of Human genes Frequently and Occasionally Regulated by m^6A Modification

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Genomics, Proteomics & Bioinformatics 2018年第2期16卷 127-135页

作者： Yuan Zhou Qinghua Cui Department of Biomedical Informatics School of Basic Medical Sciences Peking University

The m^6A modification has been implicated as an important epitranscriptomic marker, which plays extensive roles in the regulation of transcript stability, splicing, translation, and localization. Nevertheless, only some genes are repeatedly modified across various conditions and the principle of m^6A regulation remains elusive. In this study, we performed a systems-level analysis of human genes frequently regulated by m^6A modification （m^6Afreq genes） and those occasionally regulated by m^6A modification （m^6Aocca genes）. Compared to the m^6Aocca genes, the m^6Afreq genes exhibit gene importance-related features, such as lower dN/dS ratio, higher protein-protein interaction network degree, and reduced tissue expression specificity. Signaling network analysis indicates that the m^6Afreq genes are associated with downstream components of signaling cascades, high-linked signaling adaptors, and specific network motifs like incoherent feed forward loops. Moreover, functional enrichment analysis indicates significant overlaps between the m^6Afreq genes and genes involved in various layers of gene expression, such as being the microRNA targets and the regulators of RNA processing. Therefore, our findings suggest the potential interplay between m^6A epitranscriptomic regulation and other gene expression regulatory machineries.

关键词： m^6A Epitranscriptome Signaling network gene expression regulation gene importance

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MeCP2:multifaceted roles in gene regulation and neural development

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Neuroscience Bulletin 2014年第4期30卷 601-609页

作者： Tian-Lin Cheng Zilong Qiu Institute of Neuroscience Shanghai Institutes for Biological SciencesChinese Academy of Sciences

Methyl-CpG-binding protein 2 （MeCP2） is a classic methylated-DNA-binding protein, dysfunctions of which lead to various neurodevelopmental disorders such as Rett syndrome and autism spectrum disorder. Initially recognized as a transcriptional repressor, MeCP2 has been studied extensively and its functions have been expanded dramatically in the past two decades. Recently, it was found to be involved in gene regulation at the post-transcriptional level. MeCP2 represses nuclear microRNA processing by interacting directly with the Drosha/DGCR8 complex. In addition to its multifaceted functions, MeCP2 is remarkably modulated by post- translational modifications such as phosphorylation, SUMOylation, and acetylation, providing more regulatory dimensions to its functions. The role of MeCP2 in the central nervous system has been studied extensively, from neurons to glia. Future investigations combining molecular, cellular, and physiological methods are necessary for defining the roles of MeCP2 in the brain and developing efficient treatments for MeCP2-related brain disorders.

关键词： MeCP2 Rett syndrome central nervous system gene expression regulation post-translationalmodification post-transcriptional regulation glia

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Differential expression of cholangiocyte and ileal bile acid transporters following bile acid supplementation and depletion

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World Journal of Gastroenterology 2004年第10期10卷 1440-1446页

作者： N.Sertac Kip Konstantinos N.Lazaridis Anatoliy I.Masyuk Patrick L.Splinter Robert C.Huebert Nicholas F.LaRusso Center for Basic Research in Digestive Diseases Division of Gastroenterology and HepatologyDepartment of Internal MedicineMayo Medical SchoolClinic and FoundationRochesterMN 55905USA

AIM: We have previously demonstrated that cholangiocytes, the epithelial cells lining intrahepatic bile ducts,encode two functional bile acid transporters via alternative splicing of a single gene to facilitate bile acid vectorial transport. Cholangiocytes possess ASBT,an apical sodium-dependent bile acid transporter to take up bile acids,and t-ASBT,a basolateral alternatively spliced and truncated form of ASBT to efflux bile *** hepatocyte and ileal bile acid transporters are in part regulated by the flux of bile acids, the effect of alterations in bile acid flux on the expression of t-ASBT in terminal ileocytes remains ***,we tested the hypothesis that expression of ASBT and t-ASBT in cholangiocytes and ileocytes was regulated by bile acid flux. METHODS: expression of ASBT and t-ASBT message and protein in cholangiocytes and ileocytes isolated from pair- fed rats given control (C) and 1% taurocholate (TCA) or 5% cholestyramine (CY) enriched diets,were assessed by both quantitative RNase protection assays and quantitative *** data obtained from each of the control groups were pooled to reflect the changes observed following TCA and CY treatments with respect to the control diets. Cholangiocyte taurocholate uptake was determined using a novel microperfusion technique on intrahepatic bile duct units (IBDUs) derived from C,TCA and CY fed rats. RESULTS: In cholangiocytes,both ASBT and t-ASBT message RNA and protein were significantly decreased in response to TCA feeding compared to C *** contrast, message and protein of both bile acid transporters significantly increased following CY feeding compared to C *** the ileum,TCA feeding significantly up-regulated both ASBT and t-ASBT message and protein compared to C diet,while CY feeding significantly down-regulated message and protein of both bile acid transporters compared to C *** anticipated from alterations in cholangiocyte ASBT expression,the uptake of taurocholate in microp

关键词： Cholestyramine dosage Ileum Taurocholic Acid Alternative Splicing Animals Bile Ducts Diet Eating Epithelial Cells gene expression regulation Male Organic Anion Transporters, Sodium-Dependent Protein Isoforms Rats Rats, Inbred F344 Symporters

Molecular mechanisms regulating expression and function of transcription regulator “inhibitor of differentiation 3”

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Acta Pharmacologica Sinica 2005年第12期26卷 1409-1420页

作者： Robert Wai-sui LIM 65212 USA Department of Medical Pharmacology and Physiology University of Missouri-Columbia

The transcription factor antagonist inhibitor of differentiation 3(Id3)has beenimplicated in many diverse developmental,physiological and *** expression and function is subjected to many levels of *** review summarizes the current understanding of these mecha-nisms and describes how they might be related to the diverse functions that havebeen attributed to the Id3 *** understanding of these mechanismsshould provide insights towards the development of therapeutic approaches tovarious diseases,including cancer and atherogenesis.

关键词： gene expression regulation inhibitor of differentiation 3 protein transcription factors neoplasm

Impact of endoscopically minimal involvement on IL-8 mRNA expression in esophageal mucosa of patients with non-erosive reflux disease

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World Journal of Gastroenterology 2003年第12期9卷 2801-2804页

作者： YuseiKanazawa HajimeIsomoto Chun-YangWen Ai-PingWang VladimirASaenko AkiraOhtsuru FuminaoTakeshima KatsuhisaOmagari YoheiMizuta IkuoMurata ShunichiYamashita ShigeruKohno SecondDepartmentofInternalMedicine AtomicBombDiseaseInstituteNagasakiUniversitySchoolofMedicineNagasakiJapan DepartmentofMolecularPathology AtonaicBombDiseaseInstituteNagasakiUniversitySchoolofMedicineNagasakiJapan DepartmentofMolecularMedicine AtomicBombDiseaseInstitute~NagasakiUniversitySchoolofMedicineNagasakiJapan DepartmentofPharmacotherapeutids NagasakiUniversityGraduateSchoolofBiomedicalSciencesNagasakiJapan

AIM:Little has been known about the pathogenesis of non- erosive reflux disease(NERD).Recent studies have implicated interleukin 8(IL-8)in the development and progression of gastroesophgeal reflux disease(GERD).The purpose of this study was to determine IL-8 RNA expression levels in NERD patients with or without subtle mucosal changes. METHODS:We studied 26 patients with NERD and 13 asymptomatic *** sample was taken from the esophagus 3 cm above the gastroesophageal junction and snap frozen for measurement of IL-8 mRNA levels by real-time quantitative polymerase chain reaction(PCR).We also examined mRNA expression of IL-8 receptors,CXCR-1 and -2 by reverse transcriptase *** patients were endoscopically classified into grade M(mucosal color changes without visible mucosal break)and N(neither minimal involvement nor mucosal break)of the modified Los Angeles classification. RESULTS:The relative IL-8 mRNA expression levels were significantly higher in esophageal mucosa of NERD patients than those in esophageal mucosa of the *** was a significant difference in IL-8 mRNA levels between grades M and *** CXCR-1 and -2 mRNAs were constitutively expressed in esophageal ***: Our results suggest that high IL-8 levels in esophageal mucosa may be involved in the pathogenesis of NERD through interaction with its receptors. NERD seems to be composed of a heterogeneous population in terms of not only endoscopically minimal involvement but also immune and inflammatory processes.

关键词： Adult Aged Alcohol Drinking Base Sequence Comparative Study DNA Primers Endoscopy, Digestive System Female Gastroesophageal Reflux gene expression regulation Helicobacter Infections Helicobacter pylori Hernia, Hiatal Humans Interleukin-8 Male Middle Aged RNA, Messenger Reverse Transcriptase Polymerase Chain Reaction Risk Factors Smoking

Coordinated regulation for nature products discovery and overproduction in Streptomyces

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Synthetic and Systems Biotechnology 2020年第2期5卷 49-58页

作者： Qun Zhou Shuqing Ning Yunzi Luo Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education) School of Chemical Engineering and TechnologyTianjin UniversityTianjin300072China Collaborative Innovation Center of Chemical Science and Engineering(Tianjin) Tianjin UniversityTianjin300072China

Streptomyces is an important treasure trove for natural products *** recent years,many scientists focused on the genetic modification and metabolic regulation of Streptomyces to obtain diverse bioactive compounds with high *** review summarized the commonly used regulatory strategies for natural products discovery and overproduction in Streptomyces from three main aspects,including regulator-related strategies,promoter engineering,as well as other strategies employing transposons,signal factors,or feedback *** is expected that the metabolic regulation network of Streptomyces will be elucidated more comprehensively to shed light on natural products research in the future.

关键词： gene expression regulation Natural products Streptomyces Biosynthetic gene clusters