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Micro-PIV measurements of the flow field around cells in flow chamber

Journal of Hydrodynamics 2015年第4期27卷 562-568页

作者：傅怡吴建华吴洁孙仁丁祖荣 DONG Cheng MOE Key Laboratory of Hydrodynamics and School of Naval Architecture Ocean and Civil EngineeringShanghai Jiao Tong University School of Bioscience and Bioengineering South China University of Technology Department of Biomedical Engineering The Pennsylvania State University University Park

The velocity profile around cells in a flow chamber coated with the immobilized protein and the endothelial cells is studied using the micro particle image velocimetry（PIV）. The main purpose is to study the effect of the endothelial cells on the local hydrodynamic environment and the local shear rates above a single polymorphonuclear neutrophil（PMN） and a melanoma cell when they adhere to different immobilized protein substrates. Micro-PIV images are taken in the top-view and the side-view under 10 X and 40 X objective lens and the ensemble correlation method is used to analyze the data. The results show that the endothelial monolayer has changed the patterns of the flow velocity profile of the side-view flow on the chamber bottom, and also increased the wall shear rates. The melanoma cells adhered on the immobilized fibrin disturb the local flow more than those adhered on the immobilized fibrinogen, but one sees no significant difference between the local shear rates above the PMNs adhered on the immobilized fibrinogen and those above the PMNs adhered on the immobilized fibrin.

关键词： micro-PIV flow chamber velocity profile local shear rates

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THE ANALYSIS OF THE STEADY flow IN THE flow chamber

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Journal of Hydrodynamics 1997年第2期9卷 37-45页

作者： Liu Zhao-rong, Jiang Wei-yuan, Huo yan, Qin Kai-rong, Li Xi-xi Biomechanics Laboratory, Fudan University, Shanghai 200433, P.R. China

A flow chamber whose length and width are far larger than its own height was widely used to study cell's mechanical behavior in vitro , especially the cell's adhesion property. The paper analyzed the flow field of the flow chamber in detail, and gave the analytical expressions of the velocity, pressure and shear stress when the fluid flowed in and out the chamber through a small crevice. Then, full and clear discussion of flow field was made. Also the velocity field was measured by an Ultrasonic Doppler Velocity-metre. It was found that the experimental values coincided with the theoretical values. These results were important to study the cell's mechanical behavior in vitro .

关键词： flow chamber steady flow shear stress cell's mechanical behavior

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RBC aggregation, deformation and adhesion to endothelium: Role of nitric oxide derived from L-Arginine and sodium nitroprusside

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JOURNAL OF INNOVATIVE OPTICAL HEALTH SCIENCES 2024年第5期17卷 53-65页

作者： Maksimov, M. K. Ermolinskiy, P. B. Scheglovitova, O. N. Sklyankina, N. N. Muravyov, A. V. Lugovtsov, A. E. Priezzhev, A. V. Moscow MV Lomonosov State Univ Fac Phys bld1Subbldg 2 Leninskie gory Moscow 11991 Russia NF Gamaleya Natl Res Ctr Epidemiol & Microbiol 18 Gamaleya St Moscow 123098 Russia KD Ushinsky Yaroslavl State Pedag Univ 108-1 Respublikanskaya St Yaroslavl 150000 Russia

Red blood cells (RBCs) are the most abundant human blood cells. RBC aggregation and deformation strongly determine blood viscosity which impacts hemorheology and microcirculation. In turn, RBC properties depend on different endogenous and exogenous factors. One such factor is nitric oxide (NO), which is mainly produced by endothelial cells (EC) from L-arginine amino acid in the circulatory system. Since the mechanisms of the RBC-endothelium interplay are not clear up to date and considering its possible clinical importance, the aims of this study are to investigate in vitro: (1) The effect of L-arginine induced NO on RBC aggregation and adhesion to endothelium; (2) the NO effect on RBC aggregation and deformation induced by L-arginine and sodium nitroprusside without the presence of endothelium in the samples. The RBC aggregation and adhesion to a monolayer of EC were studied using optical tweezers (OT). The RBC deformability and aggregation without endothelium in the samples were studied using the flow chamber method and Myrenne aggregometer. We confirmed that NO increases deformability and decreases aggregation of RBCs. We showed that the soluble guanylate cyclase pathway appears to be the only NO signaling pathway involved. In the samples with the endothelium, the "bell-shaped" dependence of RBC aggregation force on L-arginine concentration was observed, which improves our knowledge about the process of NO production by endothelium. Additionally, data related to L-arginine accumulation by endothelium were obtained: Necessity of the presence of extracellular L-arginine stated by other authors was put under question. In our study, NO decreased the RBC-endothelium adhesion, however, the tendency appeared to be weak and was not confirmed in another set of experiments. To our knowledge, this is the first attempt to measure the forces of RBC adhesion to endothelium monolayer with OT.

关键词： Red blood cells aggregation L-arginine endothelium optical tweezers Myrenne flow chamber RED-BLOOD-CELL VASCULAR ENDOTHELIUM SICKLE ERYTHROCYTES SHEAR-STRESS DEFORMABILITY ADHERENCE PROSTACYCLIN ELASTICITY MECHANISM STORAGE

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