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Cloning and Characterization of a Novel gene GmMF1 in Soybean (Glycine max L. Merr.)

Agricultural Sciences in China 2011年第12期10卷 1834-1841页

作者： JIANG Wei YANG Shou-ping YU De-yue GAI Jun-yi Soybean Research Institute Nanjing Agricultural University/National Center for Soybean Improvement/National Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing 210095 P.R. China

Cytoplasmic male sterility plays an important role in utilization of crop heterosis. Screening of soybean for novel genes related to male sterility in soybean could provide a basis for studying the molecular mechanism of male sterility in plants. In this study, gene differential expressions between the cytoplasmic male-sterile line NJCMS 1A and its maintainer line NJCMS 1B in soybean were analyzed using cDNA-AFLP. A differentially expressed fragment, GmMF-T4A15, was isolated from large flower buds of NJCMS1B. By searching the soybean genomic library and PCR amplification, the cDNA fulllength sequence of 1 311 bp was obtained and named GmMF1. The expression characteristics of GmMF1 were studied by semiquantitative real-time PCR and real-time quantitative PCR. The results showed that GmMF1 was expressed highly in flower buds of NJCMS1B. The deduced protein contains 436 amino acids and shows high similarity to members of the DUF620 protein family with unknown functions in other plant species. It is predicted that the protein encoded by GmMF1 is localized in the nucleus.

关键词： soybean cytoplasmic male sterility cDNA-AFLP gene differential expressions

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