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The Expression of vasa Gene during Zebrafish (Danio rerio) Oogenesis

Wuhan University Journal of Natural Sciences 2004年第6期9卷 979-982页

作者： XIANGFang ZHENYan ZHENGWen-xuan DENGFeng-jiao WANGXiao-kai ZHANGXi-yuan KeyLaboratoryofMOEforDevelopmentalBiology CollegeofLifeSciencesWuhanUniversityWuhan430072HubeiChina WuhanMunicipalFourthHospital Wuhan430033HubeiChina

vasa gene expression pattern during oogenesis of zebrafish was examined usingin situ hybridization and fluorescent quantitative rt-pcr. During zebrafish oogensis,vasa mRNA is expressed strongly and uniformly distributed in the cytoplasm in stage II oocytes, followed by a distribution among vacuome in stage III. Later in stage IV and V,vasa mRNA is enriched at the cortex and finally localized at the cortex. The fluorescent quantitative rt-pcr shows that the quantity ofvasa mRNA decreases from stage II to stage III, but remains relatively invariable from stage III to stage V. The observed differences invasa mRNA expression in the different stages of zebrafish oogenesis suggest thatvasa gene plays an important role during oogenesis. Key words vasa - zebrafish - fluorescent quantitative rt-pcr - oogenesis CLC number Q 952.6 Foundation item: Supported by the National Natural Science Foundation of China (30370744, 30150005)Biography: XIANG Fang (1979-), male, Master candidate, research direction: molecular development of animals.

关键词： vasa zebrafish fluorescent quantitative rt-pcr oogenesis

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Cloning of Rabbit Bone Morphogenetic Protein 15 and Its Expression During in vitro Maturation of Rabbit Oocytes

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Zoological Research 2008年第6期29卷 603-607页

作者：尹萍季金强李霖丁家桐扬州大学动物科学与技术学院扬州225009

Partial cDNA sequence of rabbit BMP15 was cloned by rt-pcr from rabbit ovaries, showing a similarity of 83%-90% with the BMP15 nucleotide sequences in humans, mice, ovine, sheep, cows and pigs. The expression of BMP15 in rabbit cumulus-oocyte complexs during oocytes in vitro maturation （IVM） was measured by fluorescent quantitative rt-pcr method. BMP 15 was expressed at low levels in immature oocytes and increased to the highest level at 16h of IVM, which coincides with the time of cumulus cell expansion, then declined slowly under IVM cultivation. The expression pattern of BMP 15 suggested that it might be important in cumulus expansion in rabbits.

关键词： Rabbit Bone morphogenetic protein 15 Oocyte Gene cloning fluorescent quantitative rt-pcr

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