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Natural variation in the NAC transcription factor NONripening contributes to melon fruit ripening

Journal of Integrative Plant Biology 2022年第7期64卷 1448-1461页

作者： Jinfang Wang Shouwei Tian Yongtao Yu Yi Ren Shaogui Guo Jie Zhang Maoying Li Haiying Zhang Guoyi Gong Min Wang Yong Xu National Watermelon and Melon Improvement Center Beijing Academy of Agricultural and Forestry SciencesKey Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China)Beijing Key Laboratory of Vegetable Germplasm ImprovementBeijing 100097China Sanya Institute Hainan Academy of Agricultural SciencesHaikou 572025China

ThThe NAC transcription factor NONripening(NOR)is a master regulator of climacteric fruit ***(Cucumis melo L.)has climacteric and nonclimacteric fruit ripening varieties and is an ideal model to study fruit *** natural CmNAC-NOR variants,the climacteric haplotype CmNAC-NOR^(S,N) and the non-climacteric haplotype CmNAC-NOR^(A,S),have effects on fruit ripening;however,their regulatory mechanisms have not been ***,we report that a natural mutation in the transcriptional activation domain of CmNAC-NORS,Ncontributes to climacteric melon fruit ***-NOR knockout in the climacteric-type melon cultivar“BYJH”completely inhibited fruit ripening,while ripening was delayed by 5-8 d in heterozygous cmnac-nor mutant *** AC-NOR directly activated carotenoid,ethylene,and abscisic acid biosynthetic genes to promote fruit coloration and ***,CmNAC-NOR mediated the transcription of the“CmNAC-NOR-CmNAC73-CmCWINV2”module to enhance flesh *** transcriptional activation activity of the climacteric haplotype CmNAC-NORS,Non these target genes was significantly higher than that of the nonclimacteric haplotype CmNAC-NOR^(A,S).Moreover,CmNAC-NORS,Ncomplementation fully rescued the non-ripening phenotype of the tomato(Solanum lycopersicum)cr-nor mutant,while CmNAC-NOR^(A,S) did *** results provide insight into the molecular mechanism of climacteric and non-climacteric fruit ripening in melon.

关键词： climacteric fruit fruit ripening melon NAC tran-scription factor

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MaBEL1 regulates banana fruit ripening by activating cell wall and starch degradation-related genes

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Journal of Integrative Plant Biology 2023年第9期65卷 2036-2055页

作者： Zunyang Song Xiaoyang Zhu Xiuhua Lai Hangcong Chen Lihua Wang Yulin Yao Weixin Chen Xueping Li Guangdong Provincial Key Laboratory of Postharvest Science of fruits and Vegetables/Engineering Research Center for Postharvest Technology of Horticultural Crops in South China Ministry of EducationCollege of HorticultureSouth China Agricultural UniversityGuangzhou 510642China Key Laboratory of Food Processing Technology and Quality Control in Shandong Province College of Food Science and EngineeringShandong Agricultural UniversityTai'an 271018China

Banana is a typical subtropical fruit, sensitive to chilling injuries and prone to softening ***, the underlying regulatory mechanisms of the softening disorder caused by cold stress remain obscure. Herein, we found that BEL1-LIKE HOMEODOMAIN transcription factor 1(MaBEL1)and its associated proteins regulate the fruit softening and ripening process. The transcript and protein levels of MaBEL1 were up-regulated with fruit ripening but severely repressed by the chilling stress. Moreover, the MaBEL1 protein interacted directly with the promoters of the cell wall and starch degradation-related genes, such as MaAMY3, MaXYL32, and MaEXP-A8. The transient overexpression of MaBEL1 alleviated fruit chilling injury and ripening disorder caused by cold stress and promoted fruit softening and ripening of “Fenjiao” banana by inducing ethylene production and starch and cell wall degradation. The accelerated ripening was also validated by the ectopic overexpression in ***, MaBEL1-silencing aggravated the chilling injury and ripening disorder and repressed fruit softening and ripening by inhibiting ethylene production and starch and cell wall degradation. MaABI5-like and MaEBF1, the two positive regulators of the fruit softening process,interacted with MaBEL1 to enhance the promoter activity of the starch and cell wall degradationrelated genes. Moreover, the F-box protein MaEBF1 does not modulate the degradation of MaBEL1, which regulates the transcription of MaABI5-like protein. Overall, we report a novel MaBEL1-MaEBF1-MaABI5-like complex system that mediates the fruit softening and ripening disorder in “Fenjiao” bananas caused by cold stress.

关键词： cold stress fruit ripening interaction MaBEL1 softening disorder transcriptional regulation

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Movement of ACC oxidase 3 mRNA from seeds to flesh promotes fruit ripening in apple

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Molecular Plant 2024年第8期17卷 1221-1235页

作者： Ting Wang Yi Zheng Chen Xu Yulin Deng Xinyi Hao Zicheng Chu Ji Tian Yi Wang Xinzhong Zhang Zhenhai Han Ting Wu College of Horticulture China Agricultural UniversityBeijingChina Plant Science and Technology College Bioinformatics CenterBeijing University of AgricultureBeijingChina

Xenia,the phenomenon in which the pollen genotype directly affects the phenotypic characteristics of maternal tissues(i.e.,fruit ripening),has applications in crop production and ***,the underlying molecular mechanism has yet to be ***,we investigated whether mobile mRNAs from the pollen affect the ripening and quality-related characteristics of the fruit using cross-pollination between distinct Malus domestica(apple)*** demonstrated that hundreds of mobile mRNAs originating fromthe seeds are deliveredto *** themovementof one of these mRNAs,AcC oxidase 3(MdAco3),is coordinated with fruit *** acid treatment,which can cause plasmodesmal closure,blocks MdAcO3 movement,indicating that MdAcO3 transcripts may move through the *** assess the role of mobile MdAcO3 transcripts in apple fruit,we created MdACO3-GFP-expressing apple seeds using MdACO3-GFP-overexpressing pollen for pollination and showed that MdACO3 transcripts in the transgenic seeds move to the flesh,where they promote fruit ***,we demonstrated that MdAco3 can be transported from the seeds to fruit in the fleshy-fruited species tomato and *** results underscore the potential of mobile mRNAs from seeds to influence fruit characteristics,providing an explanation for the xenia ***,our findings highlight the feasibility of leveraging diverse pollen genomic resources,without resorting to genome editing,to improve fruitquality.

关键词： fruit ripening xenia ACO3 mobile RNA seed(to flesh)

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PaPYL9 is involved in the regulation of apricot fruit ripening through ABA signaling pathway

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Horticultural Plant Journal 2022年第4期8卷 461-473页

作者： Mengxiao Jia Jing Feng Lina Zhang Shikui Zhang Wanpeng Xi College of Horticulture and Landscape Architecture Southwest UniversityChongqing 400716China Agriculture National fruit Tree Germplasm Repository Xinjiang Academy ofAgricultural SciencesLuntaiXinjiang 841600China Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of EducationChongqing 400715China

Abscisic acid(ABA)is a major regulator of non-climacteric fruit ripening;however,the role of ABA in the ripening of climacteric fruit is not ***,as a typical climacteric fruit,apricots were used to investigate the role of ABA in fruit *** on weighted gene coexpression network analysis(WGCNA)of our previous transcriptome data,we treated‘Danxing’fruit with exogenous ABA and obtained ABA receptor genes,genes related to ABA biosynthesis and signal transduction,and analyzed the response of these candidate genes to exogenous ABA during fruit ***,the full length of candidate PYLs genes were cloned,and their putative function were analyzed by phylogenetic analysis and protein structure domain *** then the function of one candidate gene PaPYL9 was verified by using transgenic ***,the response genes in transgenic tomato were screened by transcriptome sequencing,and ultimately the related regulatory network was *** results showed that the injection of exogenous 1.89 mmol·L^(-1) ABA remarkably promoted fruit coloration,and increased the color index for red grapes(CIRG)and the total soluble solids(TSS)content,but significantly decreased the firmness and titratable acid(TA)content(pripening *** NCED(9-cis-epoxycarotenoiddioxygenase)and five ABA receptor genes related to signal transduction were mined from the transcriptome data of apricot fruit through *** with the control,the expression levels of NCED1,PYL9(PYR/PYL/RCAR),SnRK2(SUCROSE NON-FERMENTING1(SNF1)-RELATED PROTEIN KINASE 2S),and ABF2(ABRE-binding bZIP transcription)were induced dramatically by ABA treatment(pfruit ***

关键词： Apricot PYLs Abscisic acid Ethylene fruit ripening

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The brassinosteroid signaling component SlBZR1 promotes tomato fruit ripening and carotenoid accumulation

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Journal of Integrative Plant Biology 2023年第7期65卷 1794-1813页

作者： Fanliang Meng Haoran Liu Songshen Hu Chengguo Jia Min Zhang Songwen Li Yuanyuan Li Jiayao Lin Yue Jian Mengyu Wang Zhiyong Shao Yuanyu Mao Lihong Liu Qiaomei Wang Key Laboratory of Horticultural Plant Growth and Development Ministry of AgricultureDepartment of HorticultureZhejiang UniversityHangzhou 310058China College of Plant Science Jilin UniversityChangchun 130062China

The plant hormone ethylene is essential for climacteric fruit ripening, although it is unclear how other phytohormones and their interactions with ethylene might affect fruit ripening. Here, we explored how brassinosteroids(BRs) regulate fruit ripening in tomato(Solanum lycopersicum) and how they interact with ethylene. Exogenous BR treatment and increased endogenous BR contents in tomato plants overexpressing the BR biosynthetic gene SlCYP90B3promoted ethylene production and fruit *** analysis indicated that the BR signaling regulators Brassinazole-resistant1(SlBZR1) and BRI1-EMS-suppressor1(SlBES1) act redundantly in fruit softening. Knocking out Sl BZR1 inhibited ripening through transcriptome reprogramming at the onset of ripening. Combined transcriptome deep sequencing and chromatin immunoprecipitation followed by sequencing identified 73 Sl BZR1-repressed targets and 203 Sl BZR1-induced targets involving major ripening-related genes, suggesting that Sl BZR1 positively regulates tomato fruit *** BZR1 directly targeted several ethylene and carotenoid biosynthetic genes to contribute to the ethylene burst and carotenoid accumulation to ensure normal ripening and quality formation. Furthermore,knock-out of Brassinosteroid-insensitive2(Sl BIN2),a negative regulator of BR signaling upstream of Sl BZR1, promoted fruit ripening and carotenoid accumulation. Taken together, our results highlight the role of Sl BZR1 as a master regulator of tomato fruit ripening with potential for tomato quality improvement and carotenoid biofortification.

关键词： brassinosteroids carotenoids ethylene fruit ripening quality improvement SlBIN2 SlBZR1 tomato

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Characteristics of fruit ripening in tomato mutant epi

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2005年第6期6卷 502-507页

作者：王中凤应铁进鲍碧丽黄晓丹 School of Biosystem Engineering and Food Science Zhejiang University Hangzhou 310029 China Department of Biological and Chemical Engineering Guangxi Institute of Technology Liuzhou 545006 China

The characteristics of fruit ripening and expression of ripening-related genes were investigated in epi, an ethylene overproduction mutant of tomato (Lycopersicon esculentum Mill.). The epi produces apparently more ethylene than its wild type VFN8 at every stage of vegetative and fruit growth and ripening; compared to VFN8, the epi fruit showed higher CO2 evolution, faster descending of chlorophyll, slightly quicker increase of carotenoid and lycopene, and faster reduction in pericarp firmness during maturation and ripening; and the mRNAs of three ripening-related genes including E8, pTOM5 and pTOM6 were at higher levels in epi. The ripening-related characteristics changing of the fruit are consistent with the increase of ethylene production and ripening-related genes expression. These results suggest that epi mutation possibly did not affect the ethylene perception and signaling during fruit ripening, and that the modified characteristics of fruit ripening possibly resulted from the ethylene over- production and increased expression of ripening-related genes.

关键词： Epinastic (epi) mutant Ethylene overproduction Ethylene signaling fruit ripening

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Over-expression of GhDWF4 gene improved tomato fruit quality and accelerated fruit ripening

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Journal of Integrative Agriculture 2015年第10期14卷 1980-1991页

作者： YE Shu-e LI Fang LI Xian-bi HONG Qi-bin ZHAI Yun-lan HU Ming-yu WEI Ting DENG Sha-sha PEI Yan LUO Ming Key Laboratory of Biotechnology and Crop Quality Improvement Ministry of Agriculture/Biotechnology Research CenterSouthwest University Citrus Research Institute Chinese Academy of Agricultural Sciences/National Citrus Engineering Research Center

Brassinosteroids（BRs）, a class of steroidal phytohormones are essential for many biological processes in plant. However, little is known about their roles in fruit development. Tomato is a highly valuable vegetable and has been adopted as the model species for studying fruit growth, development, and ripening. To understand the role of endogenous BRs in the development of tomato fruit, the expression patterns of three homologues of DWF4 gene were investigated and the transgenic tomato plants were generated in which the Gh DWF4 gene from upland cotton（Gossypium hirsutum L.） was ectopically expressed. The contents of main quality components were analyzed in fruits of transgenic tomato line and non-transgenic line（control plant, CP） when the fruit was mature. Sl CYP90B3 that possesses high homology with Gh DWF4 preferentially expressed in mature fruit. Significantly higher contents of soluble sugar, soluble proteins, and vitamin C were obtained in fruit of transgenic tomato lines compared with those in the CP. Furthermore, overexpressing Gh DWF4 promoted fruit growth and ripening. The weight per fruit was increased by about 23% in transgenic lines. In addition, overexpressing Gh DWF4 promoted the germination of transgenic tomato seeds and hypocotyl elongation of seedlings. These results indicated that overexpressing Gh DWF4 gene in tomato could increase the contents of many nutrients in fruit and accelerate fruit ripening. It is suggested that increased endogenous BRs in fruit affect the growth and development of tomato fruit and therefore improved the nutrient quality of tomato.

关键词： brassinosteroids Gh DWF4 tomato fruit ripening fruit quality transgenic plant

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ERF4 affects fruit ripening by acting as a JAZ interactor between ethylene and jasmonic acid hormone signaling pathways

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Horticultural Plant Journal 2022年第6期8卷 689-699页

作者： Yanan Hu Hualin Sun Zhenyun Han Shuai Wang Ting Wang Qiqi Li Ji Tian Yi Wang Xinzhong Zhang Xuefeng Xu Zhenhai Han Ting Wu State Key Laboratory of Agrobiotechnology College of HorticultureChina Agricultural UniversityBeijing 100193China Plant Science and Technology College Beijing University of AgricultureBeijing 102206China

The regulation of apple(Malus domestica)fruit texture during ripening is complex and a fundamental determinant of its commercial *** climacteric fruit,ripening-related processes are regulated by ethylene(ET),and jasmonate(JA)is also involved in the ethylene biosynthesis pathway,mainly through the transcription factor ***,the molecular genetic mechanism for fruit ripening processes between the JA and ET signaling pathways still needs to be *** order to explore how JA regulates apple fruit ripening through ERF4,we used’Gala’and’Ralls Janet’fruit at different developmental stages as experimental materials to determine the fruit firmness and related gene expression ***,we carried out different hormone treatments on’Gala’fruit at ripening ***,we show that ERF4 is a core JA signaling hub protein JASMONATE ZIM-DOMAIN(JAZ)interactor that affects ethylene signaling *** fruit development,ERF4 represses the expression of ACS1 and ACO1 by interacting with JAZ,as well as with the JA-activated transcription factor *** is promoted in JAZ-suppressed ***,ERF4 acts as a molecular link between ethylene and JA hormone signals,and the natural variation of the ERF4Ethylene-responsive binding factor-associated amphiphilic repression(EAR)motif decreases repression of ethylene biosynthesis genes.

关键词： Apple ERF4 Ethylene fruit ripening JAZ

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Genome-wide binding analysis of the tomato transcription factor SlDof1 reveals its regulatory impacts on fruit ripening

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Molecular Horticulture 2021年第1期1卷 90-107页

作者： Yuying Wang Peiwen Wang Weihao Wang Lingxi Kong Shiping Tian Guozheng Qin Key Laboratory of Plant Resources Institute of BotanyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100093China University of Chinese Academy of Sciences Beijing 100049China

The DNA binding with one finger(Dof)proteins are plant-specific transcription factors involved in a variety of biological ***,little is known about their functions in fruit ripening,a flowering-plant-specific process that is required for seed maturation and ***,we found that the tomato Dof transcription factor SlDof1,is necessary for normal fruit *** of SlDof1 expression by RNA interference delayed ripening-related processes,including lycopene synthesis and ethylene *** profiling indicated that SlDof1 influences the expression of hundreds of genes,and a chromatin immunoprecipitation sequencing revealed a large number of SlDof1 binding sites.A total of 312 genes were identified as direct targets of SlDof1,among which 162 were negatively regulated by SlDof1 and 150 were positively *** SlDof1 target genes were involved in a variety of metabolic pathways,and follow-up analyses verified that SlDof1 directly regulates some well-known ripening-related genes including ACS2 and PG2A as well as transcriptional repressor genes such as *** findings provide insights into the transcriptional regulatory networks underlying fruit ripening and highlight a gene potentially useful for genetic engineering to control ripening.

关键词： Tomato fruit ripening Transcriptional regulation DNA binding with one finger(Dof) chromatin immunoprecipitation followed by sequencing(ChIP-seq) RNA sequencing(RNA-seq)

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ClSnRK2.3 negatively regulates watermelon fruit ripening and sugar accumulation

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Journal of Integrative Plant Biology 2023年第10期65卷 2336-2348页

作者： Jinfang Wang Yanping Wang Yongtao Yu Jie Zhang Yi Ren Shouwei Tian Maoying Li Shengjin Liao Shaogui Guo Guoyi Gong Haiying Zhang Yong Xu State Key Laboratory of Vegetable Biobreeding National Engineering Research Center for VegetablesBeijing Key Laboratory of Vegetable Germplasms ImprovementBeijing Vegetable Research CenterBeijing Academy of Agriculture and Forestry ScienceBeijing 100097China

Watermelon(Citrullus lanatus) as non-climacteric fruit is domesticated from the ancestors with inedible fruits. We previously revealed that the abscisic acid(ABA) signaling pathway gene ClSnRK2.3 might infuence watermelon fruit ripening. However,the molecular mechanisms are unclear. Here,we found that the selective variation of ClSnRK2.3 resulted in lower promoter activity and gene expression level in cultivated watermelons than ancestors, which indicated ClSnRK2.3 might be a negative regulator in fruit ripening. Overexpression (OE) of ClSnRK2.3 significantly delayed watermelon fruit ripening and suppressed the accumulation of sucrose, ABA and gibberellin GA4. Furthermore,we determined that the pyrophosphate-dependent phosphofructokinase(ClPFP1) in sugar metabolism pathway and GA biosynthesis enzyme GA20 oxidase(Cl GA20ox) could be phosphorylated by ClSnRK2.3 and thereby resulting in accelerated protein degradation in OE lines and finally led to low levels of sucrose and GA4. Besides that, ClSnRK2.3 phosphorylated homeodomain-leucine zipper protein(ClHAT1) and protected it from degradation to suppress the expression of the ABA biosynthesis gene 9’-cis-epoxycarotenoid dioxygenase 3(Cl NCED3). These results indicated that ClSnRK2.3 negatively regulated watermelon fruit ripening by manipulating the biosynthesis of sucrose, ABA and GA4. Altogether, these findings revealed a novel regulatory mechanism in non-climacteric fruit development and ripening.

关键词： fruit ripening SnRK2.3 sucrose watermelon

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