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Synergistic regulation of fusion pore opening and dilation by SNARE and synaptotagmin-1

Journal of Molecular Cell Biology 2024年第4期16卷 17-30页

作者： Kaiju Li Kaiyu Li Jiaqi Fan Xing Zhang Chengyan Tao Yijuan Xiang Lele Cui Hao Li Minghan Li Yanjing Zhang Jia Geng Ying Lai National Clinical Research Center for Geriatrics State Key Laboratory of Biotherapy and Collaborative Innovation Center of BiotherapyWest China HospitalSichuan UniversityChengdu 610041China Department of Laboratory Medicine State Key Laboratory of Biotherapy and Cancer CenterWest China HospitalSichuan University and Collaborative Innovation CenterChengdu 610041China Tianfu Jincheng Laboratory City of Future MedicineChengdu 641400China

fusion pore opening is a transient intermediate state of synaptic vesicle exocytosis,which is highly dynamic and precisely regulated by the soluble N-ethylmaleimide-sensitive factor attachment protein receptor(SNARE)complex and synaptotagmin-1(Syt1).Yet,the regulatory mechanism is not fully *** this work,using single-channel membrane fusion electrophysiology,we determined that SNAREpins are important for driving fusion pore opening and dilation but incapable of regulating the *** Syt1 was added,the closing frequency of fusion pores significantly increased,while the radius of fusion pores mildly *** response to Ca^(2+),SNARE/Syt1 greatly increased the radius of fusion pores and reduced their closing ***,the residue F349 in the C2B domain of Syt1,which mediates Syt1 oligomerization,was required for clamping fusion pore opening in the absence of Ca^(2+),probably by extending the distance between the two ***,in Ca^(2+)-triggered fusion,the primary interface between SNARE and Syt1 plays a critical role in stabilizing and dilating the fusion pore,while the polybasic region of Syt1 C2B domain has a mild effect on increasing the radius of the fusion *** summary,our results suggest that Syt1,SNARE,and the anionic membrane synergically orchestrate the dynamics of fusion pore opening in synaptic vesicle exocytosis.

关键词： SNARE synaptotagmin-1 fusion pore vesicle exocytosis

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fusion pore kinetics modulated by mechanisms other than Ca2+ in adrenal chromaffin cells

Fusion pore kinetics modulated by mechanisms other than Ca2+...

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第十次中国生物物理学术大会

作者： Ning Guo, Tao Liu, Xiao-Ke Chen, Yang Zhou and Zhuan Zhou Institute of Molecular Medicine, Peking University, Beijing 100871, China.

＜正＞Exocytosis proceeds by either full fusion or ’kiss-and-run’ between vesicle and plasma membrane. Switching between these two modes permits the cell to regulate the kinetics and amount of secretion. Here we show that ATP receptor activation reduces secretion downstream from cytosolic Ca2+ elevation in rat adrenal chromaffin cells. This reduction is

关键词： chromaffin cell fusion pore secretion

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Enhanced sampling for lipid-protein interactions during membrane dynamics

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BIOCELL 2023年第1期47卷 1-14页

作者： DIEGO MASONE Instituto de Histología y Embriología de Mendoza(IHEM)-Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET) Universidad Nacional de Cuyo(UNCuyo)Mendoza5500Argentina Facultad de Ingeniería Universidad Nacional de Cuyo(UNCuyo)Mendoza5500Argentina

The inflexible concept of membrane curvature as an independent property of lipid structures is today *** bilayers behave as many-body entities with emergent properties that depend on their interactions with the *** particular,proteins exert crucial actions on lipid molecules that ultimately condition the collective properties of the *** this review,the potential of enhanced molecular dynamics to address cell-biology problems is *** cases of membrane deformation,membrane fusion,and the fusion pore are analyzed from the perspective of the dimensionality reduction by collective *** lipid-protein interactions as fundamental determinants of large membrane remodeling events are also ***,novel strategies merging cell biology and physics are considered as future lines of research.

关键词： Membrane bending Membrane fusion fusion stalk Hemifusion diaphragm fusion pore Molecular dynamics Collective variables Collective behavior Emergent properties

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Self-assembly of syntaxin-1A in model membrane explored by multiscale molecular dynamics simulation

Self-assembly of syntaxin-1A in model membrane explored by m...

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中国生物化学与分子生物学会2016年全国学术会议

作者： Jing Han Rainer A. B?ckmann Jie Ma Department of Biochemistry and Molecular Biology School of Basic Medical Sciences Xi'an Jiaotong University Health Science Center Xi’an Jiaotong University Health Science Center

Synaptic vesicle fusion in neuronal transmission requires the assembly of soluble N-ethylmaleimide-sensitive factor attachment protein receptor(SNARE)complex formed by a conserved protein family:v-SNARE,synaptobrevin ... 详细信息

关键词： Syntaxin-1A Oligomerization Self-assembly Molecular dynamics simulation Synaptic vesicle fusion fusion pore

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