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第九届全国免疫学学术大会

作者：王雨辰廉哲雄中国科学技术大学

Staphylococcus aureus is the most dangerous of all of the many common staphylococcal *** studies of immunity against Staphylococcus aureus are mostly focused on cutaneous *** infections are common,but the bacteria can spread through the bloodstream and infect distant *** we use tail vein injection mouse model to investigate the role of macrophage during blood *** ensure the critical role of macrophage during *** infection,we use clodronate liposomes to deplete macrophage before infection,and mouse became more susceptible after macrophage clearance reflected in survival rate and body *** we sacrificed the mice after infection,we found that the spleen enlargement and immune cell expansion were greatly reducing when macrophage is *** more neutrophil and myeloid inflammatory cells were ***,inflammatory cytokines in mouse serum during infection such as IL-6,MCP-1 were reduced after macrophage depletion,while higher GM-CSF at the same *** the western blot result we observed the activation of Erk and Akt signaling pathway during time after *** signaling molecule,down regulation of forkhead box protein o1 and increase of phosphorated forkhead box protein o1 were also *** investigate the role of forkhead box protein o1 in macrophage,Lysome-Cre Foxo1-Loxp mice were created to specifically deplete Foxo1 in *** these mice became more susceptible to *** *** of Foxo1 in macrophage also impaired the T cell and B cell expansion in both liver and *** cytokines like IL-1a and MCP-1 were decreased when anti-inflammatory cytokines like IL-10 is *** in vitro study showed that forkhead box protein o1 also affected the M1 and M2 polarization in bone marrow derived macrophages,retaining enormous influence on macrophage function at the same time.

关键词： Macrophage Staphylococcus aureus forkhead box protein o1

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Self-assembly of differentiated dental pulp stem cells facilitates spheroid human dental organoid formation and prevascularization

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World Journal of Stem Cells 2024年第3期16卷 287-304页

作者： Fei Liu Jing Xiao Lei-Hui Chen Yu-Yue Pan Jun-Zhang Tian Zhi-Ren Zhang Xiao-Chun Bai School of Basic Medical Sciences Southern Medical UniversityGuangzhou 510515Guangdong ProvinceChina Department of Health Management Guangdong Second Provincial General HospitalGuangzhou 510317Guangdong ProvinceChina Guangdong Provincial Key Laboratory of Tumor Interventional Diagnosis and Treatment Zhuhai People’s Hospital Affiliated with Jinan UniversityZhuhai 519000Guangdong ProvinceChina Centre of Reproduction Development and AgingFaculty of Health SciencesUniversity of MacaoMacao 999078China Department of Stomatology Guangdong Second Provincial General HospitalGuangzhou 510317Guangdong ProvinceChina Zhuhai Institute of Translational Medicine Zhuhai Hospital Affiliated with Jinan UniversityZhuhai 519000Guangdong ProvinceChina Department of Cell Biology School of Basic Medical SciencesSouthern Medical UniversityGuangzhou 510515Guangdong ProvinceChina

BACKGRoUND The self-assembly of solid organs from stem cells has the potential to greatly expand the applicability of regenerative *** cells can self-organise into microsized organ units,partially modelling tissue function and *** pulp organoids have been used to recapitulate the processes of tooth development and related ***,the lack of vasculature limits the utility of dental pulp *** To improve survival and aid in recovery after stem cell transplantation,we demonstrated the three-dimensional(3D)self-assembly of adult stem cell-human dental pulp stem cells(hDPSCs)and endothelial cells(ECs)into a novel type of spheroid-shaped dental pulp organoid in vitro under hypoxia and conditioned medium(CM).METHoDS During culture,primary hDPSCs were induced to differentiate into ECs by exposing them to a hypoxic environment and *** hypoxic pretreated hDPSCs were then mixed with ECs at specific ratios and conditioned in a 3D environment to produce prevascularized dental pulp *** biological characteristics of the organoids were analysed,and the regulatory pathways associated with angiogenesis were *** The combination of these two agents resulted in prevascularized human dental pulp organoids(Vorganoids)that more closely resembled dental pulp tissue in terms of morphology and ***-cell RNA sequencing of dental pulp tissue and RNA sequencing of Vorganoids were integrated to analyse key regulatory pathways associated with *** biomarkers forkhead box protein o1 and fibroblast growth factor 2 were identified to be involved in the regulation of *** In this innovative study,we effectively established an in vitro model of Vorganoids and used it to elucidate new mechanisms of angiogenesis during regeneration,facilitating the development of clinical treatment strategies.

关键词： Human dental pulp stem cells Prevascularized organoids Integrated analyses Angiogenesis forkhead box protein o1

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MiR-183-5p promotes the progression of non-small cell lung cancer through targeted regulation of FoXo1

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oncology and Translational Medicine 2023年第3期9卷 121-132页

作者： Yan Deng Zhengguang He Xiaobin Luo Rong Qiu Yong Zhao Wen Luo Department of Respiratory and Critical Care Medicine Suining Central HospitalSuining 629000China

objective To investigate miR-183-5p targeting to forkhead box protein o1(FoXo1)and its corresponding effect on the proliferation,migration,invasion,and epithelial-mesenchymal transition(EMT)of non-small cell lung cancer(NSCLC)*** NSCLC tissues and adjacent normal tissues from 60 patients with NSCLC adenocarcinoma were obtained via pathological biopsy or intraoperative *** cell lines were cultured in vitro,including the human normal lung epithelial cell line BEAS-2B and human NSCLC cell lines A549,SPCA-1,PC-9,and ***-183-5p and FoXo1 mRNA expression in tissues and cells were detected by qRT-PCR;the corresponding correlations in NSCLC tissues were analyzed using the Pearson test,and the relationship between miR-183-5p expression and clinicopathological parameters was *** miR-183-5p-mediated regulation of FoXo1 was verified by bioinformatics prediction alongside double luciferase,RNA-binding protein immunoprecipitation(RIP)assay,and pull-down experiments.A549 cells were divided into control,anti-miR-NC,anti-miR-183-5p,miR-NC,miR-183-5p,miR-183-5p+pcDNA3.1,and miR-183-5p+pcDNA3.1-FoXo1 *** proliferation,invasion,migration,apoptosis,and cell cycle distribution were detected using an MTT assay,clone formation assay,Transwell assay,scratch test,and flow cytometry,*** expression of EMT-related proteins in the cells was analyzed by western *** effect of miR-185-3p silencing on the development of transplanted tumors was detected by analyzing tumor formation in nude *** miR-183-5p expression was significantly higher in NSCLC tissues and cells than in adjacent normal tissues,whereas FoXo1 mRNA expression was significantly *** was a significant negative correlation between miR-183-5p and FoXo1 mRNA in NSCLC tissues(Ponally,the expression of miR-183-5p was significantly correlated with tumor size,tumor differentiation,and tumor-node-metastasis stage in patients with NSCLC(P<

关键词： non-small cell lung cancer miR-183-5p forkhead box protein o1 malignant biological behavior targeted regulation

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Naoxintong capsules(脑心通胶囊)modulates tumor necrosis factor-α-induced endothelial senescence through silent information regulator 1 signaling

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Journal of Traditional Chinese Medicine 2021年第2期41卷 212-218页

作者： GUo Qianyun ZHANG Bin ZHANG Haitong ZHANG Erli WU Yongjian State key Laboratory of Cardiovascular Disease Department of Coronary Heart Disease CenterFuwai HospitalNational Center of Cardiovascular DiseaseChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100037China

oBJECTIVE:To investigate the protective effects of Naoxintong capsules(脑心通胶囊,NXT)on tumor necrosis factor-α(TNF-α)-induced senescence inendothelial cells and its ***:Human umbilical vascular endothelial cells(HUVECs)were treated with TNF-α±NXT and assessed for silent information regulator 1(SIRT1)expression and *** were stained with beta-galactosidase to assess the levels of cellular ***1 was silenced through siRNA ***:TNF-αtreatment led to the downregulation of SIRT1,resulting in forkhead box o1(Foxo-1)acetylation,p53 acetylation and enhanced p21 *** TNF-αtreatment,higher SAβ-Gal activity ***-αenhanced the migration of HUVECs and increased SIRT1 expression,both of which were attenuated by NXT *** downstream targets of SIRT1 including Foxo-1/p53/p21 were also modulated,and HUVECs were protected from TNF-α-induced *** contrast,the NXT-mediated protection was prevented by SIRT1 ***:These findings suggest that sustained endothelial senescence can be induced by TNF-αstimulation via the SIRT1/Foxo-1/p53/p21 *** protection of NXT against TNF-αwas partially mediated through its effects on *** highlights the promise of NXT as a therapeutic for atherosclerosis.

关键词： tumor necrosis factor-alpha sirtuin 1 forkhead box protein o1 Naoxintong

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