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Egg cell-secreted aspartic proteases ECS1/2 promote gamete attachment to prioritize the fertilization of egg cells over central cells in Arabidopsis

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Journal of Integrative Plant Biology 2022年第11期64卷 2047-2059页

作者： Jiahao Jiang Nils Stührwohldt Tianxu Liu Qingpei Huang Ling Li Li Zhang Hongya Gu Liumin Fan Sheng Zhong Andreas Schaller Li-Jia Qu State Key Laboratory for Protein and Plant Gene Research Peking-Tsinghua Center for Life Sciences at College of Life SciencesPeking UniversityBeijing 100871China Department of Plant Physiology and Biochemistry Institute of BiologyUniversity of HohenheimStuttgart 70593Germany

Double fertilization is an innovative phenomenon in angiosperms,in which one sperm cell first fuses with the egg cell to produce the embryo,and then the other sperm fuses with the central cell to produce the ***,the molecular mechanism of the preferential fertilization of egg cells is poorly *** this study,we report that two egg cell-secreted aspartic proteases,ECS1 and ECS2,play an important role in promoting preferential fertilization of egg cells in *** show that simultaneous loss of ECS1 and ECS2 function resulted in an approximately 20%reduction in fertility,which can be complemented by the full-length ECS1/2 but not by corresponding active site mutants or by secretion-defective versions of ECS1/*** phenotypic analysis revealed that the egg cellsperm cell attachment was compromised in ecs1 ecs2 *** pollination assays with cyclin-dependent kinase a1(cdka;1)pollen showed that preferential egg cell fertilization was impaired in the ecs1 ecs2 *** together,these results demonstrate that egg cells secret two aspartic proteases,ECS1 and ECS2,to facilitate the attachment of sperm cells to egg cells so that preferential fertilization of egg cells is *** study reveals the molecular mechanism of preferential fertilization in Arabidopsis thaliana.

关键词： Arabidopsis thaliana double fertilization ECS1/2 gamete interaction preferential fertilization

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ERp57 is a potential biomarker for human fertilization capability

ERp57 is a potential biomarker for human fertilization capab...

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第一届中华医学会生殖医学分会、中国动物学会生殖生物学分会联合年会

作者： Jingjing Zhang Jindao Wu Ran Huo Yingzi Mao Ying Lu Xuejiang Guo Jiayin Liu Zuomin Zhou Xiaoyan Huang~* Jiahao Sha Laboratory of Reproductive Medicine,Department of Histology and Embryology,Nanjing Medical University,Nanjing,210029,China

Human infertility is a growing concern and while many assisted reproductive technologies exist their success rates are ***,developing tests,possibly by assessing proteins involved in fertilization that could predict the outcome of these technologies is of great *** identify candidate proteins,we used two-dimensional PAGE and MALDI-TOF techniques and detected the ERp57 protein from human testis protein profile,Immunohistochemistry showed that ERp57 was mostly located on spermatogenic cell cytoplasm from spermatocyte to the spermatozoa phases and Leydig cells of human testes;it was present in low levels in Sertoli ***,ERp57 was evident in human spermatozoa,primarily in the acrosome and tail,and was phosphorylated in association with human *** ERp57 with antibodies significantly inhibited human sperm from penetrating zona- free hamster eggs in a dose-dependent ***,expression levels of ERp57 were associated with fertility;they were decreased dramatically in IVF patients with low fertilization rate compared to those with a high rate or fertile sperm *** together,these results show that ERp57 is a component of human sperm acrosome proteins that play a critical role in gamete ***,ERp57 could be a novel phenotype marker for male infertility and has the potential to be used to assess sperm selections for IVF.

关键词： ERp57 testes capacitaiton gamete interaction

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