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fluorescent Imaging of Cell Membrane Sialoglycoproteins via Metabolic labeling using Saccharide-Neuroactive Substrate

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Fluorescent Imaging of Cell Membrane Sialoglycoproteins via ...

2020年糖科学青年科技论坛

作者： Jiao Li Mengjun Tang Cheng Li Ying Zhang College of Life Sciences Northwest University

fluorescent imaging of cell membrane sialoglycoproteins in organisms,especially in the central nervous system（CNS）,is pivotal for function and mechanism ***,fewer methods could be employed for detection of sialoglycoproteins in *** developed a method for detecting sialoglycoproteins in the brain base on metabolic glycan engineering using a novel saccharide-neuroactive substrate that could pass through the blood-brain *** method was fully evaluated and has been successfully applied to the fluorescent imaging of sialoglycoproteins at live cells,which shows methodological importance for functional elucidating of brain sialoglycoproteins required for brain disease in glycomics.

关键词： Sialoglycoproteins sialic acid imaging fluorescent labeling niacin

Highly Sensitive fluorescent-labeled Probes and Glass Slide Hybridization for the Detection of Plant RNA Viruses and a Viroid

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Acta Biochimica et Biophysica Sinica 2007年第5期39卷 326-334页

作者： Zhiyou DU~(1,2), Bo JIN~3, Wenhong LIU~4, Liang CHEN~1, and Jishuang CHEN~(2,1*) 1 College of Life Sciences, Zhejiang University, Hangzhou 310029, China 2 Institute of Bioengineering, Zhejiang Sci-Tech University, Hangzhou 310018, China 3 College of Life Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China 4 College of Bioengineering, Zhejiang Chinese Medical University, Hangzhou 310053, China College of Life Sciences Zhejiang UniversityHangzhou 310029 China Institute of Bioengineering Zhejiang Sci-Tech UniversityHangzhou 310018 China College of Life Sciences Zhejiang Chinese Medical UniversityHangzhou 310053 China College of Bioengineering Zhejiang Chinese Medical UniversityHangzhou 310053 China

In this study, a modified method of the conventional RNA dot-blot hybridization was established, by replacing32p labels with CY5 labels and replacing nylon membranes with positive-charged glass slides, for detecting plant RNA viruses and a viroid. The modified RNA dot-blot hybridization method was named glass slide hybridization. The optimum efficiency of RNA binding onto the surfaces of activated glass slide was achieved using aminosilane-coated glass slide as a solid matrix and 5×saline sodium citrate （SSC） as a spotting solution. Using a CY5-labeled DNA probe prepared through PCR amplification, the optimized glass slide hybridization could detect as little as 1.71 pg of tobacco mosaic virus （TMV） RNA. The sensitivity of the modified method was four times that of dot-blot hybridization on nylon membrane with a32P-labeled probe. The absence of false positive within the genus Potyvirus [potato virus A, potato virus Y （PVY） and zucchini yellow mosaic virus] showed that this method was highly specific. Furthermore, potato spindle tuber viroid （PSTVd） was also detected specifically. A test of 40 field potato samples showed that this method was equivalent to the conventional dot-blot hybridization for detecting PVY and PSTVd. To our knowledge, this is the first report of using dot-blot hybridization on glass slides with fluorescent-labeled probes for detecting plant RNA viruses and a viroid.

关键词： glass slide hybridization fluorescent labeling dot-blot hybridization virus viroid

Synthesis of photolabile dUTP analogues and their enzymatic incorporation for DNA labeling

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Science China Chemistry 2014年第2期57卷 322-328页

作者： WU JunZhou WANG Jie TANG XinJing State Key Laboratory of Natural and Biomimetic Drugs the School of Pharmaceutical Sciences Peking University State key Laboratory of Drug Research Shanghai Institute of Materia Medica Chinese Academy of Sciences

Two new photolabile nucleotide analogues with furan-fused deoxyuridine were synthesized through Sonogashira *** enzymatic incorporation into DNA was evaluated with two DNA polymerases(Taq and Deep vent exo-)by polymerase chain reaction(PCR).Deep vent exo-recognized both nucleotides as substrates for primer extension,while Taq was much less *** irradiation of PCR products released the amino and carboxyl moieties of *** labeling with fluorescein isothiocyanate for a long DNA construct with F-dUnTP incorporation was successfully achieved.

关键词： photolabile nucleotides enzymatic incorporation nucleotide analogues fluorescent labeling

Shaping Small Bioactive Molecules to Untangle Their Biological Function： A Focus on fluorescent Plant Hormones

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Molecular Plant 2016年第8期9卷 1099-1118页

作者： Beatrice Lace Cristina Prandi Department of Chemistry University of Turin Via P. Giuria 7 10125 Torino Italy

Modern biology overlaps with chemistry in explaining the structure and function of all cellular processes at the molecular level. Plant hormone research is perfectly located at the interface between these two disciplines, taking advantage of synthetic and computational chemistry as a tool to decipher the complex biological mechanisms regulating the action of plant hormones. These small signaling molecules regulate a wide range of developmental processes, adapting plant growth to ever changing environmental conditions. The synthesis of small bioactive molecules mimicking the activity of endogenous hormones allows us to unveil many molec- ular features of their functioning, giving rise to a new field, plant chemical biology. In this framework, fluores- cence labeling of plant hormones is emerging as a successful strategy to track the fate of these challenging molecules inside living organisms. Thanks to the increasing availability of new fluorescent probes as well as advanced and innovative imaging technologies, we are now in a position to investigate many of the dynamic mechanisms through which plant hormones exert their action. Such a deep and detailed comprehension is mandatory for the development of new green technologies for practical applications. In this review, we sum- marize the results obtained so far concerning the fluorescent labeling of plant hormones, highlighting the basic steps leading to the design and synthesis of these compelling molecular tools and their applications.

关键词： plant hormones fluorescent labeling fluorescent strigolactone analogs labeled bioactive com-pounds fluorescence imaging

Mesencephalic Progenitors Can Differentiate into TH-Positive Neurons and Improve Dysfunction in PD Rats

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Tsinghua Science and Technology 2003年第4期8卷 502-507页

作者：孙朝晖赖燕来左焕琮叶智文谢佐平 Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China

This study investigated the possibility of using mesencephalic progenitors (MPs) in the treatment of Parkinson's disease (PD). MPs were prepared from E 11 13 rats and proliferated in the serum free medium with basic fibroblast growth factor (bFGF) for 10 days. Cells were then collected and implanted into the striatum only-single grafts, or simultaneously into the substantia nigra (SN) and the striatum-double grafts. Twelve weeks after transplantation, 1,1' dioctadecyl 3,3,3',3' tetramethylindocarbocyanine perchlorate (DiI), a fluorescent dye, was microinjected into the ipsilateral striatum. The results show that double grafted MPs have more potent effects on rotational behavior than single grafted MPs. Injection of the retrograde tracer DiI into the striatum results in fluorescent labeled cells within the intranigral grafts only in double graft subjects. These data suggest that MP transplants not only can improve rotational behavior, but also help to reestablish nigrostriatal connections, so that such transplants may become an efficient way of treating PD.

关键词： mesencephalic progenitor cells double grafts Parkinson's disease rotational behavior fluorescent labeling

Research on cooperative effects of the fluorescent derivatization of proteins by high-performance liquid chromatography

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Research on cooperative effects of the fluorescent derivatiz...

30th International Symposium on Capillary Chromatography、4th GC×GC Symposium、16th National Symposium and Exhibition on Chromatography,i.e. 1st Dalian International Symposium and Exhibition on Chromatography

作者： Xiaoqiang Qiao Lin Zhang Weibing Zhang Lihua Zhang~* Yukui Zhang National Chromatographic R.&.A.Center Dalian Institute of Chemical Physics The Chinese Academy of Science Dalian 116023 China

＜正＞Proteomics is a promising subject to discover potential biomarkers that could be used in the diagnosis and better understanding of human *** of challenges in proteome study is the detection of the low abundance proteins,which play important roles in the life *** to the extremely high sensitivity of fluorescence detection,fluorescence derivatization of the low abundance proteins might be an effective way to solve this problem.

关键词： fluorescent labeling Cooperative effects Protein HPLC

Facile preparation of CdSe/ligand hybrid nanospheres with good fluorescent property

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Facile preparation of CdSe/ligand hybrid nanospheres with go...

河南省化学会2012年学术年会

作者： Fangfang Dong,Jie Zhang,Yuming Guo*,Lin Yang*,Kui Wang College of Chemistry and Environmental Science,Henan Normal University,Xinxiang 453007

As one of the most important and popular semiconductors,cadmium sulfide (CdS) nanomaterials have the large direct band gap of 2.4eV at room temperature and widely used in various fields because of the excellent electr... 详细信息

关键词： CdSe cancer specific ligand hybrid nanospheres fluorescent labeling