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Characterization and Functionalisation of activated carbon for the Enhancement of Enzyme Catalyst Activity

材料科学与工程（中英文B版） 2021年第2期11卷 81-87页

作者： Nassereldeen A Kabbashi Yara Hunud Abia Kadouf Ibrahim Adebayo Bello Elwathig M.Saeed Lubna Mohamed Musa Nassereldeen Ahmed Kabbashi Bioenvironmental Engineering Research Centre(BERC) Department of Biotechnology Engineering(BTE)Kulliyyah of Engineering(KOE)International Islamic University Malaysia(IIUM)P.O.Box 10Gombak 50728KLMalaysia International Institute for Halal Research and Training(INHART) IIUM GombakKLMalaysia Department of Horticultural Sciences Faculty of AgricultureGezira UniversitySudan 不详

Background and objective:activated carbon is commonly used as an immobilisation matrix due to its large surface area,making it a highly desirable matrix for use in immobilising enzymes as preparation for use on the industrial *** objective of this research is to determine the effectiveness of different acids for functionalisation on immobilisation capacity and also to characterize the functionalized activated carbon for the functional groups *** and methods:activated carbon was functionalised with three acids(hydrochloric acid,nitric acid and sulphuric acid)along with a control sample washed with distilled *** capacity was calculated with hydrochloric acid functionalized activated carbon(HCl-FAC)giving the highest immobilization capacity(6.022 U/g).Characterisation of the functionalised activated carbon was conducted using FT-IR(Fourier Transform Infra-Red)spectroscopy analysis of the samples with the aim of analyzing the various functional groups present to determine the sample with distinct characteristics thus telling the degree of adsorption of lipase onto the activated carbon ***:HNO3-FAC(functionalized activated carbon)showed a very distinct pattern as a larger number of surface functional groups *** immobilisation on a matrix ensures thermal stability and increased reusability of the ***,in this research,lipase sourced from Candida antarctica was immobilised on acid functionalised activated *** best acid for functionalisation was found to be hydrochloric ***:Due to the very distinct patterns shown by the FT-IR spectrum of the HNO3-FAC after a fair comparison with others,it allows for a larger number of surface functional groups which will definitely enhance the stability of the enzyme lipase.

关键词： activated carbon characterization functionalized activated carbon enzyme activity

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