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An interpretation of the genetic polymorphism and population genetic background of Ankang Han population via a novel InDel panel

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forensic Sciences Research 2022年第4期7卷 694-701页

作者： Wei Cui Xiaoye Jin Yating Fang Qiong Lan Jiangwei Lan Man Chen Shuyan Mei Tong Xie Bofeng Zhu Multi-Omics Innovative Research Center of forensic Identification Department of forensic Genetics School of Forensic MedicineSouthern Medical UniversityGuangzhouChina Department of forensic Medicine Guizhou Medical UniversityGuiyangChina Key laboratory of Shaanxi Province for Craniofacial Precision Medicine ResearchCollege of StomatologyXi’an Jiaotong UniversityXi’anChina

In this research,genotyping data of 43 InDel loci in 311 Han individuals in Ankang City,Shaanxi Province,China were detected using a self-developed five-dye multiplex amplification panel.The allelic frequencies and forensic parameters of all InDel loci were calculated.The combined power of discrimination and probability of exclusion values were 0.99999999999999999882739 and 0.999887424,respectively,which demonstrated that this 43-InDel panel was powerful for individual identifications in Ankang Han population.Moreover,genetic distances,pairwise F_(ST)values,principal component analyses,phylogenetic trees and STRUCTURE analyses were performed to investigate the genetic affinities between Ankang Han and reference groups.Population genetic investigations indicated that Ankang Han population had a close genetic relationship with Southern Han population compared with other reference groups.

关键词： forensic sciences forensic genetics forensic parameter InDel Population genetics Ankang Han population

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Genetic Polymorphism and Relationship Analyses of Standard Poodle and Bichon Frise Groups Based on 19 Short Tandem Repeat Loci

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Journal of forensic Science and Medicine 2023年第4期9卷 331-339,I0013-I0015页

作者： Shuyan Mei Jinlong Yang Jianping Li Xin Xiong Menglei Wang Zhichao Zhao Yuxin Guo Yajun Deng College of Basic Medicine and forensic Medicine Henan University of Science and TechnologyHenanChina Guangzhou Key Laboratory of forensic Multi‑Omics for Precision Identification School of Forensic MedicineSouthern Medical UniversityGuangzhouChina Beijing Zhongzheng DNA Evidence Institute BeijingChina Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research College of StomatologyXi’an Jiaotong UniversityXi’AnChina Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases College of StomatologyXi’an Jiaotong UniversityXi’AnChina

Context:As the increasing number of pet canines,the identification of canine has attracted much attentions in the forensic field,however,the genetic diversities of pet canines still remained unknown.Aims:To explore genetic polymorphisms of 19 short tandem repeat(STR)loci and genetic relationships between the two studied canine groups and reference group.Subjects and Methods:In the present study,genetic polymorphisms of 19 STR loci and a sex-linked zinc finger locus were analyzed in a total of 594 canines in Standard Poodle and Bichon Frise groups from China.Results:A total of 166,159 alleles were observed in the Standard Poodle,Bichon Frise groups with the corresponding allelic frequencies ranging from 0.0030-0.6108 to 0.0012-0.6148,respectively.The combined discrimination power and probability of exclusion of 19 STR loci in Standard Poodle and Bichon Frise groups were 0.9999999999999497,0.999962884;and 0.99999999999999995,0.999965955,respectively.Furthermore,the genetic distances between the two canine groups and Labrador retriever group were calculated,and the results indicated that Standard Poodle and Bichon Frise groups showed a closer genetic relationship,while the two canine groups had distant genetic relationships with Labrador retriever group.The result of population genetic structure revealed that genetic component distributions in the three canine groups were different.The predicted accuracies of the constructed random forest prediction model for three validation sets(25%individuals randomly selected from three populations with 808 individuals)were higher than 0.9,especially for the individuals in validation set from the Bichon Frise group is 1.Conclusions:The 19 STR loci could be used for individual identification,canine breed identification and paternity testing in the two canine groups.

关键词： Short tandem repeat Standard Poodle Bichon Frise forensic parameter canine genetic polymorphism

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