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Identification of crucial roles of transcription factor IhfA on high production of free fatty acids in Escherichia coli

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Synthetic and Systems Biotechnology 2024年第1期9卷 144-151页

作者： Lixia Fang Ziyi Han Xueru Feng Xueyan Hao Mengxiao Liu Hao Song Yingxiu Cao Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education) School of Chemical Engineering and TechnologyTianjin UniversityTianjin300072China Frontiers Research Institute for Synthetic Biology Tianjin UniversityChina

Transcription factor engineering has unique advantages in improving the performance of microbial cell factories due to the global regulation of gene *** analyses and reverse engineering enable learning and subsequent incorporation of novel design strategies for further ***,we identify the role of the global regulator IhfA for overproduction of free fatty acids(FFAs)using CRISPRi-facilitated reverse engineering and cellular physiological *** the differentially expressed genes in the ihfAL􀀀strain,a total of 14 beneficial targets that enhance FFAs production by above 20% are identified,which involve membrane function,oxidative stress,and *** membrane-related genes,the engineered strains obtain lower cell surface hydrophobicity and increased average length of membrane lipid *** oxidative stress-related genes,the engineered strains present decreased reactive oxygen species(ROS)*** gene modulations enhance cellular robustness and save cellular resources,contributing to FFAs *** study provides novel targets and strategies for engineering microbial cell factories with improved FFAs bioproduction.

关键词： free fatty acids CRISPRi ihfA Cell membrane ROS

Effects of different free fatty acids on insulin resistance in rats

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Hepatobiliary & Pancreatic Diseases International 2008年第1期7卷 91-96页

作者： Han, Ping Zhang, Yong-Yan Lu, Yan He, Bing Zhang, Wei Xia, Fei China Med Univ Affiliated Shengjing Hosp Dept Endocrinol Shenyang 110004 Peoples R China

BACKGROUND: Much evidence demonstrates that elevated free fatty acids (FFAs) are associated with insulin resistance. However, it is not clear whether different FFAs can cause different degrees of peripheral insulin resistance. This study aimed to investigate the effects of short-term elevation of FFAs on hepatic and peripheral insulin action, and determine whether FFAs with different degrees of saturation have differential effects on hepatic insulin resistance. METHODS: Intralipid+heparin (IH, polyunsaturated fatty acids), oleate (OLE), lard oil+heparin (LOH), and saline (SAL) were separately infused intravenously for 7 hours in normal Wistar rats. During the last 2 hours of the fat/saline infusion, a hyperinsulinemic-euglycemic clamping was performed with [6-H-3] glucose tracer. Plasma glucose was measured using the glucose oxygenase method. Plasma insulin and C-peptide were determined by radioimmunoassays. Plasma FFAs were measured using a colorimetric method. RESULTS: Compared with infusion of SAL, plasma FFA levels were significantly elevated by infusions of IH, OLE, and LOH (Pfatty acids induced less hepatic insulin resistance than monounsaturated or saturated fatty acids. However, IH, OLE, and LOH infusions induced similar peripheral insulin resistance.

关键词： free fatty acids insulin resistance hepatic glucose production glucose utilization

Effect of sodium salicylate on oxidative stress and insulin resistance induced by free fatty acids

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Hepatobiliary & Pancreatic Diseases International 2010年第1期9卷 49-53页

作者： He, Bing Zhao, Sheng Zhang, Wei Li, Yan Han, Ping China Med Univ Shengjing Affiliated Hosp Dept Endocrinol Shenyang 110004 Peoples R China China Med Univ Affiliated Hosp 4 Dept Internal Med Shenyang 110032 Peoples R China

BACKGROUND: It has been reported that high-dose salicylates improve free fatty acids (FFAs)-induced insulin resistance and beta-cell dysfunction in vitro, but the mechanism remains uncertain. In insulin-resistant rats, we found that the supplementation of sodium salicylate is associated with a reduction of plasma malondialdehyde (MDA), a marker of oxidative stress. Few studies have investigated the effects of salicylates on oxidative stress levels in insulin-resistant animal models. This study aimed to assess the effect of sodium salicylate on insulin sensitivity and to explore the potential mechanism by which it improves hepatic and peripheral insulin resistance. METHODS: Intralipid+heparin (IH), saline (SAL), or intralipid+heparin+sodium salicylate (IHS) were separately infused for 7 hours in normal Wistar rats. During the last 2 hours of the infusion, hyperinsulinemic-euglycemic clamping was 3 performed with [6-(3)H] glucose tracer. Plasma glucose was measured using the glucose oxygenase method. Plasma insulin and C-peptide were determined by radioimmunoassay. MDA levels and glutathione peroxidase (GSH-PX) activity in the liver and skeletal muscle were measured with colorimetric kits. RESULTS: Compared with infusion of SAL, IH infusion increased hepatic glucose production (HGP), and decreased glucose utilization (GU) (Pfree fatty acids increased the MDA levels and decreased the GSH-PX activity in the liver and muscle (Pfatty acids induces insulin resistance by enhancing oxidative stress levels in the liver and muscle. The administration of the anti-inflammatory drug sodium salicylate reduces the degree of oxidative stress, therefore improving hepatic and peripheral insulin resistance. IKK-beta and NF-kappa B provide potential pathogenic

关键词： free fatty acids sodium salicylate oxidative stress insulin resistance hepatic glucose production

Optimization of pretreatment of Jatropha oil with high free fatty acids for biodiesel production

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Frontiers of Chemical Science and Engineering 2012年第2期6卷 210-215页

作者： Supriyono SUWITO Giuliano DRAGONE Hary SULISTYO Bardi MURACHMAN Suryo PURWONO Jose TEIXEIRA Institute for Biotechnology and Bioengineering Centre of Biological Engineering University of Minho Braga 4710-057 Portugal Chemical Engineering Department Gadjah Mada University Yogyakarta 55281 Indonesia

A central composite rotatable design and response surface methodology were used in order to investigate the individual and combined effects of the ethanol-to-oil ratio, H2SO4 concentration, temperature and time of reaction on the reduction of free fatty acid （FFA） in jatropha oil. A quadratic polynomial model relating the reaction variables with FFA reduction was developed, presenting a good coefficient of determination （R2= 0.893）. For reducing FFA to less than 1%, the optimal combination was found to be 0.62 v.v^-1 ethanol-to-oil ratio （14.9 v.v^-1 ethanol-to-FFA ratio）, 1.7% *** H2SO4 concentration, and 79 rain reaction time at a reaction temperature of 54℃. These results are of great relevance to maximize methyl esters formation by transesterification using an alkaline catalyst.

关键词： biodiesel biofuel esterification free fatty acids jatropha curcas oil

The Kinetics of the Esterification of free fatty acids in Waste Cook- ing Oil Using Fe2（SO4）3/C Catalyst

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Chinese Journal of Chemical Engineering 2009年第1期17卷 83-87页

作者：甘孟瑜潘登马利岳恩洪建兵 College of Chemistry & Chemical Engineering Chongqing University Chongqing 400044 China

The esterification of free fatty acids(FFA) in waste cooking oil with methanol in the presence of Fe2(SO4)3/C(ferric sulfate/active carbon) catalyst was *** effects of different temperature,methanol/FFA mole ratio and amount of catalyst on the conversion of FFA were *** results demonstrated that under optimal esterification conditions the final acid value of the resultant system can be reduced to ～1(mg KOH)·g-1,which met fully the requirements in post-treatment for efficient separation of glycerin and *** kinetics of the esterification were also investigated under different *** results indicated that the rate-control step could be attributed to the surface reaction and the esterification processes can be well-depicted by the as-calculated kinetic formula in the range of the experimental conditions.

关键词： biodiesel kinetics esterification free fatty acids waste cooking oil

Correlation between serum free fatty acids levels and Gensini score in elderly patients with coronary heart disease

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Journal of Geriatric Cardiology 2014年第1期11卷 57-62页

作者： Li-Yun HE Jun-Feng ZHAO Jiang-Li HAN Shan-Shan SHEN Xu-Jiao CHEN Department of Cardiology Peking University Third Hospital and Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptides Ministry ofHealth Beijing 100191 China Department of Emergency the Third Affiliated Hospital of Zhejiang Chinese Medical University Hangzhou 310005 Zhejiang Province China Department of Geriatrics Zhejiang Hospital Hangzhou 310013 Zhejiang Province China

Objectives To investigate the relationship between serum fxee fatty acids （FFAs） levels and the severity of coronary artery lesions in elderly patients with coronary heart disease （CAD）. Methods A total of 172 elderly patients who underwent coronary angiography were divided into CAD group （n = 128） and non-CAD group （n = 44） according to the results of coronary angiography. Serum FFAs and lipid levels were measured and the Gensini score were calculated. Results No matter the differences between age, gender and the usage of stat- ins or not, there was no statistical significance in FFAs levels （P 〉 0.05）. In terms of the Gensini score, it was higher in patients aged 70-79 years than in patients 60-69 years old [15.00 （5.00, 34.00） vs. 10.00 （2.00, 24.00）, P 〈 0.05], higher in men than women [14.00 （4.00, 34.00） vs. 7.00 （2.50, 19.75）, P 〈 0.05], and higher in patients on statins [13.50 （4.25, 33.50）vs. 6.50 （2.00, 18.00）, P 〈 0.05]. The serum FFAs lev- els [449.50 （299.00, 624.75） mEq/L vs. 388.00 （258.50, 495.25） mEq/L, P 〈 0.05J and Gensini score [17.50 （8.00, 41.75） vs. 1.00 （0, 5.00）, P 〈 0.05] were higher in the CAD group than in the non-CAD group. In the CAD group, there was no statistical significance in FFAs levels among patients with different numbers of diseased coronary vessels （P 〉 0.05）. Furthermore, the FFAs levels were positively correlated with the Gensini score （r = 0.394, P = 0.005）. Regression analysis showed that the FFAs levels were related to the Gensini score independently after adjusting for the other risk factors. Conclusions The serum FFAs levels were associated with the Gensini score in elderly patients with CAD. It might indicate FFAs as a biomarker predicting the severity of coronary artery lesions.

关键词： Coronary heart disease free fatty acids Gensini score The elderly

Effects of thyme(Thymus vulgaris L.)addition on the volatile compounds of mutton broth during boiling

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Food Science and Human Wellness 2022年第2期11卷 305-315页

作者： Shasha Qi Ping Zhan Honglei Tian Peng Wang Xueping Ma Kaixuan Li School of Food Science and Technology Shihezi UniversityShiheziXinjiang 832000China College of Food Engineering and Nutritional Science Shaanxi Normal UniversityXi’anShaanxi 710119China

To investigate the effects of thyme(Thymus vulgaris L.)addition on the flavor modification of mutton broth during boiling,three mutton-broth samples with various thyme contents 0.06%(S1),0.30%(S2),and1.50%(S3)were analyzed and compared,without thyme as control(0.00%,S0).The meaty,fatty,pastoral flavor and spicy were chosen as sensory attributes to evaluate the flavor of the mutton-broth *** results were significantly different(Pfatty acids(FFAs)were also identified,and all of them significantly increased with increased thyme(P<0.05).Correlation analysis of odor-active compounds,FFAs,and sensory attributes through partial least squares regression indicated the important volatiles and FFAs remarkably contributed to the mutton broth samples,and further confirmed that the 0.30%of thyme may be a desirable addition amount for the sensory characteristics of mutton broth.

关键词： Mutton broth Thyme Volatile compounds free fatty acids GC-MS PLSR

Drug-induced fatty Liver Disease:Pathogenesis and Treatment

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Journal of Clinical and Translational Hepatology 2021年第5期9卷 731-737页

作者： Tea Omanovic Kolaric Vjera Nincevic Lucija Kuna Kristina Duspara Kristina Bojanic Sonja Vukadin Nikola Raguz-Lucic George Y Wu Martina Smolic University of Osijek Faculty of MedicineOsijekCroatia University of Osijek Faculty of Dental Medicine and HealthOsijekCroatia Health Center Osijek OsijekCroatia Department of Medicine Division of Gastroenterology-HepatologyUniversity of Connecticut Health CenterFarmingtonCTUSA

Metabolic dysfunction-associated fatty liver disease(commonly known as MAFLD)impacts global health in epidemic proportions,and the resulting morbidity,mortality and economic burden is *** much attention has been given to metabolic syndrome and obesity as offending factors,a growing incidence of polypharmacy,especially in the elderly,has greatly increased the risk of drug-induced liver injury(DILI)in general,and drug-induced fatty liver disease(DIFLD)in *** review focuses on the contribution of DIFLD to DILI in terms of epidemiology,pathophysiology,the most common drugs associated with DIFLD,and treatment strategies.

关键词： Metabolic dysfunction-associated fatty liver disease Drug-induced liver injury Reactive oxygen species free fatty acids Pharmacogenetics

New insights into renal lipid dysmetabolism in diabetic kidney disease

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World Journal of Diabetes 2021年第5期12卷 524-540页

作者： Alla Mitrofanova George Burke Sandra Merscher Alessia Fornoni Peggy and Harold Katz Family Drug Discovery Center University of MiamiMiller School of MedicineMiamiFL 33136United States Katz Family Division of Nephrology and Hypertension Department of MedicineUniversity of MiamiMiller School of MedicineMiamiFL 33136United States Department of Surgery University of MiamiMiller School of MedicineMiamiFL 33136United States Diabetes Research Institute University of MiamiMiller School of MedicineMiamiFL 33136United States

Lipid dysmetabolism is one of the main features of diabetes mellitus and manifests by dyslipidemia as well as the ectopic accumulation of lipids in various tissues and organs,including the *** suggests that impaired cholesterol metabolism,increased lipid uptake or synthesis,increased fatty acid oxidation,lipid droplet accumulation and an imbalance in biologically active sphingolipids(such as ceramide,ceramide-1-phosphate and sphingosine-1-phosphate)contribute to the development of diabetic kidney disease(DKD).Currently,the literature suggests that both quality and quantity of lipids are associated with DKD and contribute to increased reactive oxygen species production,oxidative stress,inflammation,or cell ***,control of renal lipid dysmetabolism is a very important therapeutic goal,which needs to be *** article will review some of the recent advances leading to a better understanding of the mechanisms of dyslipidemia and the role of particular lipids and sphingolipids in DKD.

关键词： Diabetes Lipids free fatty acids ATP-binding cassette transporters sub-class A Sterol-O-acyltransferase 1 CD36 Sphingolipids Sphingomyelin phosphodiesterase acid-like 3b Diabetic kidney disease