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Zea mays L. Pollen： An Approach to Its Quality Control

Journal of Agricultural Science and Technology(B) 2015年第8期5卷 513-522页

作者： Maria Graca Campos Norma Almaraz-Abarca] Miguel Pires Matos Nelson Mortagua Gomesl VanildaAparecida Arruda Ortrud Monika Barth Alex Silva Freitas D6bora Cristina Amancio' Ligia Bicudo Almeida-Muradian Coimbra Chemistry Center Faculty of Sciences and Technology and Faculty of Pharmacy University of Coimbra Polo 111Azinhaga de Santa Comba Coimbra 3000-548 Portugal Interdisciplinary Research Centre for Integral Regional Development-Durango National Polytechnic Institute Sigma 119 20 deNoviembre 11 Durango 34220 DGO Mexico Department of food and Experimental Nutrition Pharmaceutical Science School University of S6o Paulo Av. Prof. Lineu Prestes580 Bloco 14 CEP055800-000 Sao Paulo Brazil Instituto Oswaldo Cruz Fundaao Oswaldo Cruz Avenida Brasi14365 Rio de Janeiro 21040-900 Brazil

Zea mays L. is one of the biggest cropping systems among the sustainable development agronomy. Pollen from this crop source is unexplored and apiculture can be a good partner adding value to the product and creating new jobs helping to solve some social issues as unemployment. However, food safety is crucial, thus the aim of this study was to explore the flavonoid/phenolic profiles from Z. mays L. pollen as a fingerprint for this plant identification and also to demonstrate how the method of bee pollen samples （honeybee collected pollen） is applied. For this purpose, several sources ofZ. mays L. pollen were analyzed, including corn hybrids and genetic modified samples collected at the breeding fields. For this work, samples were taken at several years from 2000 to 2012 and collected from different countries and locations, such as Portugal, Mexico and Brazil. Results showed, for the first time, that the fingerprint （flavonoid/phenolic profile） for Z. mays L. pollen does not change over the time of sampling neither with the region of harvesting. The high performance liquid chromatography-diode array detector （HPLC/DAD） fingerprints of phenolic/flavonoid extract from Z. mays remain unchanged for all samples analyzed from different countries, hybrids and/or genetic modified plants. This is also the first study reporting these phenolic compounds not only in pollen collected directly from hybrid plants, but also in Z. mays bee pollen. The described fingerprinting method is easy, fast and accurate for the characterization of Z. mays L. pollen samples and complete microscopic analysis because it is species-specific.

关键词： Flavonoids phenols Zea mays pollen bee pollen food safely phenolic fingerprinting palynological analysis oxygenradical absorbance capacity (ORAC).

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