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A New Ratio-Metric pH Probe,“ThiAKS Green”for Live-Cell pH Measurements

Photonic Sensors 2023年第1期13卷 66-75页

作者： Ali AKYOL Doruk BAYKAL Akin AKDAG Özge SENSOY Çagdas Devrim SON Middle East Technical University Department of Biological SciencesAnkara 06800Turkey Middle East Technical University Department of ChemistryAnkara 06800Turkey Istanbul Medipol University School of Engineering and Natural Sciencesistanbul 34810Turkey

Deviation of the H+concentration from optimum values within the organelles is closely associated with irregular cellular functions that cause the onset of various diseases.Therefore,determining subcellular pH values in live cells and tissues is valuable for diagnostic purposes.In this study,we report a novel ratiometric fluorescence probe 1H-pyrazole-3-carboxylic acid,4-(benzo[d]thiazol-2-yl)-3-(2,4-dihydroxy-3-methylphenyl)-1H-pyrazole-5-carboxylicacid4-(2-benz othiazolyl)-5-(2,4-dihydroxy-3-methylphenyl),to which we will refer as ThiAKS Green(Thiazole AKyol shifting green),that is pH sensitive.The results presented here show that the probe can penetrate the celi membrane in iess than 30minutes and does not show any detectable toxicity.The measured color shifts up on pH change are linear and most significant around physiological pH(pKa=7.45),thus making this probe suitable for live-cell imaging and intracellular pH measurements.During the long-incubation periods following the application of the probe and the fluorescent microscopy measurements,it shows stable properties and is easy to detect in live cells.In conclusion,the results suggest that ThiAKS Green can be used to obtain precise information on the H+distribution at various compartments of the live cells.

关键词： fluorescent probe ratiometric sensor intracellular pH cell imaging fluorescent microscopy

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EFFECT OF BUNGARUS MULTICINCTUS CRUDE VENOM AND ITS COMPONENTS ON TUMOR CELLS

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Chinese Journal of Cancer Research 2003年第2期15卷 124-128页

作者：陈勇刘洁生高洁杨维东 College of Life Science & Technology Jinan University Anhui Institute of Optics and Fine Mechanics Chinese Academy of Sciences Hefei 230031

Objective: To confirm whetherBungarus multicinctus crude venom induces the apoptosis of K562 tumor cells and to find out the components inducing apoptosis of K562 cells from the crude venom. Methods: the crude venom separated and purified by cation exchange chromatography, and the effect of venoms on K562 was studied by MTT method and flow cytometry. Results: The crude venom began to kill K562 cells at than 8×l03ng/ml (the survival rate was 82.5%) concentration and the effect was more significant in 24 h when administrating 8×l05ng/ml (the survival rate was 29.4%) crude venom. Apoptotic bodies were observed in the K562 tumor cells by fluorescent microscopy after administration of 5 μg/ml cycloheximide (CHX) or the peak VI solution at about 8×105 ng/ml. The same results were detected by the flow cytometry. A sub-Gi peak appeared after administration of CHX or the sixth peak solution. Conclusion: The authors found that the venom can kill K562 tumor cells in time- and dose-dependent manner. However, the killing effect of the venom is not apoptosis. What’s more, the peak VI solution, a component of the crude venom can induce the apoptosis of K562 tumor cells.

关键词： Bungarus multicinctus crude venom Tumor cells K562 Apoptosis Toxicity MTT method fluorescent microscopy Flow cytometry

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Synthesis and Study of Two-photon Absorption Behavior of 3- (3-Arylacryloyl) -chromen-2-one Derivatives

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有机化学 2003年第Z1期23卷 409-410页

作者： SONG Hua-Can LIANG Hui-Fen SUN Yi-Feng YAO Jun-Hua School of Chemistry and Chemical Engineering Zhongshan University Guangzhou 510275 School of Chemistry and Chemical Engineering Zhongshan University Guangzhou 510275 School of Chemistry and Chemical Engineering Zhongshan University Guangzhou 510275 School of Chemistry and Chemical Engineering Zhongshan University Guangzhou 510275

　　The study on two-photon absorption materials has attracted a lot of attention in recent years, many good re search results have been obtained in the field of two-photon fluorescent microscopy and imaging. [ 1 ～ 4] It was found in our laboratory that some 3-(3-arylacryloyl)-chromen-2-one derivatives possess good two-photon absorption behav-ior, [5] therefore, a series of this family of compounds was synthesized.……

关键词： fluorescent microscopy

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