检索结果-南通市图书馆

Cryo-EM structure of glycoprotein C from Crimean-Congo hemorrhagic fever virus

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Virologica Sinica 2022年第1期37卷 127-137页

作者： Na Li Guibo Rao Zhiqiang Li Jiayi Yin Tingting Chong Kexing Tian Yan Fu Sheng Cao CAS Key Laboratory of Special Pathogens Center for Biosafety Mega-ScienceWuhan Institute of VirologyChinese Academy of SciencesWuhan430071China University of Chinese Academy of Sciences Beijing100049China

Crimean-Congo hemorrhagic fever virus(CCHFV)is a causative agent of serious hemorrhagic diseases in humans with high mortality *** glycoprotein Gc plays critical roles in mediating virus-host membrane fusion and has been studied extensively as an ***,the molecular mechanisms involved in membrane fusion and Gc-specific antibody-antigen interactions remain unresolved largely because structural information of this glycoprotein is *** designed a trimeric protein including most of the ectodomain region of Gc from the prototype CCHFV strain,Ib Ar10200,which enabled the cryo-electron microscopy structure to be solved at a resolution of 2.8Å.The structure confirms that CCHFV Gc is a class Ⅱ fusion ***,structural comparisons with other solved Gc trimers in the postfusion conformation revealed that CCHFV Gc adopted hybrid architectural features of the fusion loops from hantaviruses and domain Ⅲ from phenuiviruses,suggesting a complex evolutionary pathway among these *** sites on CCHFV Gc that protective neutralizing antibodies target were mapped onto the CCHFV Gc structure,providing valuable information that improved our understanding of potential neutralization mechanisms of various antibodies.

关键词： Crimean-Congo hemorrhagic Fever virus(CCHFV) Glycoprotein C fusion protein Bunyavirus

Improved pinene production in a recombinant yeast by fusion linker optimization and chaperon coexpression

在线全文

学校读者我要写书评

暂无评论

Systems Microbiology and Biomanufacturing 2022年第1期2卷 208-216页

作者： Quanlu Ren Yaxi He Xinyao Lu Hong Zong Bin Zhuge The Key Laboratory of Industrial Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi 214122China Research Center of Industrial Microbiology School of BiotechnologyJiangnan UniversityWuxi 214122China

Pinene is an active natural monoterpene from plants and has important applications in favorings,fragrances,and ***,pinene dimers are regarded as renewable fuels with high ***,the microbial pinene production was limited by the low activity pinene *** this study,the pinene synthase activity was improved by fusion linker optimization and chaperon *** construct the pinene pathway in Saccharomyces cerevisiae,YPL062W gene was deleted to increase the MVA pathway precursor *** 3-hydroxyl-3-methylglutaryl-CoA reductase(tHMG1),isopentenyl-diphosphate isomerase(IDI1),and farnesyl diphosphate synthase mutant(ERG20F96W−N127W)were then integrated to improve the GPP *** synthase tPt1 was expressed in the constructed engineered yeast,and the titer of pinene reached 0.166 mg/*** is the direct precursor of pinene,ERG20ww and tPt1 were fused by diferent linkers and orders to improve the accessibility of *** titer reached 9.94 mg/L by fusion these proteins in the order of ERG20ww and tPt1 and with a fexible linker(G)*** that,several chaperons were coexpressed and the chaperon Sil1p improved the pinene titer to 10.2 mg/L with a yield of 1.63 mg/L·*** results presented here provide novel information on the applications of protein fusion and protein chaperons in microbial pinene production.

关键词： Pinene Saccharomyces cerevisiae fusion protein Oligopeptide linker Chaperones

人白细胞介素－2－绿脓杆菌外毒素融合蛋白的初步研究

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 1995年第4期27卷 453-458页

作者：高基民黄洪莲郑仲承徐舲飞刘新垣中国科学院上海生物化学研究所第一军医大学免疫学教研室

白细胞介素－２：绿脓杆菌外毒素；融合蛋白；原核表达；细胞毒活性

关键词： Interleukin-2 Pseudomonas exotoxin fusion protein Prokaryotic expression Cytotoxic activity

Albumin fusion with granulocyte-macrophage colony-stimulating factor acts as an immunotherapy against chronic tuberculosis

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2021年第10期18卷 2393-2401页

作者： Yu-Min Chuang Liangmei He Michael L.Pinn Ya-Chea Tsai Max A.Cheng Emily Farmer Petros C.Karakousis Chien-Fu Hung Department of Pathology Johns Hopkins University School of MedicineBaltimoreMDUSA Department of Medicine Johns Hopkins University School of MedicineBaltimoreMDUSA Section of Infectious Diseases Department of Internal MedicineYale University School of MedicineNew HavenCTUSA

A long duration of treatment and emerging drug resistance pose significant challenges for global tuberculosis(TB)eradication ***,there is an urgent need to develop novel strategies to shorten TB treatment regimens and to treat drug-resistant *** an albumin-fusion strategy,we created a novel albumin-fused granulocyte-macrophage colony-stimulating factor(albGM-CSF)molecule that harnesses albumin’s long half-life and targeting abilities to enhance the biostability of GM-CSF and direct it to the lymph nodes,where the effects of GM-CSF can increase dendritic cell populations crucial for eliciting a potent immune *** this study,we demonstrate that albGM-CSF serves as a novel immunotherapy for chronic Mycobacterium tuberculosis(Mtb)infections by enhancing GM-CSF biostability in ***,albumin is very safe,stable,and has a long half-life,thereby enhancing the biostability of *** the lungs and draining lymph nodes,albGM-CSF is able to increase the numbers of dendritic cells,which are crucial for the activation of naive T cells and for eliciting potent immune *** administration of albGM-CSF alone reduced the mean lung bacillary burden in mice with chronic tuberculosis *** GM-CSF administration was associated with IL-1βrelease from Mtb-infected dendritic cells and macrophages,higher IL-1βlevels were observed in albGM-CSF-treated mice with chronic tuberculosis infection than in mice receiving *** fusion with GM-CSF represents a promising strategy for the control of chronic lung tuberculosis infections and serves as a novel therapeutic vaccination platform for other infectious diseases and malignancies.

关键词： Tuberculosis albumin GM-CSF therapeutic vaccine fusion protein

Molecular Cloning and Bacterial Expression of Germacrene A Synthase cDNA from Crepidiastrum sonchifolium

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2006年第5期22卷 606-611页

作者： REN Jie LIU Yan-qiu YANG Li NI Rui YOU Song School of Pharmaceutical Engineering Shenyang Pharmaceutical UniversityShenyang 110016 P. R. China

Germacrene A synthase（GAS） catalyzes the biosynthesis of germacrene A, which is a key precursor for sesquiterpone lactones. Cloning of a novel full-length cDNA encoding GAS from the medicinal plant Crepidiastrum sonchifolium（dosignated CsGAS） is reported in this study. The cDNA is 1837 bp long and contains a 1680-bp open reading frame encoding a 559 amino-acid protein. The functional expression of the cDNA in Escherichia coli, as an N-terminal thioredoxin fusion protein, with the pET32a vector yielding a recombinant enzyme. Sequence analysis was used to compare this enzyme with the mechanistically related epi-aristolochene synthase from tobacco, and the effect of possible involvement of a number of amino acids in sesquiterpone synthase on product specificity was also discussed.

关键词： Germacrene A synthase Crepidiastrum sonchifolium Sesquiterpene synthase Expression fusion protein

Saccharomyces cerevisiae in neuroscience:how unicellular organism helps to better understand prion protein?

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2021年第3期16卷 489-495页

作者： Takao Ishikawa Department of Molecular Biology Institute of BiochemistryFaculty of BiologyUniversity of WarsawWarsawPoland

The baker's yeast Saccharomyces(S.)cerevisiae is a single-celled eukaryotic model organism widely used in research on life *** a unicellular organism,*** has some evident limitations in application to ***,yeast prions are extensively studied and they are known to share some hallmarks with mammalian prion protein or other amyloidogenic proteins found in the pathogenesis of Alzheimer's,Parkinson's,or Huntington's ***,the yeast *** has been widely used for basic research on aggregation properties of proteins in cellulo and on their ***,a yeast-based study revealed that some regions of mammalian prion protein and amyloidβ1–42 are capable of induction and propagation of yeast *** is one of the examples showing that evolutionarily distant organisms share common mechanisms underlying the structural conversion of prion proteins making yeast cells a useful system for studying mammalian prion *** has also been used to design novel screening systems for anti-prion compounds from chemical *** assays are cheap in maintenance and safe for the researcher,making them a very good choice to perform preliminary screening before further characterization in systems engaging mammalian cells infected with *** this review,not only classical red/white colony assay but also yeast-based screening assays developed during last year are *** analysis and research carried out using yeast prions force us to expect that prions are widely present in ***,the last few years brought us several examples indicating that the mammalian prion protein is no more peculiar protein–it seems that a better understanding of prion proteins nature-wide may aid us with the treatment of prion diseases and other amyloid-related medical conditions.

关键词： amyloid artificial prion baker's yeast budding yeast drug screening fusion protein neurodegenerative diseases prion protein yeast-based assay

Cox7a2 mediates steroidogenesis in TM3 mouse Leydig cells

在线全文

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2006年第5期8卷 589-594页

作者： Liang Chen Zhong-Cheng Xin Xin Li Long Tian Yi-Ming Yuan Gang Liu Xue-Jun Jiang Ying-Lu Guo Andrology Center of Peking University First Hospital Beijing 100009 China Department of Surgery People＇s Hospital of Zhangqiu Zhangqiu 250200 China Institute of Microbiology Chinese Academy of Sciences Beijing 100080 China

Aim： To investigate the regulatory function of Cox7a2 on steroidogenesis and the mechanism involved in TM3 mouse Leydig cells. Methods： The cDNA of Cox7a2 was cloned from TM3 mouse Leydig cells. It was subcloned to pDsRed- Express-N 1 and transfected back into TM3 mouse Leydig cells for Cox7a2 overexpression by transient gene transfection. Steroidogenesis affected by overexpressed Cox7a2 was studied by ELISA. To elicit the mechanism of this effect, expression of steroidogenic acute regulatory （STAR） protein and reactive oxygen species （ROS） were examined by Western blot and fluorometer, respectively. Results： The cDNA of Cox7a2 （249 bp） was cloned from Leydig cells and confirmed by DNA sequencing. After constructed pDsRed-Express-N1-Cox7a2 was transfected back into TM3 mouse Leydig cells, Cox7a2 inhibited not only luteinizing hormone （LH）-induced secretion of testosterone but also the expression of StAR protein. At the same time, Cox7a2 increased the activity of ROS in TM3 mouse Leydig cells. Conclusion： Cox7a2 inhibited LH-induced StAR protein expression, and consequent testosterone production, at least in part, by increasing ROS activity in TM3 mouse Leydig cells.

关键词： Cox7a2 fusion protein steroidogenesis steroidogenic acute regulatory protein reactive oxygen species Leydig cell

Construction of a eukaryotic expression plasmid for human retina-derived neurotrophin-3

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2013年第11期8卷 1031-1040页

作者： Chunxia Peng Xiaobei Yin Mengda Li Ting He Genlin Li Beijing Tongren Eye Center Beijing Tongren Hospital Capital Medical University Beijing Key Laboratory of Ophthalmology & Visual Sciences

Neurotrophin-3 （NT-3） can promote the repair of central nervous system and retinal damage. In previous reports, NT-3 has been expressed by viral vectors. However, plasmid vectors have a safer profile compared with viral vectors in clinical studies. This study recombined amplified human retinal NT-3 with a eukaryotic expression plasmid containing green fluorescent protein （GFP） to construct an NT-3 expression plasmid, pEGFP-N1-NT-3. The transfection efficiency 48 hours after pEGFP-N1-NT-3 transfection to 293T cells was 50.06 ＋ 2.78%. Abundant NTo3-GFP was expressed in 293T cells as observed by fluorescence microscopy, suggesting the construct pEGFP-N1-NT-3 effectively expressed and secreted NT-3-GFP. Secretory vesicles containing NT-3-GFP were observed in a constant location in cells by laser scan confocal microscopy, indicating the expression and secretion processes of NT-3 in eukaryotic cells were in accordance with the physical synthesis processes of secreted proteins. Western blot assay showed that pro-NTo3-GFP had a molecular weight of 56 kDa, further confirming NT-3-GFP expression. At 48 hours after transfection, the concentration of NT-3 in culture medium was 22.3 ng/mL, suggesting NT-3 produced by pEGFP-N1-NT-3 was efficiently secreted. This study constructed a human retinal-derived NT-3 eukaryotic expression plasmid that efficiently expressed and secreted NT-3.

关键词： neural regeneration gene therapy biological factor neurotrophin-3 plasmid fusion protein encapsulated cell technology retinitis pigmentosa grants-supported paper neuroregeneration

Human fused NKG2D-IL-15 protein controls xenografted human gastric cancer through the recruitment and activation of NK cells

在线全文

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2017年第3期14卷 293-307页

作者： Yan Chen Bei Chen Ti Yang Weiming Xiao Li Qian Yanbing Ding Mingchun Ji Xiaoqun Ge Weijuan Gong Department of Immunology School of Medicine Yangzhou University Yangzhou 225001 People's Republic of China Department of Pharmacology School of Medicine Yangzhou University Yangzhou 225001 People's Republic of China Department of Gastroenterology The Second Affiliated Hospital Yangzhou University Yangzhou 225001 People's Republic of China Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases Yangzhou University Yangzhou 225009 People's Republic of China Jiangsu Key Laboratory of Zoonosis Yangzhou University Yangzhou 225009 People's Republic of China Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou 225009 People's Republic of China

Interleukin （IL）-15 plays an important role in natural killer （NK） and CD8＋ T-cell proliferation and function and is more effective than IL-2 for tumor immunotherapy. The trans-presentation of IL-15 by neighboring cells is more effective for NK cell activation than its soluble IL-15. In this study, the fusion protein dsNKG2D-IL-15, which consisted of two identical extracellular domains of human NKG2D coupled to human IL-15 via a linker, was engineered in Escherichia coll. DsNKG2D-IL- 15 could efficiently bind to major histocompatibility complex class I chain-related protein A （MICA） of human tumor cells with the two NKG2D domains and trans-present IL-15 to NK or CD8＋ T cells. We transplanted human gastric cancer （SGC-7901） cells into nude mice and mouse melanoma cells with ectopic expression of MICA （B 16BL6-MICA） into C57BL/6 mice. Then, we studied the anti-tumor effects mediated by dsNKG2D-IL-15 in the two xenografted tumor models. Human dsNKG2D-IL-15 exhibited higher efficiency than IL-15 in suppressing gastric cancer growth. Exogenous human dsNKG2D-IL-15 was centrally distributed in the mouse tumor tissues based on in vivo live imaging. The frequencies of human CD56＋ cells infiltrated into the tumor tissues following the injection of peripheral blood mononuclear cells into nude mice bearing human gastric cancer were significantly increased by human dsNKG2D-I L- 15 treatment. Human dsNKG2 D-I L- 15 also delayed the growth of transplanted melanoma （B 16BL6- MICA） by activating and recruiting mouse NK and CD8＋ T cells. The anti-melanoma effect of human dsNKG2D-IL-15 in C57BL/6 mice was mostly decreased by the in vivo depletion of mouse NK cells. These data highlight the potential use of human dsNKG2D-IL-15 for tumor therapy.

关键词： fusion protein IL-15 NK cells NKG2D tumor