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Characterization of Class 1 Integron Resistance gene cassettes and Salmonella Genomic Island 1 among Salmonella Strains from Healthy Humans in Northern China

Characterization of Class 1 Integron Resistance Gene Cassett...

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第七届全国医学生物化学与分子生物学和第四届全国临床应用生物化学与分子生物学联合学术研讨会暨医学生化分会会员代表大会

作者： Jian-hong Zhao Hong-bo Zhao Dong-yan Shi Ji-hong Li Shi-ying Fan Hong-li Zhao Bao-hong Xu Suju Sun Lei Shi Department of Clinical Laboratory Sciences Second HospitalHebei Medical University Center for Disease Control of Shijiazhuang College of Public Health Hebei Medical University College of Light Industry and Food Sciences South China University of Technology

Objectives:The aim of this study was to analyse the distribution and effect on the antibiotic resistance mechanisms of class 1 integrons and Salmonella genomic island 1(SGI1) in Salmonella strains obtained from healthy humans in China. Methods:Fifty-eight Salmonella strains of 25 serovars isolated from healthy humans in Shijiazhuang,northern China,in 2005 and 2007 were tested for susceptibility to 15 common

关键词： integron antibiotic resistance gene cassette Salmonella genomic island 1

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Abstract:Characterization of Class 1 Integron Resistance gene cassettes and Salmonella Genomic Island 1 among Salmonella Strains from Healthy Humans in Northern China

Abstract:Characterization of Class 1 Integron Resistance Gen...

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第二届中国临床微生物学大会暨微生物学与免疫学三江论坛

Objectives:The aim of this study was to analyse the distribution and effect on the antibiotic resistance mechanisms of class 1 integrons and Salmonella genomic island 1（SGI1） in Salmonella strains obtained from healthy humans in ***:Fifty-eight Salmonella strains of 25 serovars isolated from healthy humans in Shijiazhuang,northern China,in 2005 and 2007 were tested for susceptibility to 15 common antibiotics and the presence of class ***:Fifty-one strains（87.9%） were resistant to at least one antibiotic;the most common resistance phenotype was to streptomycin（82.8%）.Five strains were positive for the class 1 integrase gene（intll）,three in 2005 and two in *** different cassette arrays were detected:aadA2,blaPSE-1,and *** conjugation indicated that all the detected integrons were on plasmids and could transfer to other *** showed that there was no direct correlation between the integron-positive and integron-negative ***1 was detected in a *** strain,with the aadA2,floR,tetG,and blaPSE-1 genes in its multidrug resistance region,and it was located between thdF and a retronphage（int2）.Conclusions:To our knowledge,this is the first report on SGIl in strains from healthy *** presence of class 1 integrons and SGIl in Salmonella strains from healthy humans suggests that widespread surveillance of antibiotic resistance should be conducted at the gene level.

关键词： integron antibiotic resistance gene cassette Salmonella genomic island 1

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Molecular characterization of common promoters and gene cassettes of class 1 integrons in uropathogenic Enterobacteriaceae strains

Molecular characterization of common promoters and gene cass...

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2011年浙江省检验医学学术年会

作者：魏取好胡庆丰吕火祥张萍周永列浙江省人民医院检验中心

Objectives To investigate the molecular characterization of common promoters as well as their associated gene cassettes of class 1 integrons in uropathogenic Enterobacteriaceae strains. Methods 227 Enterobacteriaceae strains were isolated from urines of *** specific for class 1 integron integrase gene intll were used in PCR assay for screening class 1 *** variable regions of class 1 integrons were amplified and identified by using restriction analysis with Hinf I and Cfrl3 I combined with sequence *** common promoters upstream of each gene cassettes arrays were then identified by sequences analysis. Results Class 1 integron were detected in 157(69%,157/227) *** one different gene cassettes and eighteen different gene cassette arrays were detected in this *** most prevalent common promoter was PcHl,a relative weak promoter was detected in 90 of 173 class 1 integrons. PcWTGN-10,PcS,PcW and PcH2TGN-10 were detected in 43,19,12 and 7 class 1 integrons respectively. Certain gene cassettes array or gene cassette was mainly associated with the same common promoter that detected in different isolates. Conclusions The most gene cassettes harboured in class 1 integron in uropathogenic Enterobacteriaceae strains were those that confered resistance to traditional *** same gene cassettes arrays were detected in different genus,the upstreamed promoter of certain gene cassettes arrays were remained stability,indicated the spread of the whole class 1 integron among different *** than half of these integrons detected in this study harboured relatively weak common promoters,indicating these integrons have strong capability of capture exogenous gene cassettes.

关键词： integron gene cassette promoter antibiotic resistance mobile genetic elements

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Detection of class 1 integron in Acinetobacter baumannii isolates collected from nine hospitals in Turkey

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Asian Pacific Journal of Tropical Biomedicine 2013年第9期3卷 743-747页

作者： Aysegül Copur Cicek Azer Ozad Düzgün Aysegül Saral Tuba Kayman Zeynep Cizmeci Pervin Ozlem Balci Tuba Dal Mehmet Firat Ismail Tosun Yasemin Ay Alitntop Ahmet Caliskan Yelda Yazici Cemal Sandalli Department of Medical Microbiology Faculty of MedicineRecep Tayyip Erdogan University Department of genetics and Bioengineering Faculty of Engineering and Natural SciencesGümüshane University Department of Biology Faculty of Arts and SciencesArtvin Coruh University Clinical Microbiology Laboratory Research and Training Hospital Clinical Microbiology Laboratory Kecioren Research and Training Hospital Clinical Microbiology Laboratory General State Hospital Department of Medical Microbiology Faculty of MedicineDicle University Clinic of Infectious Diseases and Clinical Microbiology PrivateOSM Ortadogu Hospital Microbiology Laboratory Kirikkale Highly Specialized Hospital general State Hospital Microbiology Laboratory Necip Fazil City Hospital Clinical Microbiology Laboratory Ahi Evren Chest and Cardiovascular Surgery Research and Training Hospital Department of Biology Faculty of Arts and SciencesRecep Tayyip Erdogan University

Objective:To investigate the antibiotic resistance genes inserted into class I and class 2integrons in Acinetobacler baumannii[***)isolates obtained from nine different cities in ***:A collection of 281 *** clinical isolates were collected from nine diferenl state hospitals in Turkey and were confirmed as *** by conventional biochemical,API testing and bla_(-OXA-51)specific *** isolates were examined by PCR for existence of class I and2 integron gene ***:They were characterized by antimicrobial susceptibility testing and the highest resistance rates were determined for piperacillin(90.03%),ciprofloxacin(87.54%),cefepime and trimethoprim/sulfamethoxazole(81.13%).The lowest resistance rates was for cefotaxime(3.55%).class 1 integrons were detected in 6.4%(18/281)of *** strains and no class 2 integron was *** gene casselles of class 1 inlegrons AacCI-AAC(3)l-aadAI,AacCI-aadA1,AAC(3)-I,AAC(3)-I-AAC(3)-I-aadA1,***(3)-I-aadA1-AAC(3)-I-AAC(3)-I,AAC(3)-I-AAC(3)-I-AAC(3)-I-aadA1,AAC(3)-I-aadA1,AAC(3)-I-AAC(3)-I,AAC(3)-I-aadA1-AAC(3)-I-aadA1,AAC(3)-I-AAC(3)-I-aadA1-AAC(3)-I-aadA1 were detected in eighteen *** aac genes family were most frequently found integrated into the class 1 inlegrons and it was followed by aadA genes and TEM-1 ***:This is an extensive study on the distribulion of class 1 integron among *** in *** addition to these,two new alleles were *** percentage rates of similarity to other cassettes are 95%aadA1(TK A18)and 89%,aadA 1(ANKA3).

关键词： Acinetobacter baumannii Class 1 integron gene cassette Resistance

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Prevalence of Carbapenem-Resistant Klebsiella Pneumoniae （CRKP） and the Distribution of Class 1 Integron in Their Strains Isolated from a Hospital in Central China

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Chinese Medical Sciences Journal 2017年第2期32卷 107-112页

作者： QinOu WenfangLi BeiLi ChunfangYu Departmentof Medical Microbiology Laboratory of Infectious Diseases Taihe Hospital Hubei University of Medicine Shiyan Hubei 442000 China

Objective The aim of our study is to investigate the prevalence of Carbapenem-resistant Klebsiella pneumoniae （CRKP） and the genetic characteristics of the class 1 integron in CRKP on multi-drug resistance. Methods Clinical Klebsiella pneumoniae strains were collected from multiple departments of a hospital in central China. CRKP strains were identified among the isolates, and antibiotics susceptibility of CRKP strains was analyzed. The polymerase chain reaction （PCR） was adopted to amplify the class 1 integron variable area. The integron genetic structure was analyzed with enzyme digestion and DNA sequencing technology. The relation between class 1 integron and drug resistance was analyzed statistically. Results Totally 955 strains of Klebsiella pneumoniae were isolated from varied sites of the hospital, and 117（12.3%） of them were identified as CRKP, with a separation rate of 8.9% （26/292） in 2013, 11.3% （38/336） in 2014 and 16.2% （53/327） in 2015, which shows an increasing trend by year. 44.4% （52/117） of CRKP strains were separated from specimen of ICU, and 61.5% （72/117） were from sputum. Over 95% CRKP strains were resistant to ampicillin/sulbactam, aztreonam, imipenem, meropenem Ceftazidme,Cefotaxime,Cefepime,and Piperacillin, while relatively low resistant rates were found in Tigecycline （12.8%） and colistin （35.9%）. The class 1 integron was detected in 77.8% （91/117） of CRKP strains. Class 1 integron of CRKP was significantly correlated with the antibiotic resistance to the tobramycin, gentamicin and amikacin （all P〈0.01）. The gene cassette analysis of variable area of class 1 integron showed that aadA2 accounts for 64.8% （59/91）, aacA4-catB8-aadA1 23.1% （21/91）, and aadA2-dfrA25 12.1% （11/91）. Conclusions CRKP has an increasing trend in a clinical setting in China, and most of them were resistant to multiple antibiotics. Class 1 integron in CRKP has strong ability to capture the genes resistant to aminoglycosides antibiotics from environment, with th

关键词： carbapenem resistance klebsiella pneumoniae integron gene cassette

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Class Ⅰ integron with a novel cassette array in an ESBL-producing multidrug-resistant Klebsiella pneumoniae isolate

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Journal of Nanjing Medical University 2006年第1期20卷 12-16页

作者： Bing Gu Mingqing Tong Wangsheng Zhao Shiyang Pan Yuanhua Wei Peijun Huang Department of Clinical Laboratories the First Affiliated Hospital of Nanjing Medical University Nanjing 210029 China

Objective： To analyze the molecular mechanism of integron mediated mulfi-resistanc, e in an ESBL-producing K. pneumoniae NJ 12 isolate. Methods： Susceptibility test was carried out by Kirby-Bauer method. Class Ⅰ, Ⅱ and Ⅲ integrons were detected by integrase gene PCR with primers that annealed to conserved regions of integron-encoded integrase genes intll, intl2 and intl3. The variable region of integron was amplified by integron PCR with primers that targeted the conserved flanking regions, and the product was sequenced. Six aminoglycoside modifying-enzyme genes, including ant（2＂）-Ⅰ, ant（3＂）- Ⅰ, aac（3）- Ⅰ, aac（3）-Ⅱ, aac （6＇）-Ⅰ, and aac（6＇）-Ⅱ , were detected. Results： K. pneumoniae NJ 12 was resistant to nine antibiotics, including piperacillin, ampicillin, cefuroxime, ceftazidime, cefotaxime, aztreonam, streptomycin, gentamicin and amikacin. This isolate was shown that there was positive with class Ⅰ integron, ant（2＂）- Ⅰ , ant（3＂）- Ⅰ , aac（3）-Ⅱ and aac（6＇）- Ⅰ modifying-enzyme genes. Neither class Ⅱ nor Ⅲ integron was detected; DNA sequencing of the fragment amplified by integron PCR revealed a novel cassette array aadR-cat-blaoxa-10/ aadA1. Conclusion： Class I integron with a novel cassette array in an ESBL-producing multidrug-resistant K. pneumon/ae NJ 12 isolate was reported from Nanjing area of China, with the GenBank accession number DQ141319.

关键词： integron gene cassette multi-resistance K.pneumoniae ESBL

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Survey of Multidrug Resistance and Class I Integron Prevalence in Chicken-derived Salmonella

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Agricultural Science & Technology 2014年第5期15卷 877-881页

作者：陆彦赵红玉张中文候晓林吴国娟北京农学院北京102206 北京市生命科学研究所北京102206

[Objective] This study aimed to investigate the multidrug resistance and prevalence of class I integrons in Salmonel a. [Method] Salmonel a strains were isolated from chicken farms in Shandong Province. Kauffmann-White classification method was employed to analyze the serotypes of Salmonel a strains. Minimum in-hibition concentration （MIC） of Salmonel a strains against 19 common antimicrobial drugs was analyzed determined with microdilution method. The class I integrons and carried drug resistance gene cassettes were detected by PCR. [Result] A total of 311 Salmonel a strains were isolated and classified into two serotypes, including 133 Salmonel a Indiana strains and 178 Salmonel a Enteritidis strains. Drug sensitivity test showed that the isolated Salmonel a strains were general y resistant to sulfadiazine, sulfamethoxazole, nalidixic acid, ampicil in, tetracycline, doxycycline and trimethoprim, with a multidrug resistance rate of 91.0% （283/311）; 99% strains were sensitive to amikacin and colistin. PCR assay indicated that the detection rate of class I integrons was 65.0% （202/311）; the positive rate of class I integrons in Salmonel a strains with multidrug resistance was 92.6%; among 202 positive strains, six strains carried gene cassette dfr17-aadA5. [Conclusion] According to the above results, class I integrons exist general y in Salmonel a and are closely associated with the multidrug resistance of Salmonel a strains.

关键词： Salmonella Multidrug resistance Class I integron gene cassette

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