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EPR Effect of Amphiphilic Copolymer Micelles Observed by fluorescent imaging

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Chemical Research in Chinese Universities 2011年第4期27卷 628-634页

作者： LIU Tong-jun LIU Shi HU Xiu-li SHENG Shi-hou HUANG Yu-bin JING Xia-bin Department of Colorectal and Anal Surgery China-Japan Union Hospital of Jilin University Changehun 130033 P. R. China State Key Laboratory of Polymer Physics and Chemistry Changchun Institue of Applied Chemistry Chinese Academy of Sciences Changchun 130022 P.R. China

Enhanced permeation and retention（EPR） targeting effect of rhodamine B labeled PEG-b-P（LA-co-DHP） [PEG：poly（ethylene glycol）;LA：L-lactide;DHP：2,2-dihydroxylmethyl-propylene carbonate] micelles（RhB-micelles） was observed in H22 liver cancer bearing *** RhB-micelles were prepared by conjugating rhodamine B with the DHP units of amphiphilic block copolymer PEG-b-P（LA-co-DHP） followed by subsequent self-assembling of the *** parent copolymer PEG-b-P（LA-co-DHP） was synthesized by ring-opening copolymerization of LA and DHP with PEG as macroinitiator and diethyl zinc（ZnEt2） as *** micelles have a spherical shape and the average diameter is ca.50 nm by TEM（transmission electron microscope） or 80 nm by DLS（dynamic light scattering）.Their in vitro cell uptake experiment by CLSM（confocal laser scanning microscopy） and flow cytometry showed preferential internalization of micelles by MCF-7 human breast cancer cells to free *** in vivo tests by live animal imaging and ex vivo excised organ imaging showed that after vena tail injection,free RhB molecules were distributed in the whole body through the circulation system and then gradually metabolized and excreted and there was no preferential partition in tumor bed from the beginning to the *** the RhB-micelles were preferentially distributed to the tumor bed so that their concentration（fluorescent intensity） in tumor bed got the level of the liver at a certain time point between 1 and 6 h and reached a maximum relative intensity at around 12 h,indicating an obvious EPR effect of RhB-micelles in H22 liver cancer.

关键词： Enhanced permeability and retention(EPR) effect fluorescent imaging Micelle Passive targeting

Progress and Challenges of Water-soluble NIR-II Organic Fluorophores for Fluorescence imaging In vivo

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Chemical Research in Chinese Universities 2024年第2期40卷 190-201页

作者： XIE Yuxin QIN Zuojia QIAN Ming REN Tianbing YUAN Lin State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringHunan UniversityChangsha410082P.R.China

The small-molecule fluorophores for the second near-infrared(NIR-II,1000–1700 nm)window have attracted increasing attention in basic scientific research and preclinical practice owing to their deep-photo penetration,minimal physiological toxicity and simplicity of chemical ***,most of the reported small-molecule NIR-II fluorophores suffered from poor water solubility,which can easily cause organ *** addition,the aggregation caused by their poor water solubility in the aqueous solution would also result in weak fluorescence of these NIR-II ***,it is highly desirable and valuable to develop water-soluble small-molecule NIR-II fluorophores with excellent photophysical properties for high-contrast in vivo *** this review,we summarize the recent research advances in water-soluble small-molecule NIR-II fluorophores and highlight the representative bioimaging ***,the potential challenges and perspectives of water-soluble small-molecule NIR-II fluorophores are discussed as *** anticipate this review can help researchers to grab the latest information of water-soluble small-molecule fluorophores for NIR-II imaging,sequentially boosting their further development.

关键词： Organic fluorophore fluorescent imaging imaging reagent fluorescent probe The second near-infrared(NIR-II)imaging

Glucose-mediated mitochondrial reprogramming by cholesterol export at TM4SF5-enriched mitochondria-lysosome contact sites

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Cancer Communications 2024年第1期44卷 47-75页

作者： Ji Eon Kim So-Young Park Chulhwan Kwak Yoonji Lee Dae-Geun Song Jae Woo Jung Haesong Lee Eun-Ae Shin Yangie Pinanga Kyung-hee Pyo Eun Hae Lee Wonsik Kim Soyeon Kim Chang-Duck Jun Jeanho Yun Sun Choi Hyun-Woo Rhee Kwang-Hyeon Liu Jung Weon Lee Department of Pharmacy College of PharmacySeoul National UniversitySeoulRepublic of Korea Research Institute of Pharmaceutical Sciences College of PharmacySeoul National UniversitySeoulRepublic of Korea BK21 FOUR Community-Based Intelligent Novel Drug Discovery Education Unit College of Pharmacy and Research Institute of Pharmaceutical SciencesKyungpook National UniversityDaeguRepublic of Korea Department of Chemistry Seoul National UniversitySeoulRepublic of Korea College of Pharmacy Chung-Ang UniversitySeoulRepublic of Korea Natural Product Informatics Research Center Korea Institute of Science and Technology(KIST)Gangneung-siGangwon-doRepublic of Korea School of Life Sciences Gwangju Institute of Science and Technology(GIST)GwangjuRepublic of Korea Department of Biochemistry College of MedicineDong-A UniversityBusanRepublic of Korea Global AI Drug Discovery Center College of Pharmacy and Graduate School of Pharmaceutical SciencesEwha Womans UniversitySeoulRepublic of Korea Interdisciplinary Program in Genetic Engineering Seoul National UniversitySeoulRepublic of Korea

Background:Transmembrane 4 L six family member 5(TM4SF5)translocates subcellularly and functions metabolically,although it is unclear how intracellu-lar TM4SF5 translocation is linked to metabolic *** is thus of interests to understand how the traffic dynamics of TM4SF5 to subcellular endosomal membranes are correlated to regulatory roles of ***:Here,we explored the metabolic significance of TM4SF5 localization at mitochondria-lysosome contact sites(MLCSs),using in vitro cells and in vivo animal systems,via approaches by immunofluorescence,proximity labelling based proteomics analysis,organelle reconstitution ***:Upon extracellular glucose repletion following depletion,TM4SF5 became enriched at MLCSs via an interaction between mitochondrial FK506-binding protein 8(FKBP8)and lysosomal *** labeling showed molecular clustering of phospho-dynamic-related protein I(DRP1)and certain mitophagy receptors at TM4SF5-enriched MLCSs,leading to mitochondrial fis-sion and ***4SF5 bound NPC intracellular cholesterol transporter 1(NPC1)and free cholesterol,and mediated export of lysosomal cholesterol to mitochondria,leading to impaired oxidative phosphorylation but intact tri-carboxylic acid(TCA)cycle andβ-*** mouse models,hepatocyte Tm4sf5 promoted mitophagy and cholesterol transport to mitochondria,both with positive relations to liver ***:Our findings suggested that TM4SF5-enriched MLCSs regu-late glucose catabolism by facilitating cholesterol export for mitochondrial reprogramming,presumably while hepatocellular carcinogenesis,recapitulating aspects for hepatocellular carcinoma metabolism with mitochondrial repro-gramming to support biomolecule synthesis in addition to glycolytic energetics.

关键词： cholesterol fluorescent imaging glucose catabolism hepatocellular carcinogenesis mem-brane contact sites mitochondria function mitophagy oxidative phosphorylation protein-protein interaction tetraspanin

A novel off-on fluorescent probe for specific detection and imaging of cysteine in live cells and in vivo

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Chinese Chemical Letters 2020年第1期31卷 133-135页

作者： Baoxin Zhang Haijuan Zhang Miao Zhong Song Wang Qianhe Xu Dong-Hyung Cho Hongdeng Qiu State Key Laboratory of Applied Organic Chemistry and College of Chemistry and Chemical Engineering Lanzhou UniversityLanzhou 730000China CAS Key Laboratory of Chemistry of Northwestern Plant Resources/Key Laboratory for Natural Medicine of Gansu Province Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhou 730000China School of Life Sciences Kyungpook National UniversityDaegu 41566Republic of Korea

Cysteine(Cys)plays a pivotal role in many physiological and pathological processes,including detoxification and protein *** abnormal levels of Cys are linked to many *** this study,a novel red-emitting off-on fluorescent probe Cys-TCF was masterly constructed for discriminative detection of *** a series of experimental assessment,Cys-TCF displayed higher selectivity and sensitivity for Cys over other biothilols with a low detection limit(0.04μmol/L).More notably,the probe was also successfully applied to image Cys in live cells and live zebrafishes with low cytotoxicity.

关键词： Cysteine fluorescent probe Red emission Low detection limit fluorescent imaging

Au nanoparticles based ultra-fast “Turn-On” fluorescent sensor for detection of biothiols and its application in living cell imaging

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Chinese Chemical Letters 2020年第5期31卷 1083-1086页

作者： Hailiang Zhang Pengfei Xu Xintong Zhang Xiaozheng Cao Wenxiu Han Meihui Liu Xiaohui Liu Wenbin Zeng Xiangya School of Pharmaceutical Sciences Central South UniversityChangsha 410006China Institute of Clinical Pharmacy and Pharmacology Jining First People's HospitalJining Medical UniversityJining 272000China Yidu Central Hospital of Weifang Weifang 261000China

Au or other metal nanostructures have the ability to strongly quench the fluorescence of *** feature has made AuNP-conjugates attractive for the construction of platforms for various bioanalytes to overcome the limitations of small molecule fluorophores(poor solubility,long reaction time).In this paper,an ultrafast"Turn-On"fluo rescent sensor for biothiols was *** sensor is based on the fluorescent resonance energy transfer(FRET)effect between the fluorophore(PN)and AuNPs,which effectively quenches the fluorescence of the *** the presence of thiols,PN is displaced and released from AuNP surfaces,and thus,the fluorescence is rapidly *** sensor features appreciable water solubility and ultrafast response time(a few seconds for Cys).In addition,it exhibits high selectivity and a detection limit as low as 12 nmol/L for ***,the sensor presents good biocompatibility and has been successfully applied for imaging biothiols in living cells.

关键词： Gold nanoparticle FRET Biothiols Sensor fluorescent imaging

Greener Gd-doped ZnAglnS3 Quantum Dots for fluorescent and Magnetic Resonance imaging Applications

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Chemical Research in Chinese Universities 2015年第1期31卷 1-3页

作者： LIU Yang LI Dongze ZHANG Ying LIU Zhihui XIE Renguo Department of Prosthodontics Stomatological Hospital of Jilin University Changchun 130021 P. R. China Department of Respiration the First Hospital of Jilin University Changehun 130021 P. R. China State Key Laboratory of Inorganic Synthesis and Preparative Chemistry College of Chemistry Jilin University Changchun 130012 P. R. China

The combination of fluorescent imaging and magnetic resonance imaging（MRI） techniques has attracted intense attention due to the complementary imaging ability of the two modalities. Optical imaging, especially fluorescence imaging, has good sensitivity, but poor tissue penetration ability, while MRI offers anatomical details and 3D information of soft tissue with high quality in a non-invasive manner. Therefore, MRI/fluorescence dual-imaging technique should have exciting clinical potential in some application fields.

关键词： Semiconductor Quantum dot Nanocrystal Magnetic resonance imaging fluorescent imaging

A new fluorescent probe with ultralow background fluorescence for imaging of endogenous cellular selenol under oxidative stress

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Chinese Chemical Letters 2017年第10期28卷 1987-1990页

作者： Dan Cheng Yue Pan Bin-Cheng Yin Lin Yuan Xiao-Bing Zhang State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering Hunan University Changsha 410082 China Lab of Biosystem and Microanaiysis State Key Laboratory of Bioreactor Engineering East China University of Sdence and Technology Shanghai 200237 China

A new fluorescent probe（Rhod-Sec） for selenol detection with ultralow background fluorescence have been developed in this paper, which showed a 380-fold off-on fluorescence response, and the nontoxic Rhod-Sec is well suitable for detecting and imaging both exogenous and endogenous selenol in living cells. It also can be applied to visualize the fluctuation of selenol in HepG2 cells through LPS-induced cells oxidation resistance.

关键词： fluorescent probe Selenol fluorescent imaging Oxidative stress

Inflammation imaging and investigation of drug-induced hepatotoxicity with reaction based fluorescent probes for ONOO-

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Inflammation imaging and investigation of drug-induced hepat...

fluorescent traceable nanoparticles by co-self-assembly of carbon dot-drug conjugate and biodegradable hyperbranched polymer for diagnosis and therapy

第十届全国化学生物学学术会议

作者： Dan Chen Lin Yuan Xiaobing Zhang State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering Hunan University

Peroxynitrite (ONOO) is a kind of reactive oxygen species (ROS) with super activity of oxidization and nitration, and overproduction of ONOO-is associated with pathogenesis of many diseases. Thus, accurate detection of ONOO with high sensitivity and selectivity is imperative for elucidating its functions in health or disease states. Herein, we present some reaction based fluorescent probes (***-CC, Rhod-ONOO) for ONOO by combining rational design strategy and dye-screening approach[1-3]. These probes showed highly sensitive and selective response to ONOO-under physiological conditions, and were successfully used for imaging of ONOO in an inflamed mouse model and elucidating the drug-induced hepatotoxicity pathway, as well as real-time monitoring of hepatotoxicity in living animals. Therefore, these probes can be potential biological tools to explore the roles of ONOO under different physiological and pathological settings.

关键词： fluorescent probe Peroxynitrite Drug-induced liver injury fluorescent imaging

来源： cnki会议评论

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Particuology 2022年第3期20卷 79-87页

作者： Guoping Li Mingliang Pei Xinming Li Peng Liu State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical EngineeringLanzhou UniversityLanzhou 730000China

Carbon dots(CDs)have attracted more interest in tumor theranostics,but they suffer from the rapid renal clearance due to small size and high *** solve such problems,hydrophobic pH-triggered carbon dot-drug conjugate(CDs-Hy-DOX)with high doxorubicin(DOX)content of 48.23%were designed by covalent conjugation of DOX onto the CDs via acid-labile linkage with hydrazine(Hy)as *** the fluorescent traceable hybrid prodrug nanoparticles were fabricated via co-self-assembly with the CDs-Hy-DOX as pH-sensitive prodrug and a pH/reduction dual-triggered degradable hyperbranched polymer PEG-PO-Cy as polyethylene glycol(PEG)-based surfactant,as well as gatekeeper for pH/reduction dual-triggered DOX *** hybrid prodrug nanoparticles with hydrodynamic diameter of 220 nm and DOX content of 22.99%were obtained with the optimized co-self-assembling *** could release 68.98%of DOX in the simulated tumor microenvironment within 3 days in a sustained release mode,with a premature drug leakage of 7.58%.After the acid-triggered DOX release from the CDs-Hy-DOX,which was accelerated by the pH/reduction dual-triggered degradation of the hyperbranched polymer,the strong fluorescence of CDs-Hy was recovered,demonstrating the promising potential in future tumor nanotheranostics.

关键词： Tumor nanotheranostics Carbon dots Hyperbranched polymer Tumor-specific fluorescent imaging