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The biotechnological potential of using mono-and co-cultures of Aspergillus niger van Tieghem and Trichoderma viride Pers ex *** enhance the protein content of cassava(Manihot esculenta Crantz)peels by solid substrate fermentation

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Studies in Fungi 2019年第1期4卷 111-122页

作者： Yafetto L Odamtten GT Adu SK Ekloh E Department of Molecular Biology and biotechnology School of Biological SciencesCollege of Agriculture and Natural SciencesUniversity of Cape CoastCape CoastGhana Department of Plant and Environmental Biology School of Biological SciencesCollege of Basic and Applied SciencesUniversity of GhanaLegon-AccraGhana Department of Animal Science School of Agricultural SciencesCollege of Agriculture and Natural SciencesUniversity of Cape CoastCape CoastGhana

The application of fungal biotechnology for nutrient value addition and improvement of agro-industrial wastes is critical in the development of protein-rich feed for *** study evaluated conventional methods for protein enrichment of cassava peels by solid substrate fermentation using mono-and co-cultures of Aspergillus niger and Trichoderma *** and non-sterile cassava peels inoculated with mono-cultures of *** or *** and co-cultures of *** and *** were spontaneously fermented for 21 days at 24℃.Fermented substrates were harvested at 0,7,14,and 21 days intervals,then oven-dried at 60℃,milled in a blender and further assayed for ash,carbohydrate,crude fibre,fat,and protein *** showed that percentage increase in protein contents after 21 days incubation of sterile cassava peels were 71.02%(for mono-culture of ***)and 71.64%(for mono-culture of ***);co-culture of *** and *** yielded a 129.00%increase in *** percentage increase in protein content of the non-sterile cassava peels was 126.80%(for mono-culture of ***)and 168.34%(for mono-culture of ***)in contrast with 63.47%obtained for the co-culture of *** and *** in non-sterile cassava peels,which was attributable to possible antibiosis due to microbial *** content significantly(p≤0.05)increased for 21 days while carbohydrate,crude fibre and fat contents in both sterile and non-sterile cassava peels decreased over the same *** findings underscore the fact that protein content of cassava peels can be significantly(p≤0.05)enriched through fungal biotechnology to produce value-added feed supplement for *** practical implications of these findings are discussed.

关键词： agro-industrial wastes environmental mycoremediation fungal biotechnology nutrient-enriched feed

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Biotechnological potential of agro-industrial wastes for protein enrichment by solid-state fermentation using Aspergillus niger

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Studies in Fungi 2018年第1期3卷 176-186页

作者： Adu SK Yafetto L Ofosuhene A Offei-Affedzie T Adu S Ntibe TN Department of Molecular Biology and biotechnology School of Biological SciencesCollege of Agriculture and Natural SciencesUniversity of Cape CoastCape CoastGhana Department of Animal Science School of AgricultureCollege of Agriculture and Natural SciencesUniversity of Cape CoastCape CoastGhana

This study is to assess the biotechnological potential of agro-industrial wastes of pineapple(Ananas comosus),sweet potato(Ipomoea batatas)and watermelon(Citrullus lanatus)for protein enrichment by solid-state fermentation using Aspergillus *** suspensions of *** were prepared with potato dextrose broth that had been supplemented with sodium chloride,ammonium nitrate and thiamine,and adjusted to pH of *** protein contents of sterile and non-sterile wastes of the pineapple,sweet potato,and watermelon were determined by the Kjeldahl *** remaining sterile and non-sterile wastes was inoculated with spore suspensions of *** and incubated at temperature of 24℃ under conditions of solid-state fermentation for 14 *** contents of the inoculated sterile and non-sterile wastes were determined after 7 and 14 *** sterile wastes of pineapple,sweet potato and watermelon recorded initial percentage protein contents of 4.37,4.39,and 10.89,respectively,whereas their corresponding non-sterile wastes recorded initial percentage protein contents of 3.76,4.00,and 10.16,*** results further show that percentage increase in protein content of sterile wastes after 14 days of fermentation were pineapple,35.01%;sweet potato,27.60%;and watermelon,64.40%.Percentage increase in protein content of non-sterile wastes after 14 days of fermentation were pineapple,72.34%;sweet potato,85.25%;and watermelon,80.51%.These findings affirm the biotechnological potential of pineapple,sweet potato and watermelon wastes and the importance of fungi as agents for protein enrichment of agro-industrial wastes.

关键词： agro-based wastes fungal biotechnology nutrient enrichment solid-substrate fermentation value addition

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